Molecular analysis of keratocystic odontogenic tumor cell lines derived from sporadic and basal cell nevus syndrome patients

  • Authors:
    • Kazuma Noguchi
    • Keiko Wakai
    • Tohru Kiyono
    • Mutsuki Kawabe
    • Kyohei Yoshikawa
    • Tomoko Hashimoto-Tamaoki
    • Hiromitsu Kishimoto
    • Yoshiro Nakano
  • View Affiliations

  • Published online on: October 9, 2017     https://doi.org/10.3892/ijo.2017.4146
  • Pages:1731-1738
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Abstract

Keratocystic odontogenic tumor (KCOT) is a benign tumor often associated with basal cell nevus syndrome (BCNS). Mutations in Patched 1 (PTCH1), the Hedgehog (Hh) receptor, are responsible for BCNS. BCNS is distinguished by morphological anomalies and predisposition to benign and malignant tumors, including medulloblastoma, basal cell carcinoma, KCOT and ovarian fibromas. Among these tumors, KCOT is the least well studied because a suitable model system is not available for its investigation. To enable KCOT to be studied, we established two KCOT cell lines, one from a BCNS case (designated as iKCOT1) and one from a sporadic KCOT case (designated as sKCOT1). The BCNS‑derived KCOT cell line, iKCOT1, retained a germline-mutated PTCH1 allele and a wild-type PTCH1 allele. The sporadic KCOT-derived KCOT cell line, sKCOT1, had different loss-of-function PTCH1 mutations on both alleles. Both cell lines expressed stem cell markers (CD44, SOX2 and BMI1), mesenchymal cell markers (CDH2, VIM and SNAI2) and a neurogenic marker (NEFL). Culture of the cell lines in high calcium concentration media induced expression of epithelial cell and keratinocyte marker proteins (CDH1, CLDN1, KRT10 and IVL). Parakeratosis, which is characteristic for KCOTs, was observed in 2-D cultures. The similarities in protein expression patterns between the two cell lines suggested that common mechanisms underlie the development of both types of KCOT and a probable common origin of KCOT cells.

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December 2017
Volume 51 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

2016 Impact Factor: 3.079
Ranked #33/217 Oncology
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APA
Noguchi, K., Wakai, K., Kiyono, T., Kawabe, M., Yoshikawa, K., Hashimoto-Tamaoki, T. ... Nakano, Y. (2017). Molecular analysis of keratocystic odontogenic tumor cell lines derived from sporadic and basal cell nevus syndrome patients. International Journal of Oncology, 51, 1731-1738. https://doi.org/10.3892/ijo.2017.4146
MLA
Noguchi, K., Wakai, K., Kiyono, T., Kawabe, M., Yoshikawa, K., Hashimoto-Tamaoki, T., Kishimoto, H., Nakano, Y."Molecular analysis of keratocystic odontogenic tumor cell lines derived from sporadic and basal cell nevus syndrome patients". International Journal of Oncology 51.6 (2017): 1731-1738.
Chicago
Noguchi, K., Wakai, K., Kiyono, T., Kawabe, M., Yoshikawa, K., Hashimoto-Tamaoki, T., Kishimoto, H., Nakano, Y."Molecular analysis of keratocystic odontogenic tumor cell lines derived from sporadic and basal cell nevus syndrome patients". International Journal of Oncology 51, no. 6 (2017): 1731-1738. https://doi.org/10.3892/ijo.2017.4146