Knockdown of GA-binding protein subunit β1 inhibits cell proliferation via p21 induction in renal cell carcinoma

  • Authors:
    • Szu-Chia Chen
    • Meng-Chi Yen
    • Feng-Wei Chen
    • Ling-Yu Wu
    • Shiang-Jie Yang
    • Po-Lin Kuo
    • Ya-Ling Hsu
  • View Affiliations

  • Published online on: May 17, 2018     https://doi.org/10.3892/ijo.2018.4411
  • Pages: 886-894
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Abstract

Clear cell renal cell carcinoma (ccRCC) is the most common type of renal cancer. In the present study, bioinformatics tools were systematically used to investigate the potential upstream effector involved in the progression of ccRCC. Using the Gene Expression Omnibus database and Library of Integrated Network-based Cellular Signatures L1000 platform, it was identified that GA-binding protein subunit β1 (GABPB1) was a potential effector gene. GABPB1 is a transcription factor subunit and its function in ccRCC is unclear. Elevated expression of GABPB1 mRNA in ccRCC was also observed in other clinical datasets from the Oncomine database. Following reverse transcription-quantitative polymerase chain reaction and western blot analysis, the ccRCC 786-O and A498 cell lines showed higher expression levels of GABPB1 than HK-2, a normal kidney cell line. Knockdown of GABPB1 in the 786-O and A498 cells significantly decreased the ability to form colonies by inducing the expression of p21Waf/Cip1. SurvExpress database analysis indicated that a higher expression of GABPB1 was associated with poor survival outcome in patients with renal cancer. These findings imply that GABPB1 serves an important role in the progression of ccRCC.
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August-2018
Volume 53 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Chen S, Yen M, Chen F, Wu L, Yang S, Kuo P and Hsu Y: Knockdown of GA-binding protein subunit β1 inhibits cell proliferation via p21 induction in renal cell carcinoma. Int J Oncol 53: 886-894, 2018
APA
Chen, S., Yen, M., Chen, F., Wu, L., Yang, S., Kuo, P., & Hsu, Y. (2018). Knockdown of GA-binding protein subunit β1 inhibits cell proliferation via p21 induction in renal cell carcinoma. International Journal of Oncology, 53, 886-894. https://doi.org/10.3892/ijo.2018.4411
MLA
Chen, S., Yen, M., Chen, F., Wu, L., Yang, S., Kuo, P., Hsu, Y."Knockdown of GA-binding protein subunit β1 inhibits cell proliferation via p21 induction in renal cell carcinoma". International Journal of Oncology 53.2 (2018): 886-894.
Chicago
Chen, S., Yen, M., Chen, F., Wu, L., Yang, S., Kuo, P., Hsu, Y."Knockdown of GA-binding protein subunit β1 inhibits cell proliferation via p21 induction in renal cell carcinoma". International Journal of Oncology 53, no. 2 (2018): 886-894. https://doi.org/10.3892/ijo.2018.4411