Loss of heterozygosity in FANCG, FANCF and BRIP1 from head and neck squamous cell carcinoma of the oral cavity

  • Authors:
    • Christin Türke
    • Susanne Horn
    • Carola Petto
    • Dirk Labudde
    • Günter Lauer
    • Gretel Wittenburg
  • View Affiliations

  • Published online on: April 24, 2017     https://doi.org/10.3892/ijo.2017.3974
  • Pages: 2207-2220
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Abstract

Recent advances have been made in the understanding of Fanconi anemia (FA), a hereditary disease that increases the risk for head and neck squamous cell carcinomas (HNSCC) by 500- to 700-fold. FA patients harbour germline mutations in genes of cellular DNA repair pathways that are assumed to facilitate the accumulation of mutations during HNSCC development. Mutations in these FA genes may also contribute to HNSCC in general. In the present study, we analysed three FA genes; FANCF, FANCG and BRIP1, that are involved in the repair of DNA inter strand cross-links, in HNSCC and their potential role for patient survival. We measured loss of heterozygosity (LOH) mutations at eight microsatellite loci flanking three FA genes in 54 HNSCC of the oral cavity and corresponding blood samples. Survival analyses were carried out using mutational data and clinical variables. LOH was present in 17% (FANCF region), 41% (FANCG region) and 11% (BRIP1 region) of the patients. Kaplan-Meier survival curves and log-rank tests indicated strong clinical predictors (lymph node stages with decreased survival: p=2.69e-12; surgery with improved survival: p=0.0005). LOH in the FANCF region showed a weaker association with decreased overall survival (p=0.006), which however, did not hold in multivariate analyses. LOH may predominantly indicate copy number gains in FANCF and losses in FANCG and BRIP1. Integration of copy number data and gene expression proved difficult as the available sample sets did not overlap. In conclusion, LOH in FA genes appears to be a common feature of HNSCC development seen here in 57% of patients and other mutation types may increase this mutation frequency. We suggest larger patient cohorts would be needed to test the observed association of LOH in FANCF and patient survival comprehensively.

Introduction

Squamous cell carcinoma (SCC) is the most frequent tumour entity within head and neck cancers occurring in 90% of patients (1, 2) and the 6th most frequent cancer worldwide (3, 4). Despite advances in therapy, the prognosis for HNSCC patients remains poor, with a 5-year survival of 46–50% (5, 6). As exogenous factors, alcohol consumption and smoking are assumed to cause lesions and HNSCC in a dose-dependent manner (79). An infection of human papilloma virus (HPV) is present in ~25% of HNSCC and is associated with an improved prognosis (10, 11). Also, endogenous risk factors, such as genetic predisposition may promote hereditary HNSCC (12, 13).

The acquisition of somatic mutations during HNSCC development is well understood for the tumour suppressor gene TP53, which is mutated frequently in 30–78% of HNSCCs (1419). TP53 mutations are associated with a reduced radiosensitivity of HNSCC tumours and with poor prognosis (20). Other potentially important genes in HNSCC are the genes known to be mutated in Fanconi anemia (FA). Patients with this rare autosomal-recessive disorder have a 500- to 700-fold increased risk to develop HNSCC (2123) and suffer from chromosomal instability, predisposition to congenital anomalies, bone marrow failure and cancer.

FA genes are candidate genes for HNSCC

Sixteen FA genes are currently known, the gene products are involved in the surveillance and repair of DNA crosslinks (24). A recent study found germline mutations of FA genes in 27 analysed FA families (25). All FA genes are active in the S-phase of the cell cycle, where DNA damage recognized during DNA replication triggers the accumulation of FA proteins and their interaction with other repair mechanisms. In the present study, we focused on three FA genes that have not been studied extensively, the FANCF, FANCG and BRIP1.

Expression analyses using quantitative PCR showed reduced expression of all three genes, FANCF, FANCG and BRIP1 in HNSCC, with a reduction of gene expression by up to 40-fold (26). A possible cause for the reduced expression of FA genes is an allelic gene loss that can be detected as a loss of heterozygosity (LOH). Measurements of LOH have previously indicated FA genes frequently altered in oral HNSCC (2731). A frequent LOH on chromosome 9 for instance, is potentially overlapping with many genes such as tumour suppressors p16 and FANCG (32).

Clinical relevance

LOH in FA genes may impact the prognosis for HNSCC patients under different treatment regimens. It was shown that poly(ADP-ribose) polymerase (PARP) inhibitors can be used to kill specifically FANCD1 (BRCA2)-deficient tumour cells (33). Recently, the assessment of FA genes, such as FANCD2 was proposed to aid patient stratification for treatment with DNA inter-strand crosslinking agents and PARP inhibitors (34). Thus, FA genes may be used as predictive biomarkers in cancer therapy.

The three FA genes studied here

Fanconi anemia, complementation group F - FANCF is localized on chromosome 11p14.3 and involved in stabilization of multimeric FA protein complexes (35, 36). The protein, Fancf interacts with Fanca, Fancc and Fancg to stabilize the dimers of Fanca and Fancc as well as Fanca and Fancg which are core components of the FA DNA repair pathway (24, 37). Methylation of FANCF is associated with a decreased expression of the gene and with ovary, breast, lung, cervix and testis cancer (3842).

Fanconi anemia, complementation group G - FANCG (XRCC9) is localized on chromosome 9p13.3 (43). Fancg interacts with Fanca and Fancf and supports the formation of the DNA repair core complex (44). Mutations in FANCG lead to chromosomal instability in a number of different cancers (45).

The BRCA1 interacting protein C-terminal helicase 1 -BRIP1 (FANCJ) is localized on chromosome 17q22.2 (4648). BRIP1 associates with BRCA1 and thus, contributes to genomic stability through its role in cellular DNA repair (48, 49). Mutations in BRIP1 are associated with hereditary breast cancer and Fanconi anemia (50).

In this study, we established microsatellite markers for the assessment of LOH in three genetic loci comprising FA genes. To identify a potential effect on patient survival in oral HNSCC, we evaluated LOH in FA genes and clinical parameters in survival analyses. We then discuss an observed weak association of LOH in one of the three FA genes and considerable frequencies of LOH in all three genes in light of potential therapeutic relevance.

Materials and methods

Samples

All patients were enrolled for observation and treatment of HNSCC between 1993 and 2007 at the Department of Oral and Maxillofacial Surgery, University Hospital Carl Gustav Carus, Technische Universität Dresden (Dresden, Germany). Fifty-four patients, 40 male and 14 female, with a median age of 60 years were included in the study (Table I). We collected clinical information about the tumour-, node- and metastatic stage as well the radiotherapy and chemotherapy used. To study the effects of lifestyle factors we obtained data on smoking and alcohol consumption for each patient.

Table I

Clinical data for patients evaluated for loss of heterozygosity (LOH) in FA gene regions.

Table I

Clinical data for patients evaluated for loss of heterozygosity (LOH) in FA gene regions.

VariablesNo LOH (n=23)LOH FANCF region (n=9)LOH FANCG region (n=22)LOH BRIP1 region (n=6)Any LOH (n=30)All (n=54)
Age (median, 1st and 3rd quartile)59 (46;66.50)55 (53;68)59.5 (52.25;66)57 (51.25;62.75)60 (53.5;66)60 (52;66.75)
Gender
 0-Female6 (26.09%)2 (22.22%)4 (18.18%)2 (33.33%)7 (23.33%)14 (25.93%)
 1-Male17 (73.91%)7 (77.78%)18 (81.81%)4 (66, 67%)23 (76.67%)40 (74.07%)
Tumour stage
 22 (8.7%)1 (11.11%)2 (9.09%)0 (0%)2 (10%)5 (9.25%)
 32 (8.7%)2 (22.22%)6 (27.27%)1 (16.67%)8 (26.67%)10 (18.52%)
 419 (82.61%)6 (66.67%)14 (63.63%)5 (83.33%)19 (63.33%)39 (72.22%)
Node stage
 012 (52.17%)1 (11.11%)5 (22.72%)2 (33.33%)6 (20%)19 (35.19%)
 11 (4.35%)2 (22.22%)5 (22.72%)2 (33.33%)9 (30%)10 (18.52%)
 28 (34.78%)6 (66.67%)11 (50%)2 (33.33%)14 (46.67%)22 (40.74%)
 30 (0%)0 (0%)1 (4.55%)0 (0%)1 (3.33%)1 (1.85%)
Metastasis stage
 019 (82.61%)8 (88.89%)22 (100%)6 (100%)29 (96.67%)49 (90.74%)
 11 (4.35%)0 (0%)0 (0%)0 (0%)0 (0%)1 (1.85%)
Tumour grading
 11 (4.35%)0 (0%)2 (9.09%)0 (0%)2 (6.67%)3 (5.56%)
 213 (56.52%)4 (44.44%)14 (63.63%)5 (83.33%)20 (66.67%)34 (62.96%)
 33 (13.04%)3 (33.33%)4 (18.18%)0 (0%)5 (16.67%)8 (14.81%)
Recurrence
 015 (65.22%)5 (55.56%)14 (63.64%)4 (66.67%)20 (66.67%)35 (64.81%)
 18 (34.78%)4 (44.44%)8 (36.36%)2 (33.33%)10 (33.33%)19 (35.19%)
Surgery21 (91.30%)7 (77.78%)18 (81.82%)6 (100%)26 (86.67%)48 (88.89%)
Radiotherapy21 (91.30%)9 (100%)20 (90.90%)6 (100%)28 (93.33%)49 (90.74%)
Chemotherapy8 (34.78%)4 (44.44%)5 (22.73%)1 (16.67%)7 (23.33%)15 (27.78%)
Nicotin/Alcohol18 (78.26%)7 (77.78%)19 (86.36%)3 (50%)25 (83.33%)43 (79.63%)
Survival (months, median, 1st and 3rd Qu.)21.33 (13.61; 73.77)10.30 (7.53; 22.13)20.40 (8.30; 98.21)40.73 (16.10; 73.72)19.48 (7.80; 78.31)20.60 (11.30; 78.80)

Tumour specimens were initially chosen to represent primary HNSCC tumours of the locally advanced stages T3 and T4 (51) and were collected in surgeries and biopsies. Five tumour samples were later re-assigned to stage T2. Tumour and corresponding blood samples were collected during the time of surgery or biopsy, snap-frozen in liquid nitrogen and stored at −80°C. Written informed consent was obtained from all the patients.

Ethics statement

The study was approved by the ethics committee of the Faculty for Medicine at the Technische Universität Dresden, Germany (study ID EK37022001).

DNA extraction, microsatellite amplification and detection of LOH

DNA extraction from tumour samples and corresponding blood was performed with the QIAamp DNA Mini kit (Qiagen, Hilden, Germany) following standard procedures. Eight microsatellite markers were used to determine heterozygosity and microsatellite instability (MSI) using their polymorphic repeat length. We used eight pairs of oligonucleotide primers (Applied Biosystems, Darmstadt, Germany) which were previously published (Table II) in polymerase chain reaction (PCR) to amplify loci in proximity to three FA genes (Fig. 1) (43, 44, 5259). Six loci were repeats of dinucleotides (D9S1853, D9S171, D9S163, D11S1359, D17S1855 and D17S1607) and two were repeats of tetranucleotides (D11S1392 and D11S1981). We established three multiplex PCR reactions and one singleplex PCR. The reactions contained primers, MgCl2 (concentrations given in Table III), and 100 ng of template DNA in the Qiagen Multiplex PCR kit (Qiagen) and were run in a total reaction volume of 25 µl on a PCR cycler (Bio-Rad Laboratories, München, Germany). After an initial heating step at 95°C for 15 min, PCR cycling was carried out at 95°C for 35 sec, 56°C for 90 sec and 72°C for 90 sec for 27 cycles. Fluorescent labelling of one primer each per pair was utilized to detect the amplification products in capillary gel electrophoresis using a 3130xl Genetic Analyzer, the GeneScan 500 LIZ size standard and software GeneMapper version 4.0 (all Applied Biosystems).

Table II

Primers used for amplification of microsatellite markers.

Table II

Primers used for amplification of microsatellite markers.

PrimerSequenceLabelProduct size (bp)Refs.
D9S171 s AGCTAAGTGAACCTCATCTCTGTCTVTC158–177(5254)
D9S171 as ACCCTAGCACTGATGGTATAGTCT
D9S1853 s GATCCAGCCTCACTGAA6-FAM247–265(44, 52)
D9S1853 as TTGGGCATAGAATTTTTACTTT
D9S163 s TGCTGCACATCTTAGGGAGTNED270–271(52, 55)
D9S163 as ACAGCGCTCAGAAATCATATAA
D11S1359 s TTGGAAGACACATGCACAAANED148(43)
D11S1359 as ATTTTCCAGCCTCCATAATC
D11S1981 s AATTCCTTTACTCCAGAAAGGVTC134–178(56)
D11S1981 as CAGATTTCTGCTTTCCCAGA
D11S1392 s TTGCATCCATACGGAAAGTC6-FAM200–220(57)
D11S1392 as ACATCTGAGACTTGTAGTAGAAGGC
D17S1607 s CAGATAAAAAACACAAGTTTCTGACNED103–123(58)
D17S1607 as GCTCCACCCCAGACCTA
D17S1855 s GGGGACCNCTAGAAACCPET219–225(52, 58)
D17S1855 as GAGAATACATTGTAACAACTCCAGT

[i] Label, fluorescent labeling of the forward primers; S, sense; As, antisense.

Table III

Combinations of primers and concentrations for multiplex PCR.

Table III

Combinations of primers and concentrations for multiplex PCR.

CombinationPrimer namePrimer concentrationMgCl2 concentration
1D9S1710.20 µM1.8 mM
D9S1630.15 µM
D17S16070.30 µM
2D11S13920.20 µM1.5 mM
D11S13590.20 µM
3D17S18550.20 µM1.5 mM
D11S19810.20 µM
4D9S18530.20 µM1.5 mM

Larger microsatellite markers may show LOH more frequently than shorter markers, because they amplify less well in PCR. To avoid this problem we used a high amount of input DNA (100 ng). Consequently, the larger microsatellite markers showed a lower frequency of LOH for FANCF and FANCG and a higher frequency of LOH for BRIP1 when compared to the smaller markers in the same locus (Table IV). When comparing the two alleles of each microsatellite marker, the LOH affected the larger allele in 33% of the markers (standard deviation 16.6). Thus, a preferential amplification of the smaller microsatellite markers is not apparent in our samples.

Table IV

Loss of heterozygosity (LOH) in FA gene regions.

Table IV

Loss of heterozygosity (LOH) in FA gene regions.

Tumour no.FANCG
FANCF
BRIP1
D9S171
158–177 bp
D9S1853
247–265 bp
D9S163
270–271 bp
D11S1981
134–178 bp
D11S1359
148 bp
D11S1392
200–220 bp
D17S1607
103–123 bp
D17S1855
219–225 bp
2LOHLOHn.i.i.i.i.i.i.
3n.i.i.i.i.i.i.i.i.
7LOHn.i.LOHi.i.n.i.i.i.
14i.i.i.LOHLOHi.LOHn.i.
20i.n.i.i.i.i.i.i.i.
23i.LOHi.i.i.i.n.i.i.
26LOHn.i.n.i.LOHLOHLOHi.i.
27i.i.i.n.i.i.n.i.n.i.i.
30i.n.i.i.i.i.i.i.i.
37i.i.n.i.n.i.n.i.n.i.i.i.
40i.i.i.i.n.i.i.n.i.i.
48n.i.LOHi.i.i.i.i.i.
53LOHLOHLOHi.i.i.n.i.i.
60i.i.n.i.i.i.i.n.i.LOH
101i.i.i.i.i.i.i.i.
107i.i.i.i.LOHLOHi.n.i.
114i.n.i.n.i.i.i.i.i.n.i.
116LOHn.i.LOHn.i.LOHn.i.LOHi.
117i.i.n.i.i.n.i.i.n.i.i.
118LOHn.i.n.i.i.n.i.n.i.n.i.i.
120i.i.n.i.i.i.i.i.i.
121n.i.i.i.n.i.n.ii.i.i.
123LOHLOHi.n.i.i.i.i.i.
144LOHLOHi.i.i.i.i.i.
145i.n.i.LOHi.n.i.i.i.n.i.
150LOHLOHLOHi.i.MSIi.n.i.
152i.i.i.i.i.i.i.n.i.
154LOHLOHLOHi.i.i.i.i.
155n.i.i.n.i.i.i.n.i.n.i.LOH
157n.i.i.i.i.i.n.i.i.i.
171i.n.i.i.LOHLOHLOHi.i.
179i.n.i.i.i.n.i.i.i.i.
180n.i.i.n.i.i.i.i.n.i.LOH
181i.n.i.n.i.i.i.n.i.i.i.
185i.i.n.i.i.i.i.i.i.
193n.i.i.in.i.n.i.LOHi.i.
196i.i.n.i.i.i.i.i.i.
206i.i.i.n.i.i.i.n.i.i.
213i.i.n.i.i.i.i.i.n.i.
325LOHLOHLOHi.n.i.i.i.i.
326LOHn.i.n.i.i.i.i.i.i.
328LOHn.i.LOHi.n.i.n.i.i.i.
336i.i.i.i.n.i.i.i.i.
385LOHi.LOHi.i.i.i.i.
386n.i.LOHLOHi.n.i.i.i.i.
393n.i.n.i.i.i.i.LOHi.i.
401n.i.i.i.i.i.i.i.i.
409i.i.i.i.i.i.i.i.
457i.n.i.i.i.i.i.i.i.
458n.i.LOHn.i.n.i.n.i.LOHn.i.i.
474LOHLOHn.i.i.i.i.i.i.
477n.i.n.i.i.i.i.i.n.i.i.
478LOHn.i.LOHi.i.i.LOHn.i.
479LOHLOHLOHLOHLOHn.i.i.MSI
∑ i.42/5437/5437/5446/54%41/5444/5442/5446/54
77,78%68,52%68,52%85,1975,93%81,48%77,78%85,19%
∑ LOH17/4213/3712/374/466/416/443/423/46
40,48%35,14%32,43%8,69%14,63%13,64%7,14%6,52%

[i] Length of amplicons is given for each microsatellite marker. N.i., not informative; I, informative; MSI, microsatellite instability.

To detect LOH all informative microsatellite markers were analysed for their peak area (6062). The peak area of the higher peak was divided by the area of the lower peak, and a quotient of the values for tumour and blood was calculated. When above 1.5, we called LOH in the tumour tissue as previously described (62). Non-informative and unstable (MSI) markers were excluded.

Statistical analysis of clinical data

Clinical data were obtained from the regional clinic cancer registry Dresden (Table I). To investigate if LOH was more frequent in subgroups of the patients we tested for an association of LOH with age (above vs. below 60 years), gender, tumour stages (T2, T3 and T4), smoking, alcohol consumption and recurrent disease (Table I). For this we used Chi-square tests and Fisher's exact tests if expected frequencies were <5 using IBM SPSS Statistics version 19 (IBM, Ehningen, Germany).

We further investigated the potential impact of LOH on patient survival R v. 3.0. (63). Survival time was obtained and right censored for alive subjects and also if death occurred not due to HNSCC. First, we tested for an association between LOH in regions around the FA genes, the mentioned clinical parameters and overall patient survival using Kaplan-Meier curves and log-rank tests (Table V). We reported raw P-values using a significance level alpha of 0.05 as well as corrected significance levels (alpha') depending on the number of tests carried out according to the Bonferroni method (64).

Table V

P-values from log-rank tests comparing survival in HNSCC patients related to clinical variables and LOH at FA gene regions.

Table V

P-values from log-rank tests comparing survival in HNSCC patients related to clinical variables and LOH at FA gene regions.

Test no.VariablesAll patients
Patients with tumour stage T4
P-valuenP-valuen
1Gender0.495530.81939
2Tumour stage0.63653
3Node stage 2.69e-1251 2.58e-0937
4Metastasis stage0.662490.86836
5Tumour grading0.0418440.10832
6Surgery 0.000466530.0036839
7 Radiotherapy0.246530.19739
8 Chemotherapy0.0742530.13339
9 Nicotin/Alcohol0.24530.43439
10D11S19810.19450.28634
11D11S13590.0248400.061732
12D11S13920.0326420.0025431
13FANCF region0.00617520.0059538
14D9S1710.344410.90231
15D9S18530.901360.30927
16D9S1630.438350.57326
17FANCG region0.682530.45239
18D17S16070.983410.87631
19D17S18550.768440.58630
20BRIP1 region0.762530.53739
21>1 FA gene region0.6530.48339

[i] Bold indicates significance for raw P-values. Italics indicates P-values significant after correcting for multiple tests (alpha'=0.0024 for 21 tests and alpha'=0.0038 for 13 tests omitting the individual microsatellite markers).

To investigate the combined effect of several variables on patient survival we employed Cox proportional hazards (PH) regression models in R v. 3.0. (63). We estimated the proportional hazards for different sets of variables on survival, firstly using all variables in one Cox PH model. Secondly, we tested all variables which were significant in the first model. A third test used backwards elimination, starting with all and deleting the least significant variable at each step until reaching a stage where all remaining variables were significant. Since smoking and alcohol consumption coincided in many patients and both are seen as mutagenic substances we merged them into one binary variable (absence/presence) for survival analyses. For analyses of LOH and survival in Cox PH we evaluated each FA gene region independently. However, we did not evaluate each microsatellite marker independently since those showed similar results as the corresponding FA gene regions in log-rank tests.

Analysis of copy number data

From a published dataset of 106 HNSCC genotyped on microarrays (18) we extracted called copy number variants (CNV) that spanned the genes FANCF, FANCG and BRIP1.

If the reported ploidy deviated from 2 and was <1.8 or >2.2, we noted a loss or a gain, respectively.

Expression data

We accessed publicly available expression profiles of three studies on HNSCC tumours and corresponding normal tissues in NCBI Gene Expression Omnibus. For accessions GDS2520 and GDS3838 we queried the genes of interest directly retrieving lists of expression values. Fold-change in expression was calculated by comparing the cancerous and normal tissue per each tested patient. For GSE55550 we queried for differentially expressed genes by grouping (pooled) normal tissues and tumours. Then we extracted array probes corresponding to our genes of interest from the resulting gene lists and retrieved the fold change in expression when the raw P-value was <0.05.

Differential gene expression analysis using TCGA data

We queried the Cancer Genome Atlas data for head and neck squamous cell carcinoma comprising 521 samples with RNAseq data (queried in November 2016) using the TCGA Browser v0.9 at http://tcgabrowser.ethz.ch:3839/TEST/(65). The top 200 differentially expressed genes for FANCG, FANCF and BRIP1 were subjected to the gene set enrichment analysis (66). We computed overlaps with hallmarks gene sets and with gene sets of known molecular function.

In order to identify protein partners that interact physically with FANCG, FANCF and BRIP1, a network analysis was performed using GeneMANIA (67).

Results

Detection of LOH

The information content of the microsatellite markers was high with 68–85% of informative patients per marker, in 53 of 54 patients at least one microsatellite marker was informative (Table IV). Patient 37 had to be excluded from the analysis of FANCF since none of the markers was informative here. We detected LOH in 30 of 53 (57%) patients (Table IV), 23 patients had at least one informative marker but did not show LOH.

LOH was detected most frequently in the gene region containing FANCG in 40.74% (22/54) of HNSCCs. The gene region of FANCF showed LOH in 16.98% (9/53) and the BRIP1 region was affected in 11.11% (6/54) of HNSCC. Patient 116 showed LOH in all three FA gene regions. Patients 26, 458 and 479 showed LOH in FANCG and FANCF regions. Patient 478 showed LOH in FANCG and BRIP1 and patient 14 in FANCF and BRIP1 regions.

Association analysis

We tested if the frequency of LOH was associated with age (either below or above 60 years), gender, tumour stages (T2, T3 and T4), node stage, metastasis stage, histological tumour grading, smoking or alcohol consumption and recurrent disease. None of these variables was significantly associated with an increased or decreased frequency of LOH at the FA gene regions, when combining several microsatellite markers per region (Fisher's exact tests and Chi-square tests, data not shown). Individual microsatellite markers were, moreover, not associated with any factor except for D17S1607 (in the BRIP1 region) which was associated with smoking or alcohol consumption (P= 0.029). However, this association was not significant when corrected for multiple testing (alpha' of 0.006) and LOH overall was more frequent in patients without smoking or alcohol consumption.

Survival analysis

We tested for an association of the LOH in regions of FA genes and overall patient survival using Kaplan-Meier curves and log-rank tests (Table V). Higher lymph node stages were most significantly associated with decreased survival (P=2.69e-12) and surgery was most significantly associated with improved survival (P=0.0004; Fig. 2). Node stage and surgery were significant also when corrected for multiple testing (21 variables: alpha'=0.0024 or 13 variables: alpha'=0.0038, see Table V). LOH in the FANCF region was associated with decreased survival (P=0.006; Fig. 2 and Table I) and higher tumour grading was associated with increased survival (P=0.0418; Fig. 2). However, these associations were not significant when corrected for multiple testing. LOH in FANCG and BRIP1 regions were not significantly associated with survival, accordingly LOH in more than one FA gene region was also not significantly associated with survival. Stratification for patients with tumour stage T4 showed similar associations. Only node stage remained significantly associated after correcting for multiple testing (Table V). We did not stratify for T3 and T2 patients since they were too few (≤10).

To increase our understanding of LOH in FA gene regions and survival in the context of clinical covariates we employed Cox PH regression models (Table VI). We evaluated three different Cox PH models: firstly, a model with all variables, secondly one with only those variables which were significant in the previous log-rank tests. We also used backwards elimination of variables to obtain the third Cox PH model with the most significant likelihood ratio test, Wald test and score (log-rank) test. Applying a raw significance level of alpha=0.05, surgery was always significantly associated with survival (P-values between 0.003 and 0.014) and tumour grading was significantly associated with survival when fewer variables were in the model (P-values between 0.047 and 0.019). Node stage was significantly associated only in the Cox PH model with best likelihood ratio, Wald and score tests (P=0.025). LOH in FA gene regions were not significantly associated with survival in the Cox PH models. Moreover, age, gender, tumour stage, metastatic stage, radiotherapy, chemotherapy and smoking or alcohol consumption were not significantly associated with survival. When correcting for multiple testing, applying an alpha' of 0.0167 (due to the three Cox PH models evaluated), only surgery showed a significant association with survival, most prominently in the Cox PH model with the best test statistics.

Table VI

P-values from Cox proportional hazards models.

Table VI

P-values from Cox proportional hazards models.

No.VariablesOne Cox PH model with all variables
One Cox PH model with significance in log-rank tests of Kaplan-Meier analysis
Cox PH model with best likelihood ratio, Wald and Score tests
P-valuesLCIUCIP-valuesLCIUCIP-valuesLCIUCI
1Gender0.888850.20697039.176
2Tumour stage0.574600.50989933.673
3Node stage0.343300.73288724.4150.15840.8720623.1550.02539107.13028.550
4Metastasis stage0.214200.01353526.2360.125500.0183716.319
5Tumour grading0.108080.08452012.7710.04690.096220.98380.018900.061850.7785
6Surgery0.002980.0069570.36150.01390.099750.77090.002650.047690.5267
7Radiotherapy0.970830.15517869.173
8Chemotherapy0.538660.08076037.230
9 Nicotin/Alcohol0.073820.04016511.592
10FANCF region0.105180.6318671262.3400.17230.7405153.587
11FANCG region0.375240.393851118.390
12BRIP1 region0.865620.18716473.337
13>1 FA gene region0.097010.00159117.071
14Age0.545310.91926910.455
Test statistics:
n404241
Events272827
Rsquare0.5090.3350.432
Likelihood ratio test28.42 on 14 df, P=0.0124917.15 on 4 df, P=0.00180623.19 on 4 df, P=0.0001162
Wald test24.09 on 14 df, P=0.0446917.48 on 4 df, P=0.00156122.93 on 4 df, P=0.0001308
Score (log-rank) test35.05 on 14 df, P=0.00144420.63 on 4 df, P=0.000374430.97 on 4 df, P=3.1e-06

[i] Bold indicates significance for raw P-values. Italics indicates P-values significant after correcting for multiple tests (testing three models, alpha'=0.0167). LCI, lower confidence interval (95%). UCI, upper confidence interval (95%).

We did not explore associations of survival and MSI, because of a very small sample for MSI in only two patients.

Analysis of copy number data

To analyse if LOH in FANCF, FANCG and BRIP1 may be associated with amplifications or deletions, we checked if published copy number data of HNSCC (18) showed gains or losses of these genes (Table VII). Twenty prercent of HNSCC samples showed copy number variants (CNV) spanning FANCF; 3.8% (4/105) were losses and 16.2% (17/105) were gains. FANCG was lost in 28.6% (30/105) and gained in 6.7% (7/105), in sum it was affected in 35.2% of samples. BRIP1 was lost in 31.6% (31/98) and gained in 4.1% (4/98), in sum affected in 35.7%. For comparison, TP53 showed losses in 47% (49/103) of the samples and did not show gains.

Table VII

LOH, CNV and expression of FANCF, FANCG and BRIP1 in HNSCC.

Table VII

LOH, CNV and expression of FANCF, FANCG and BRIP1 in HNSCC.

GeneLOHCNV (18)Expression
This studySumGainLoss22 HNSCC, 22 normal tissues, dataset GDS2520 (65)139 HNSCC, 16 normal tissues, dataset GSE5555017 ESCC, 17 normal tissues, dataset GDS3838 (66)
FANCF16.98%20%16.2%3.8%NAUnderexpressedUnderexpressed in14/17
FANCG40.74%35.2%6.7%28.6%Overexpressed in 17/22OverexpressedOverexpressed in 14/17
BRIP111.11%35.7%4.1%31.6%NAOverexpressedOverexpressed in 15/17

[i] LOH in HNSCC measured here and data on CNV previously published match roughly for FANCF and FANCG. BRIP1 shows LOH less often. LOH may predominantly indicate copy number gains in FANCF and losses in FANCG and BRIP1. Expression data indicate underexpression of FANCF and overexpression of FANCG and BRIP1 in HNSCC. NA, not available.

Our frequencies of LOH correspond to the frequencies of copy number variants (CNV) recently published in a genome wide screen of HNSCC for FANCF (17% LOH, 20% CNV) and FANCG (41% LOH, 35% CNV) (18). The estimates depart for BRIP1 (11% LOH, 36% CNV) possibly reflecting differences between the sample sets. However, at least for FANCF and FANCG we think that our measurement of LOH approximate the measurement of CNV in array based methods.

Analysis of expression data

One dataset, GDS2520, comprised 22 pairs of HNSCC corresponding normal tissues (68). Here, FANCG was overexpressed in 17 HNSCC samples when compared to the corresponding normal tissue. The highest change in expression was 1.6-fold. Data for FANCF and BRIP1 were not available in this dataset (Table VII).

The second dataset (GSE55550, no publication available) contained gene expression profiles of 139 HNSCC and 16 normal samples. For all of our three genes of interest, there was an array probe that showed significant differential gene expression (raw P-value<0.05). Here, FANCF was underexpressed in HNSCC compared to normal tissues and FANCG and BRIP1 were overexpressed.

A third dataset (GDS3838) compared 17 esophageal squamous cell carcinomas (ESCC) to corresponding normal tissues (69). FANCF was underexpressed in 14 of 17 samples. FANCG and BRIP1 were overexpressed in 14 of 17 and 15 of 17 samples, respectively. The few samples that showed the opposite pattern (overexpression of FANCF and underexpression of FANCG or BRIP1), did not overlap.

Differential gene expression analysis using TCGA data

In order to identify cellular signaling pathways that are affected by mutations in FANCG, FANCF and BRIP1 we performed a differential gene expression analysis of the TCGA head and neck squamous cell carcinoma samples (n=521) with subsequent gene set enrichment to identify affected pathways.

The gene set enrichment analysis for FANCG revealed a significant overlap with genes that perform transfers of ubiquitin modifications (FDR q-value 0.00005 genes such as UBE2R2 and UBE2W), a process that has been described before to play a role in the damage response of FANCG (70).

Differentially expressed genes associated with FANCG are also of ribonucleotide binding activity (FDR q-value 0.00006), such as XRCC3 which is together with FANCG involved in homologous recombination to maintain chromosome stability and repair DNA damage and also physically interacts with FANCG (see below).

The gene set enrichment analysis for FANCF revealed a highly significant overlap with genes that have transcription factor activity (FDR q-value 4.86E-022), such as a number of zinc finger proteins. However, these are somewhat inconclusive as a plethora of transcriptions factors is involved in the damage response.

The gene set enrichment analysis for BRIP1 revealed highly significant overlaps with genes involved in the G2/M checkpoint, as in progression through the cell division cycle (FDR q-value of 2.07e-41), such as e.g. BRCA2 and BARD1 (BRCA1 associated RING domain 1). Also, genes differentially expressed in association with BRIP1 are preferably genes encoding cell cycle related targets of E2F transcription factors (FDR q-value of 8.14e-30). Some of these, BRCA2 and BRCA1 also physically interact with BRIP1 (see below).

In order to complement the gene set enrichment, protein partners that interact physically with FANCG, FANCF and BRIP1 were identified in a network analysis and are shown in Fig. 3. Highly associated protein sets are members of the Fanconi anemia nuclear complex (false discovery rate FDR of 9.72e-18) and proteins involved in double-strand break repair (FDR of 1.79e-3).

Discussion

Frequent LOH in FA gene regions of HNSCC

The microsatellite markers established here were informative for almost all patients and allowed us to assess the frequency of LOH at three FA gene regions in HNSCC tumours. LOH in at least one of the analysed regions containing the genes FANCF, FANCG and BRIP1 appears to be a frequent event in HNSCC development in more than half of the cases analysed here. Such a high frequency of LOH supports an assumed role of these genes in HNSCC development, and might also influence the success of HNSCC therapy.

The frequencies of LOH in the three studied genes is much higher than that of reported point mutations (0.3–1.1%): BRIP1 was mutated in 2 of 172 skin cancers, 4 of 173 esophageal cancers and 1 of 113 upper aerodigestive tract cancers, and the mutational frequencies of FANCG and FANCF were even lower (71). This suggests minor role of point mutations, however, non-coding point mutations have not been studied and may add onto these frequencies.

Mutations in HNSCC are thought to be induced by mutagens such as tobacco smoke and alcohol (79). In the present study we observed a mild negative association between smoking or alcohol consumption and FA mutation for one microsatellite marker, suggesting that in some cases mutagenesis induced by smoking or alcohol is not responsible for HNSCC progression. The frequencies of LOH at FA gene regions reported here should be seen as a minimum estimate since the distance between FA genes and the used microsatellite markers was quite large and result in false negative assignments. The FANCG region presented with LOH in 41% of the tumours and is thus a strong candidate for further study. Similarly, the tumour suppressor CDKN2A on chromosome 9p has been implicated in cancer development, and the loss of this gene is thought to be a frequent event in various cancers and in HNSCC with an estimated 25%. A proposed progression model of HNSCC based on CGH data involved an early loss of 9p suggested ~80% of the samples (29). The LOH in this region may therefore be driven by loss of the tumour suppressor CDKN2A rather than that of FANCG. However, simultaneous loss of DNA repair mediated by Fancg may promote the accumulation of mutations and a deregulation of apoptosis at the same time (72, 73). Future studies on cancer genomes will provide data to test whether heterozygosity is lost in CDKN2A and FANCG independently, or whether the loss of FANCG is a result of LOH in CDKN2A.

The markers used previously to study LOH in the FANCF region on chromosome 11p showed similar frequencies of LOH, partially using the same microsatellite markers (18–33%) (74) and also when markers were 2–3 Mb distant from our markers (17%) (32). These studies focused on primary HNSCCs. A proposed progression model of HNSCC implies that the same gene region 11p14 is lost in up to 60% of metastatic HNSCC (29). If the loss of FANCF predominantly happens during the progression of HNSCC to the metastatic stage, the gene may already be downregulated via other mechanisms in primary HNSCC, for instance methylation (40).

Markers previously used on chromosome 17q were more than 10 Mb distant from the BRIP1 region and showed a higher LOH frequency (31%) than our estimate (11%), (32). However, another study evaluated loci around 3 Mb distant from our marker positions and did not detect LOH (74).

Thus, our data on LOH in the regions of FANCG and FANCF are in concordance with previous estimates for LOH in regions containing the studied FA genes. Reports on BRIP1 vary and it is currently difficult to conclude on the role of LOH adjacent to this gene in HNSCC.

LOH in FA genes and patient survival

Impaired DNA repair in tumour tissue due to mutated FA genes may increase the sensitivity to DNA damage by radiotherapy and even more by alkylating agents and may thus prolong patient survival (72, 73, 75, 76). However, associations of LOH at FA gene regions and patient survival were not strongly supported from our data. We observed a mild association of LOH at the FANCF region and decreased survival in our Kaplan-Meier curves and log-rank tests. The loss of FANCF as a tumour suppressor is consistent with decreased survival in the affected patients and also with decreased expression of the gene in ovary, breast, lung, cervix and testis cancer (3842). However, the weak association for FANCF is not as strong as that of known predictors for HNSCC survival, such as higher node stages and surgery (77, 78). The Bonferroni correction may be overly conservative for the Cox PH models evaluated, as these can be assumed to be positively correlated and also weaker associations might be true positive results (79). Thus, in multivariate analyses with improved statistical power, the weak associations observed here may be correct.

The observed effect of tumour grading and improved survival was very weak and may not hold true, as we had a small sample size for this trait and higher tumour grading has repeatedly been described as associated with decreased survival (8082). Our analysis may also lack statistical power since the analysed sample of 53 patients had varying clinical data. A rather uniform collection of tumours from patients with similar clinical data, e.g. all with surgery and radiotherapy, would result in a strongly improved study design. A larger sample for only chemotherapy treated individuals may be necessary to explore a potential link of chemosensitivity with BRIP1 mutation.

Copy number and expression data

From the queried datasets we found that HNSCC tumours tend to overexpress FANCG and BRIP1 (Table VII), however, often exhibit chromosomal aberrations that predominantly involve the loss of these genes. After the induction of both genes for DNA repair, the copy number loss in a progressing tumour could remove both genes and allow the accumulation of mutations. This scenario supports the assumed role of FANCG and BRIP1 as tumour suppressors. These data differ from a previous study that found reduced expression of FANCG and BRIP1 in HNSCC in tongue carcinoma (26). However, a loss of FANCG and BRIP1 would diminish expression of the genes in later stages of HNSCC or in the more aggressive tongue carcinoma. Also in other cancer types FANCG was lost more often than gained (breast: 11.5% gains, 24.1% loss; lung: 13.9% gains, 43.9% losses; and pancreas: 20.3% gains, 39.0% losses) (71). However, BRIP1 was gained more often than lost indicating differences of the various cancer types (breast: 32.8% gains, 12.3% loss; lung: 36.8% gains, 6.9% losses; and pancreas: 27.1% gains, 24.8% losses).

Intergrating copy number data and expression is somewhat inconclusive as well for FANCF as it shows underexpression in two sets of HNSCC and is affected by copy number gains in another set. Copy number gains are not characteristic for tumour suppressor genes, which FANCF was supposed to be. A decrease in gene expression, may be explained by point mutations or methylation of the gene in 15% of HNSCC as previously reported (40, 83). This could also ameliorate copy number gains that involve FANCF by chance. In other cancer types FANCF is affected by CNV in 30–40% of samples, involving gains and losses (breast: 13.4% gains, 20.4% loss; lung: 13% gains, 27.1% losses; and pancreas: 29.7% gains, 14.8% losses).

Thus, copy number mutations of FA genes are frequent in HNSCC and other cancer types. However, an improved understanding of the mechanisms leading from gene mutations to gene expression changes and a potential clinical relevance could come from data on LOH, CNV, point mutations and gene expression obtained for the same samples.

Differential gene expression analysis using TCGA data

Our pathway analyses using differential gene expression analysis with subsequent gene set enrichment and network analysis showed that physically interacting partners differ for the studied genes. FANCG and FANCF show some overlap in interacting with members of the Fanconi anemia nuclear complex while BRIP1 is rather involved in the double-strand break repair pathway.

Clinical relevance

As HNSCC is routinely treated with ionizing radiation and, less frequently, also with chemotherapy, mutations in DNA repair genes may be relevant for treatment success. Since we found LOH in FA gene regions in 57% of the patients and other mutation types may add to this frequency, a substantial proportion of HNSCC patients may be eligible for poly-adenosine diphosphate ribose polymerase (PARP) inhibition therapy. PARP inhibition impairs DNA repair selectively in cancer cells, however, not in normal somatic cells and may enter the clinics for HNSCC treatment (33, 84). In theory, cancer cells with an impaired pathway for homologous recombination (e.g. due to mutated FA genes) cannot perform double strand break repair. PARP inhibition then may be used to block the base excision repair pathway for single strand break repair as well. Unlike normal cells, cancer cells will then be sensitized to DNA damage induced by radiation and alkylating chemotherapy (85). In this regard, cells deficient in FANCA, FANCC or FANCD2 were previously found to be hypersensitive to PARP inhibition (86). Also human HNSCC cells showed enhanced cytotoxicity with radiation and PARP inhibition compared to either agent alone (87). PARP inhibitors enhanced the effect of radiotherapy in a xenograft model of human HNSCC leading to reduced tumour volume and enhanced apoptosis (88). FANCF knockdown has been shown to induce chemosensitivity in cancer cells (89, 90). Thus, further studies are needed to explore the observed association of LOH in FANCF and decreased HNSCC survival as well as the potential use of FA gene mutations as an indicator for chemo- and radiosensitivity of head and neck tumours.

In conclusion, analysing three FA gene regions, we found LOH in 57% of HNSCC tumours. LOH in FANCF showed a weak association with survival of radiotherapy and chemotherapy treated HNSCC patients. Tumours with LOH in FA genes may exhibit an altered sensitivity to cancer therapy utilizing DNA damaging agents. Thus, it is worthwhile to perform further studies screening for other types of mutations in FA genes and involving larger sample sizes to improve the statistical power of survival analysis.

Acknowledgments

We thank Diana Jünger for advice on laboratory work, Susanne Koy for discussion and Jens Plaschke for advice on statistical data analysis. We thank Janet Kelso and Sebastian Bittrich for discussion and help in preparing the manuscript.

Abbreviations:

SCC

squamous cell carcinoma

LOH

loss of heterozygosity

FA

Fanconi anemia

HPV

human papilloma virus

HNSCC

head and neck squamous cell carcinomas

PARP

polyADP-ribose polymerase

PCR

polymerase chain reaction

CNV

copy number variants

ESCC

esophageal squamous cell carcinomas

PH

proportional hazards

MSI

microsatellite instability

References

1 

Parkin DM, Bray F, Ferlay J and Pisani P: Global cancer statistics, 2002. CA Cancer J Clin. 55:74–108. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Sadick M, Schoenberg SO, Hoermann K and Sadick H: Aktuelle Standards und Fortschritte in der onkologischen Bildgebung von Kopf-Hals-Tumoren. Laryngorhinootologie. 91(Suppl 1): S27–S47. 2012. View Article : Google Scholar

3 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Kademani D: Oral cancer. Mayo Clin Proc. 82:878–887. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Bonner JA, Harari PM, Giralt J, Cohen RB, Jones CU, Sur RK, Raben D, Baselga J, Spencer SA, Zhu J, et al: Radiotherapy plus cetuximab for locoregionally advanced head and neck cancer: 5-year survival data from a phase 3 randomised trial, and relation between cetuximab-induced rash and survival. Lancet Oncol. 11:21–28. 2010. View Article : Google Scholar

6 

Forastiere A, Koch W, Trotti A and Sidransky D: Head and neck cancer. N Engl J Med. 345:1890–1900. 2001. View Article : Google Scholar

7 

Maier H, Dietz A, Gewelke U, Heller WD and Weidauer H: Tobacco and alcohol and the risk of head and neck cancer. Clin Investig. 70:320–327. 1992.PubMed/NCBI

8 

Lewin F, Norell SE, Johansson H, Gustavsson P, Wennerberg J, Biörklund A and Rutqvist LE: Smoking tobacco, oral snuff, and alcohol in the etiology of squamous cell carcinoma of the head and neck: A population-based case-referent study in Sweden. Cancer. 82:1367–1375. 1998. View Article : Google Scholar : PubMed/NCBI

9 

Viswanathan H and Wilson JA: Alcohol - the neglected risk factor in head and neck cancer. Clin Otolaryngol Allied Sci. 29:295–300. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Fakhry C, Westra WH, Li S, Cmelak A, Ridge JA, Pinto H, Forastiere A and Gillison ML: Improved survival of patients with human papillomavirus-positive head and neck squamous cell carcinoma in a prospective clinical trial. J Natl Cancer Inst. 100:261–269. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Gillison ML, Koch WM, Capone RB, Spafford M, Westra WH, Wu L, Zahurak ML, Daniel RW, Viglione M, Symer DE, et al: Evidence for a causal association between human papillomavirus and a subset of head and neck cancers. J Natl Cancer Inst. 92:709–720. 2000. View Article : Google Scholar : PubMed/NCBI

12 

Trizna Z and Schantz SP: Hereditary and environmental factors associated with risk and progression of head and neck cancer. Otolaryngol Clin North Am. 25:1089–1103. 1992.PubMed/NCBI

13 

Hecht F and Hecht BK: Cancer in ataxia-telangiectasia patients. Cancer Genet Cytogenet. 46:9–19. 1990. View Article : Google Scholar : PubMed/NCBI

14 

Levine AJ, Momand J and Finlay CA: The p53 tumour suppressor gene. Nature. 351:453–456. 1991. View Article : Google Scholar : PubMed/NCBI

15 

Blons H and Laurent-Puig P: TP53 and head and neck neoplasms. Hum Mutat. 21:252–257. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Erber R, Conradt C, Homann N, Enders C, Finckh M, Dietz A, Weidauer H and Bosch FX: TP53 DNA contact mutations are selectively associated with allelic loss and have a strong clinical impact in head and neck cancer. Oncogene. 16:1671–1679. 1998. View Article : Google Scholar : PubMed/NCBI

17 

Nylander K, Dabelsteen E and Hall PA: The p53 molecule and its prognostic role in squamous cell carcinomas of the head and neck. J Oral Pathol Med. 29:413–425. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Stransky N, Egloff AM, Tward AD, Kostic AD, Cibulskis K, Sivachenko A, Kryukov GV, Lawrence MS, Sougnez C, McKenna A, et al: The mutational landscape of head and neck squamous cell carcinoma. Science. 333:1157–1160. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Agrawal N, Frederick MJ, Pickering CR, Bettegowda C, Chang K, Li RJ, Fakhry C, Xie TX, Zhang J, Wang J, et al: Exome sequencing of head and neck squamous cell carcinoma reveals inactivating mutations in NOTCH1. Science. 333:1154–1157. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Skinner HD1, Sandulache VC, Ow TJ, Meyn RE, Yordy JS, Beadle BM, Fitzgerald AL, Giri U, Ang KK and Myers JN: TP53 disruptive mutations lead to head and neck cancer treatment failure through inhibition of radiation-induced senescence. Clin Cancer Res. 18:290–300. 2012. View Article : Google Scholar :

21 

Kutler DI, Auerbach AD, Satagopan J, Giampietro PF, Batish SD, Huvos AG, Goberdhan A, Shah JP and Singh B: High incidence of head and neck squamous cell carcinoma in patients with Fanconi anemia. Arch Otolaryngol Head Neck Surg. 129:106–112. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Kutler DI, Singh B, Satagopan J, Batish SD, Berwick M, Giampietro PF, Hanenberg H and Auerbach AD: A 20-year perspective on the International Fanconi Anemia Registry (IFAR). Blood. 101:1249–1256. 2003. View Article : Google Scholar

23 

Rosenberg PS, Socié G, Alter BP and Gluckman E: Risk of head and neck squamous cell cancer and death in patients with Fanconi anemia who did and did not receive transplants. Blood. 105:67–73. 2005. View Article : Google Scholar

24 

Walden H and Deans AJ: The Fanconi anemia DNA repair pathway: Structural and functional insights into a complex disorder. Annu Rev Biophys. 43:257–278. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Chandrasekharappa SC, Lach FP, Kimble DC, Kamat A, Teer JK, Donovan FX, Flynn E, Sen SK, Thongthip S, Sanborn E, et al: NISC Comparative Sequencing Program: Massively parallel sequencing, aCGH, and RNA-Seq technologies provide a comprehensive molecular diagnosis of Fanconi anemia. Blood. 121:e138–e148. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Wreesmann VB, Estilo C, Eisele DW, Singh B and Wang SJ: Downregulation of Fanconi anemia genes in sporadic head and neck squamous cell carcinoma. ORL J Otorhinolaryngol Relat Spec. 69:218–225. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Pfeiffer J, Maier W, Ridder GJ, Zaoui K and Birkenhäger R: LOH-profiling by SNP-mapping in a case of multifocal head and neck cancer. World J Clin Oncol. 3:24–28. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Sparano A, Quesnelle KM, Kumar MS, Wang Y, Sylvester AJ, Feldman M, Sewell DA, Weinstein GS and Brose MS: Genome-wide profiling of oral squamous cell carcinoma by array-based comparative genomic hybridization. Laryngoscope. 116:735–741. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Bockmühl U, Schlüns K, Schmidt S, Matthias S and Petersen I: Chromosomal alterations during metastasis formation of head and neck squamous cell carcinoma. Genes Chromosomes Cancer. 33:29–35. 2002. View Article : Google Scholar

30 

Koy S, Hauses M, Appelt H, Friedrich K, Schackert HK and Eckelt U: Loss of expression of ZAC/LOT1 in squamous cell carcinomas of head and neck. Head Neck. 26:338–344. 2004. View Article : Google Scholar : PubMed/NCBI

31 

Weber F, Xu Y, Zhang L, Patocs A, Shen L, Platzer P and Eng C: Microenvironmental genomic alterations and clinicopathological behavior in head and neck squamous cell carcinoma. JAMA. 297:187–195. 2007. View Article : Google Scholar : PubMed/NCBI

32 

Nawroz H, van der Riet P, Hruban RH, Koch W, Ruppert JM and Sidransky D: Allelotype of head and neck squamous cell carcinoma. Cancer Res. 54:1152–1155. 1994.PubMed/NCBI

33 

Bryant HE, Schultz N, Thomas HD, Parker KM, Flower D, Lopez E, Kyle S, Meuth M, Curtin NJ and Helleday T: Specific killing of BRCA2-deficient tumours with inhibitors of poly(ADP-ribose) polymerase. Nature. 434:913–917. 2005. View Article : Google Scholar : PubMed/NCBI

34 

Duan W, Gao L, Zhao W, Leon M, Sadee W, Webb A, Resnick K, Wu X, Ramaswamy B, Cohn DE, et al: Assessment of FANCD2 nuclear foci formation in paraffin-embedded tumors: A potential patient-enrichment strategy for treatment with DNA interstrand crosslinking agents. Transl Res. 161:156–164. 2013. View Article : Google Scholar

35 

de Winter JP, Rooimans MA, van Der Weel L, van Berkel CG, Alon N, Bosnoyan-Collins L, de Groot J, Zhi Y, Waisfisz Q, Pronk JC, et al: The Fanconi anaemia gene FANCF encodes a novel protein with homology to ROM. Nat Genet. 24:15–16. 2000. View Article : Google Scholar

36 

Ahmad SI and Kirk SH: Molecular Mechanisms of Fanconi Anemia. Landes Bioscience/Eurekah.com; 2006

37 

de Winter JP, van der Weel L, de Groot J, Stone S, Waisfisz Q, Arwert F, Scheper RJ, Kruyt FA, Hoatlin ME and Joenje H: The Fanconi anemia protein FANCF forms a nuclear complex with FANCA, FANCC and FANCG. Hum Mol Genet. 9:2665–2674. 2000. View Article : Google Scholar : PubMed/NCBI

38 

Taniguchi T, Tischkowitz M, Ameziane N, Hodgson SV, Mathew CG, Joenje H, Mok SC and D'Andrea AD: Disruption of the Fanconi anemia-BRCA pathway in cisplatin-sensitive ovarian tumors. Nat Med. 9:568–574. 2003. View Article : Google Scholar : PubMed/NCBI

39 

Olopade OI and Wei M: FANCF methylation contributes to chemoselectivity in ovarian cancer. Cancer Cell. 3:417–420. 2003. View Article : Google Scholar : PubMed/NCBI

40 

Marsit CJ, Liu M, Nelson HH, Posner M, Suzuki M and Kelsey KT: Inactivation of the Fanconi anemia/BRCA pathway in lung and oral cancers: Implications for treatment and survival. Oncogene. 23:1000–1004. 2004. View Article : Google Scholar

41 

Narayan G, Arias-Pulido H, Nandula SV, Basso K, Sugirtharaj DD, Vargas H, Mansukhani M, Villella J, Meyer L, Schneider A, et al: Promoter hypermethylation of FANCF: Disruption of Fanconi Anemia-BRCA pathway in cervical cancer. Cancer Res. 64:2994–2997. 2004. View Article : Google Scholar : PubMed/NCBI

42 

Koul S, McKiernan JM, Narayan G, Houldsworth J, Bacik J, Dobrzynski DL, Assaad AM, Mansukhani M, Reuter VE, Bosl GJ, et al: Role of promoter hypermethylation in Cisplatin treatment response of male germ cell tumors. Mol Cancer. 3:162004. View Article : Google Scholar : PubMed/NCBI

43 

de Winter JP, Waisfisz Q, Rooimans MA, van Berkel CG, Bosnoyan-Collins L, Alon N, Carreau M, Bender O, Demuth I, Schindler D, et al: The Fanconi anaemia group G gene FANCG is identical with XRCC9. Nat Genet. 20:281–283. 1998. View Article : Google Scholar : PubMed/NCBI

44 

Demuth I, Wlodarski M, Tipping AJ, Morgan NV, de Winter JP, Thiel M, Gräsl S, Schindler D, D'Andrea AD, Altay C, et al: Spectrum of mutations in the Fanconi anaemia group G gene, FANCG/XRCC9. Eur J Hum Genet. 8:861–868. 2000. View Article : Google Scholar : PubMed/NCBI

45 

Gallmeier E, Calhoun ES, Rago C, Brody JR, Cunningham SC, Hucl T, Gorospe M, Kohli M, Lengauer C and Kern SE: Targeted disruption of FANCC and FANCG in human cancer provides a preclinical model for specific therapeutic options. Gastroenterology. 130:2145–2154. 2006. View Article : Google Scholar : PubMed/NCBI

46 

Levitus M, Waisfisz Q, Godthelp BC, de Vries Y, Hussain S, Wiegant WW, Elghalbzouri-Maghrani E, Steltenpool J, Rooimans MA, Pals G, et al: The DNA helicase BRIP1 is defective in Fanconi anemia complementation group J. Nat Genet. 37:934–935. 2005. View Article : Google Scholar : PubMed/NCBI

47 

Levran O, Attwooll C, Henry RT, Milton KL, Neveling K, Rio P, Batish SD, Kalb R, Velleuer E, Barral S, et al: The BRCA1-interacting helicase BRIP1 is deficient in Fanconi anemia. Nat Genet. 37:931–933. 2005. View Article : Google Scholar : PubMed/NCBI

48 

Litman R, Peng M, Jin Z, Zhang F, Zhang J, Powell S, Andreassen PR and Cantor SB: BACH1 is critical for homologous recombination and appears to be the Fanconi anemia gene product FANCJ. Cancer Cell. 8:255–265. 2005. View Article : Google Scholar : PubMed/NCBI

49 

Xie J, Peng M, Guillemette S, Quan S, Maniatis S, Wu Y, Venkatesh A, Shaffer SA, Brosh RM Jr and Cantor SB: FANCJ/BACH1 acetylation at lysine 1249 regulates the DNA damage response. PLoS Genet. 8:e10027862012. View Article : Google Scholar : PubMed/NCBI

50 

Cantor SB and Guillemette S: Hereditary breast cancer and the BRCA1-associated FANCJ/BACH1/BRIP1. Future Oncol. 7:253–261. 2011. View Article : Google Scholar : PubMed/NCBI

51 

Sobin LH and Fleming ID: TNM Classification of Malignant Tumors, fifth edition (1997). Union Internationale Contre le Cancer and the American Joint Committee on Cancer. Cancer. 80:1803–1804. 1997. View Article : Google Scholar : PubMed/NCBI

52 

Idbaih A, Carvalho Silva R, Crinière E, Marie Y, Carpentier C, Boisselier B, Taillibert S, Rousseau A, Mokhtari K, Ducray F, et al: Genomic changes in progression of low-grade gliomas. J Neurooncol. 90:133–140. 2008. View Article : Google Scholar : PubMed/NCBI

53 

van Zeeburg HJ, Snijders PJ, Pals G, Hermsen MA, Rooimans MA, Bagby G, Soulier J, Gluckman E, Wennerberg J, Leemans CR, et al: Generation and molecular characterization of head and neck squamous cell lines of fanconi anemia patients. Cancer Res. 65:1271–1276. 2005. View Article : Google Scholar : PubMed/NCBI

54 

Nagao T1, Sugano I, Ishida Y, Tajima Y, Munakata S, Asoh A, Yamazaki K, Muto H, Konno A, Kondo Y, et al: Primary large-cell neuroendocrine carcinoma of the parotid gland: immunohistochemical and molecular analysis of two cases. Mod Pathol. 13:554–561. 2000. View Article : Google Scholar : PubMed/NCBI

55 

Tarmin L, Yin J, Zhou X, Suzuki H, Jiang HY, Rhyu MG, Abraham JM, Krasna MJ, Cottrell J and Meltzer SJ: Frequent loss of heterozygosity on chromosome 9 in adenocarcinoma and squamous cell carcinoma of the esophagus. Cancer Res. 54:6094–6096. 1994.PubMed/NCBI

56 

Chen CH, Chang CJ, Yang WS, Chen CL and Fann CSJ: A genome-wide scan using tree-based association analysis for candidate loci related to fasting plasma glucose levels. BMC Genet. 4(Suppl 1): S652003. View Article : Google Scholar

57 

Powlesland RM, Charles AK, Malik KTA, Reynolds PA, Pires S, Boavida M and Brown KW: Loss of heterozygosity at 7p in Wilms' tumour development. Br J Cancer. 82:323–329. 2000. View Article : Google Scholar : PubMed/NCBI

58 

Orsetti B, Courjal F, Cuny M, Rodriguez C and Theillet C: 17q21–q25 aberrations in breast cancer: Combined allelotyping and CGH analysis reveals 5 regions of allelic imbalance among which two correspond to DNA amplification. Oncogene. 18:6262–6270. 1999. View Article : Google Scholar : PubMed/NCBI

59 

Indraccolo S, Tisato V, Agata S, Moserle L, Ferrari S, Callegaro M, Persano L, Palma MD, Scaini MC, Esposito G, et al: Establishment and characterization of xenografts and cancer cell cultures derived from BRCA1 −/− epithelial ovarian cancers. Eur J Cancer. 42:1475–1483. 2006. View Article : Google Scholar : PubMed/NCBI

60 

Canzian F, Salovaara R, Hemminki A, Kristo P, Chadwick RB, Aaltonen LA and de la Chapelle A: Semiautomated assessment of loss of heterozygosity and replication error in tumors. Cancer Res. 56:3331–3337. 1996.PubMed/NCBI

61 

Cawkwell L, Bell SM, Lewis FA, Dixon MF, Taylor GR and Quirke P: Rapid detection of allele loss in colorectal tumours using microsatellites and fluorescent DNA technology. Br J Cancer. 67:1262–1267. 1993. View Article : Google Scholar : PubMed/NCBI

62 

Hahn M, Wieland I, Koufaki ON, Görgens H, Sobottka SB, Schackert G and Schackert HK: Genetic alterations of the tumor suppressor gene PTEN/MMAC1 in human brain metastases. Clin Cancer Res. 5:2431–2437. 1999.PubMed/NCBI

63 

Ihaka R and Gentleman R: R: A language for data analysis and graphics. J Comput Graph Stat. 5:299–314. 1996.

64 

Bland JM and Altman DG: Multiple significance tests: The Bonferroni method. BMJ. 310:1701995. View Article : Google Scholar : PubMed/NCBI

65 

Cheng PF, Dummer R and Levesque MP: Data mining The Cancer Genome Atlas in the era of precision cancer medicine. Swiss Med Wkly. 145:w141832015.PubMed/NCBI

66 

Subramanian A, Tamayo P, Mootha VK, Mukherjee S, Ebert BL, Gillette MA, Paulovich A, Pomeroy SL, Golub TR, Lander ES, et al: Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles. Proc Natl Acad Sci USA. 102:15545–15550. 2005. View Article : Google Scholar : PubMed/NCBI

67 

Warde-Farley D, Donaldson SL, Comes O, Zuberi K, Badrawi R, Chao P, Franz M, Grouios C, Kazi F, Lopes CT, et al: The GeneMANIA prediction server: Biological network integration for gene prioritization and predicting gene function. Nucleic Acids Res. 38(Web Server): W214–W220. 2010. View Article : Google Scholar : PubMed/NCBI

68 

Kuriakose MA, Chen WT, He ZM, Sikora AG, Zhang P, Zhang ZY, Qiu WL, Hsu DF, McMunn-Coffran C, Brown SM, et al: Selection and validation of differentially expressed genes in head and neck cancer. Cell Mol Life Sci. 61:1372–1383. 2004. View Article : Google Scholar : PubMed/NCBI

69 

Hu N, Clifford RJ, Yang HH, Wang C, Goldstein AM, Ding T, Taylor PR and Lee MP: Genome wide analysis of DNA copy number neutral loss of heterozygosity (CNNLOH) and its relation to gene expression in esophageal squamous cell carcinoma. BMC Genomics. 11:5762010. View Article : Google Scholar : PubMed/NCBI

70 

Zhu B, Yan K, Li L, Lin M, Zhang S, He Q, Zheng D, Yang H and Shao G: K63-linked ubiquitination of FANCG is required for its association with the Rap80-BRCA1 complex to modulate homologous recombination repair of DNA interstand crosslinks. Oncogene. 34:2867–2878. 2015. View Article : Google Scholar

71 

Forbes SA, Bindal N, Bamford S, Cole C, Kok CY, Beare D, Jia M, Shepherd R, Leung K, Menzies A, et al: COSMIC: Mining complete cancer genomes in the Catalogue of Somatic Mutations in Cancer. Nucleic Acids Res. 39(Database): D945–D950. 2011. View Article : Google Scholar :

72 

Deans AJ and West SC: DNA interstrand crosslink repair and cancer. Nat Rev Cancer. 11:467–480. 2011. View Article : Google Scholar : PubMed/NCBI

73 

Ridet A, Guillouf C, Duchaud E, Cundari E, Fiore M, Moustacchi E and Rosselli F: Deregulated apoptosis is a hallmark of the Fanconi anemia syndrome. Cancer Res. 57:1722–1730. 1997.PubMed/NCBI

74 

Beder LB, Gunduz M, Ouchida M, Fukushima K, Gunduz E, Ito S, Sakai A, Nagai N, Nishizaki K and Shimizu K: Genome-wide analyses on loss of heterozygosity in head and neck squamous cell carcinomas. Lab Invest. 83:99–105. 2003. View Article : Google Scholar : PubMed/NCBI

75 

McHugh PJ, Spanswick VJ and Hartley JA: Repair of DNA inter-strand crosslinks: Molecular mechanisms and clinical relevance. Lancet Oncol. 2:483–490. 2001. View Article : Google Scholar

76 

Bridge WL, Vandenberg CJ, Franklin RJ and Hiom K: The BRIP1 helicase functions independently of BRCA1 in the Fanconi anemia pathway for DNA crosslink repair. Nat Genet. 37:953–957. 2005. View Article : Google Scholar : PubMed/NCBI

77 

Hoffmann M, Görögh T, Gottschlich S, Lohrey C, Rittgen W, Ambrosch P, Schwarz E and Kahn T: Human papillomaviruses in head and neck cancer: 8 year-survival-analysis of 73 patients. Cancer Lett. 218:199–206. 2005. View Article : Google Scholar : PubMed/NCBI

78 

Sticht C, Hofele C, Flechtenmacher C, Bosch FX, Freier K, Lichter P and Joos S: Amplification of Cyclin L1 is associated with lymph node metastases in head and neck squamous cell carcinoma (HNSCC). Br J Cancer. 92:770–774. 2005. View Article : Google Scholar : PubMed/NCBI

79 

Hashemi R and Commenges D: Correction of the p-value after multiple tests in a Cox proportional hazard model. Lifetime Data Anal. 8:335–348. 2002. View Article : Google Scholar : PubMed/NCBI

80 

Bryne M, Koppang HS, Lilleng R, Stene T, Bang G and Dabelsteen E: New malignancy grading is a better prognostic indicator than Broders' grading in oral squamous cell carcinomas. J Oral Pathol Med. 18:432–437. 1989. View Article : Google Scholar : PubMed/NCBI

81 

Kristensen GB, Abeler VM, Risberg B, Trop C and Bryne M: Tumor size, depth of invasion, and grading of the invasive tumor front are the main prognostic factors in early squamous cell cervical carcinoma. Gynecol Oncol. 74:245–251. 1999. View Article : Google Scholar : PubMed/NCBI

82 

Bryne M, Koppang HS, Lilleng R and Kjaerheim A: Malignancy grading of the deep invasive margins of oral squamous cell carcinomas has high prognostic value. J Pathol. 166:375–381. 1992. View Article : Google Scholar : PubMed/NCBI

83 

Lim SL, Smith P, Syed N, Coens C, Wong H, van der Burg M, Szlosarek P, Crook T and Green JA: Promoter hypermethylation of FANCF and outcome in advanced ovarian cancer. Br J Cancer. 98:1452–1456. 2008. View Article : Google Scholar : PubMed/NCBI

84 

Begg AC: Predicting recurrence after radiotherapy in head and neck cancer. Semin Radiat Oncol. 22:108–118. 2012. View Article : Google Scholar : PubMed/NCBI

85 

Curtin NJ and Szabo C: Therapeutic applications of PARP inhibitors: Anticancer therapy and beyond. Mol Aspects Med. 34:1217–1256. 2013. View Article : Google Scholar : PubMed/NCBI

86 

McCabe N, Turner NC, Lord CJ, Kluzek K, Bialkowska A, Swift S, Giavara S, O'Connor MJ, Tutt AN, Zdzienicka MZ, et al: Deficiency in the repair of DNA damage by homologous recombination and sensitivity to poly(ADP-ribose) polymerase inhibition. Cancer Res. 66:8109–8115. 2006. View Article : Google Scholar : PubMed/NCBI

87 

Nowsheen S, Bonner JA and Yang ES: The poly(ADP-Ribose) polymerase inhibitor ABT-888 reduces radiation-induced nuclear EGFR and augments head and neck tumor response to radiotherapy. Radiother Oncol. 99:331–338. 2011. View Article : Google Scholar : PubMed/NCBI

88 

Khan K, Araki K, Wang D, Li G, Li X, Zhang J, Xu W, Hoover RK, Lauter S, O'Malley B Jr, et al: Head and neck cancer radiosensitization by the novel poly(ADP-ribose) polymerase inhibitor GPI-15427. Head Neck. 32:381–391. 2010.

89 

Li Y, Zhao L, Sun H, Yu J, Li N, Liang J, Wang Y, He M, Bai X, Yu Z, et al: Gene silencing of FANCF potentiates the sensitivity to mitoxantrone through activation of JNK and p38 signal pathways in breast cancer cells. PLoS One. 7:e442542012. View Article : Google Scholar : PubMed/NCBI

90 

Yu J, Zhao L, Li Y, Li N, He M, Bai X, Yu Z, Zheng Z, Mi X, Wang E, et al: Silencing of fanconi anemia complementation group f exhibits potent chemosensitization of mitomycin C activity in breast cancer cells. J Breast Cancer. 16:291–299. 2013. View Article : Google Scholar : PubMed/NCBI

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June-2017
Volume 50 Issue 6

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Copy and paste a formatted citation
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Spandidos Publications style
Türke C, Horn S, Petto C, Labudde D, Lauer G and Wittenburg G: Loss of heterozygosity in FANCG, FANCF and BRIP1 from head and neck squamous cell carcinoma of the oral cavity. Int J Oncol 50: 2207-2220, 2017
APA
Türke, C., Horn, S., Petto, C., Labudde, D., Lauer, G., & Wittenburg, G. (2017). Loss of heterozygosity in FANCG, FANCF and BRIP1 from head and neck squamous cell carcinoma of the oral cavity. International Journal of Oncology, 50, 2207-2220. https://doi.org/10.3892/ijo.2017.3974
MLA
Türke, C., Horn, S., Petto, C., Labudde, D., Lauer, G., Wittenburg, G."Loss of heterozygosity in FANCG, FANCF and BRIP1 from head and neck squamous cell carcinoma of the oral cavity". International Journal of Oncology 50.6 (2017): 2207-2220.
Chicago
Türke, C., Horn, S., Petto, C., Labudde, D., Lauer, G., Wittenburg, G."Loss of heterozygosity in FANCG, FANCF and BRIP1 from head and neck squamous cell carcinoma of the oral cavity". International Journal of Oncology 50, no. 6 (2017): 2207-2220. https://doi.org/10.3892/ijo.2017.3974