Secretomic profiling of cells from hollow fiber bioreactor reveals PSMA3 as a potential cholangiocarcinoma biomarker

  • Authors:
    • Chris Verathamjamras
    • Churat Weeraphan
    • Daranee Chokchaichamnankit
    • Kamolwan Watcharatanyatip
    • Pantipa Subhasitanont
    • Penchatr Diskul-Na-Ayudthaya
    • Kanokwan Mingkwan
    • Virat Luevisadpaibul
    • Somchai Chutipongtanate
    • Voraratt Champattanachai
    • Jisnuson Svasti
    • Chantragan Srisomsap
  • View Affiliations

  • Published online on: May 29, 2017     https://doi.org/10.3892/ijo.2017.4024
  • Pages: 269-280
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Abstract

Cholangiocarcinoma (CCA), derived from the bile duct, occurs with a relatively high incidence in Northeast Thailand. Early diagnosis is still hampered by the lack of sufficient biomarkers. In recent years, biomarker discovery using secretomes has provided interesting results, including our studies on CCA secretomes, especially with three-dimensional cell cultures. Thus, cells cultured using the hollow fiber bioreactor (HFB) with 20 kDa molecular weight cut-off (MWCO) yielded higher quality and quantity of secretomes than those from conditioned media of the monolayer culture (MNC) system. In this study, we employed the HFB culture system with 5 kDa MWCO and compared conditioned media from the HFB and MNC systems using two-dimensional gel electrophoresis, followed by identifying proteins of interest by liquid chromatography and mass spectrometry (LC/MS/MS). Two out of 4 spots of NGAL or lipocalin-2 were found to show highest increase in expression of 19.93-fold and 18.79-fold in HFB compared to MNC. Interestingly, all 14 proteasome subunits including proteasome subunit α type-1 to type-7 and β type-1 to type-7 showed 2.92-fold to 12.13-fold increased expression in HFB. The protein-protein interactions of upregulated proteins were predicted, and one of the main interaction clusters involved 20S proteasome subunits. Proteasome activity in the HFB conditioned media was also found to be higher than that in MNC conditioned media. Three types of proteasome subunit were also validated by immunoblotting and showed higher expression in the HFB system compared to MNC system. Proteasome subunit α type-3 (PSMA3) showed the highest level in plasma of cholangiocarcinoma patients compared to normal and hepatocellular carcinoma patients by immunodetection, and is of interest as a potential biomarker for cholangiocarcinoma.
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July-2017
Volume 51 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Verathamjamras C, Weeraphan C, Chokchaichamnankit D, Watcharatanyatip K, Subhasitanont P, Diskul-Na-Ayudthaya P, Mingkwan K, Luevisadpaibul V, Chutipongtanate S, Champattanachai V, Champattanachai V, et al: Secretomic profiling of cells from hollow fiber bioreactor reveals PSMA3 as a potential cholangiocarcinoma biomarker. Int J Oncol 51: 269-280, 2017
APA
Verathamjamras, C., Weeraphan, C., Chokchaichamnankit, D., Watcharatanyatip, K., Subhasitanont, P., Diskul-Na-Ayudthaya, P. ... Srisomsap, C. (2017). Secretomic profiling of cells from hollow fiber bioreactor reveals PSMA3 as a potential cholangiocarcinoma biomarker. International Journal of Oncology, 51, 269-280. https://doi.org/10.3892/ijo.2017.4024
MLA
Verathamjamras, C., Weeraphan, C., Chokchaichamnankit, D., Watcharatanyatip, K., Subhasitanont, P., Diskul-Na-Ayudthaya, P., Mingkwan, K., Luevisadpaibul, V., Chutipongtanate, S., Champattanachai, V., Svasti, J., Srisomsap, C."Secretomic profiling of cells from hollow fiber bioreactor reveals PSMA3 as a potential cholangiocarcinoma biomarker". International Journal of Oncology 51.1 (2017): 269-280.
Chicago
Verathamjamras, C., Weeraphan, C., Chokchaichamnankit, D., Watcharatanyatip, K., Subhasitanont, P., Diskul-Na-Ayudthaya, P., Mingkwan, K., Luevisadpaibul, V., Chutipongtanate, S., Champattanachai, V., Svasti, J., Srisomsap, C."Secretomic profiling of cells from hollow fiber bioreactor reveals PSMA3 as a potential cholangiocarcinoma biomarker". International Journal of Oncology 51, no. 1 (2017): 269-280. https://doi.org/10.3892/ijo.2017.4024