TAZ overexpression is associated with epithelial-mesenchymal transition in cisplatin-resistant gastric cancer cells

  • Authors:
    • Liang Ge
    • Dong-Song Li
    • Fei Chen
    • Ji-Dong Feng
    • Bai Li
    • Tie-Jun Wang
  • View Affiliations

  • Published online on: May 16, 2017     https://doi.org/10.3892/ijo.2017.3998
  • Pages: 307-315
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Abstract

Gastric cancer is one of the common malignant diseases. The poor treatment outcome is mainly due to chemotherapeutic resistance. Therefore, it is important to determine the molecular mechanism of drug resistance in gastric cancer. To explore the mechanisms of cisplatin resistance in gastric cancer cells, several approaches were performed including MTT assay, real-time RT-PCR, western blot analysis, migration and invasion assays, wound healing assay, and transfection. We found that cisplatin-resistant (CR) gastric cancer cells acquired epithelial-mesenchymal transition (EMT) phenotype. The CR cells with EMT features obtained higher migratory and invasive activities. Moreover, we observed that TAZ was highly expressed in CR cells. Consistently, depletion of TAZ caused partial reversal of EMT to MET in CR cells. Our results suggest that TAZ plays a pivotal role in CR-induced EMT. Targeting TAZ could be a potential therapeutic strategy for gastric cancer.
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July-2017
Volume 51 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Ge L, Li D, Chen F, Feng J, Li B and Wang T: TAZ overexpression is associated with epithelial-mesenchymal transition in cisplatin-resistant gastric cancer cells. Int J Oncol 51: 307-315, 2017
APA
Ge, L., Li, D., Chen, F., Feng, J., Li, B., & Wang, T. (2017). TAZ overexpression is associated with epithelial-mesenchymal transition in cisplatin-resistant gastric cancer cells. International Journal of Oncology, 51, 307-315. https://doi.org/10.3892/ijo.2017.3998
MLA
Ge, L., Li, D., Chen, F., Feng, J., Li, B., Wang, T."TAZ overexpression is associated with epithelial-mesenchymal transition in cisplatin-resistant gastric cancer cells". International Journal of Oncology 51.1 (2017): 307-315.
Chicago
Ge, L., Li, D., Chen, F., Feng, J., Li, B., Wang, T."TAZ overexpression is associated with epithelial-mesenchymal transition in cisplatin-resistant gastric cancer cells". International Journal of Oncology 51, no. 1 (2017): 307-315. https://doi.org/10.3892/ijo.2017.3998