Open Access

Loss of profilin 2 contributes to enhanced epithelial-mesenchymal transition and metastasis of colorectal cancer

  • Authors:
    • Hui Zhang
    • Weiqiang Yang
    • Jinlong Yan
    • Kaiping Zhou
    • Boshun Wan
    • Peidong Shi
    • Yueyu Chen
    • Songbing He
    • Dechun Li
  • View Affiliations

  • Published online on: July 9, 2018     https://doi.org/10.3892/ijo.2018.4475
  • Pages: 1118-1128
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Profilin 2 (PFN2) functions as an actin cytoskeleton regulator and serves an important role in cell motility. However, a role for PFN2 in the progression of colorectal cancer (CRC), particularly in metastasis, has yet to be clarified. The aim of the present study was to investigate whether PFN2 served specific roles in the progression of human CRC. The results demonstrated that PFN2 was differentially expressed in CRC tissues and cell lines by reverse transcription-quantitative polymerase chain reaction and western blotting. PFN2 expression was also negatively associated with the degree of tumor metastasis. Low PFN2 expression in CRC cells was related with enhanced epithelial-mesenchymal transition (EMT) and, in turn, may increase migratory capabilities. Overexpression of PFN2 in CRC cell lines with a low level of endogenous PFN2 inhibited the EMT process, as well as the associated migration; in addition, myosin light chain (MLC) phosphorylation was upregulated. Inhibition of MLC phosphorylation attenuated the inhibition of EMT and cell migratory abilities induced by PFN2 overexpression in CRC cell lines, the results suggested that PFN2 may suppress cancer EMT and the subsequent metastasis by regulating cytoskeletal reorganization. These results demonstrated that PFN2 may serve a suppressive role in the metastasis of CRC and therefore may provide a new potential target for cancer therapeutics.
View Figures
View References

Related Articles

Journal Cover

September-2018
Volume 53 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhang H, Yang W, Yan J, Zhou K, Wan B, Shi P, Chen Y, He S and Li D: Loss of profilin 2 contributes to enhanced epithelial-mesenchymal transition and metastasis of colorectal cancer. Int J Oncol 53: 1118-1128, 2018
APA
Zhang, H., Yang, W., Yan, J., Zhou, K., Wan, B., Shi, P. ... Li, D. (2018). Loss of profilin 2 contributes to enhanced epithelial-mesenchymal transition and metastasis of colorectal cancer. International Journal of Oncology, 53, 1118-1128. https://doi.org/10.3892/ijo.2018.4475
MLA
Zhang, H., Yang, W., Yan, J., Zhou, K., Wan, B., Shi, P., Chen, Y., He, S., Li, D."Loss of profilin 2 contributes to enhanced epithelial-mesenchymal transition and metastasis of colorectal cancer". International Journal of Oncology 53.3 (2018): 1118-1128.
Chicago
Zhang, H., Yang, W., Yan, J., Zhou, K., Wan, B., Shi, P., Chen, Y., He, S., Li, D."Loss of profilin 2 contributes to enhanced epithelial-mesenchymal transition and metastasis of colorectal cancer". International Journal of Oncology 53, no. 3 (2018): 1118-1128. https://doi.org/10.3892/ijo.2018.4475