Open Access

Disruption of serine/threonine protein phosphatase 5 inhibits tumorigenesis of urinary bladder cancer cells

  • Authors:
    • Ming Chen
    • Jian-Min Lv
    • Jian-Qing Ye
    • Xin-Gang Cui
    • Fa-Jun Qu
    • Lu Chen
    • Xi Liu
    • Xiu-Wu Pan
    • Lin Li
    • Hai Huang
    • Qi-Wei Yang
    • Jie Chen
    • Lin-Hui Wang
    • Yi Gao
    • Dan-Feng Xu
  • View Affiliations

  • Published online on: May 16, 2017     https://doi.org/10.3892/ijo.2017.3997
  • Pages: 39-48
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Serine/threonine protein phosphatase 5 (PPP5C) is a member of the protein serine/threonine phosphatase family and has been shown to participate in multiple signaling cascades and tumor progression. We found that PPP5C was highly expressed in bladder cancer tissues compared to normal urothelial tissues, and positively correlated to tumor stages through ONCOMINE microarray data mining. Knockdown of PPP5C via a lentivirus-mediated short hairpin RNA (shRNA) markedly inhibited cell proliferation and colony formation. Flow cytometric analysis showed that PPP5C-deficient T24 and BT5637 bladder cancer cells were arrested in G0/G1 phase and induced apoptosis. In addition, tumor growth was inhibited in vivo in a xenograft nude mouse model. Further studies indicated that knockdown of PPP5C downregulated c-myc and CDK4, whereas upregulated p27, BAD and Beclin1. These results suggest that PPP5C is associated with bladder cancer (BCa) and plays an oncogenic role in the development and progression of bladder cancer.
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July-2017
Volume 51 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
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Spandidos Publications style
Chen M, Lv J, Ye J, Cui X, Qu F, Chen L, Liu X, Pan X, Li L, Huang H, Huang H, et al: Disruption of serine/threonine protein phosphatase 5 inhibits tumorigenesis of urinary bladder cancer cells. Int J Oncol 51: 39-48, 2017
APA
Chen, M., Lv, J., Ye, J., Cui, X., Qu, F., Chen, L. ... Xu, D. (2017). Disruption of serine/threonine protein phosphatase 5 inhibits tumorigenesis of urinary bladder cancer cells. International Journal of Oncology, 51, 39-48. https://doi.org/10.3892/ijo.2017.3997
MLA
Chen, M., Lv, J., Ye, J., Cui, X., Qu, F., Chen, L., Liu, X., Pan, X., Li, L., Huang, H., Yang, Q., Chen, J., Wang, L., Gao, Y., Xu, D."Disruption of serine/threonine protein phosphatase 5 inhibits tumorigenesis of urinary bladder cancer cells". International Journal of Oncology 51.1 (2017): 39-48.
Chicago
Chen, M., Lv, J., Ye, J., Cui, X., Qu, F., Chen, L., Liu, X., Pan, X., Li, L., Huang, H., Yang, Q., Chen, J., Wang, L., Gao, Y., Xu, D."Disruption of serine/threonine protein phosphatase 5 inhibits tumorigenesis of urinary bladder cancer cells". International Journal of Oncology 51, no. 1 (2017): 39-48. https://doi.org/10.3892/ijo.2017.3997