Open Access

MYB promotes the growth and metastasis of salivary adenoid cystic carcinoma

  • Authors:
    • Li‑Hua Xu
    • Fei Zhao
    • Wen‑Wen Yang
    • Chu‑Wen Chen
    • Zhi‑Hao Du
    • Min Fu
    • Xi‑Yuan Ge
    • Sheng‑Lin Li
  • View Affiliations

  • Published online on: March 18, 2019     https://doi.org/10.3892/ijo.2019.4754
  • Pages: 1579-1590
  • Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The incidence of recurrent t(6;9) translocation of the MYB proto‑oncogene to NFIB (the gene that encodes nuclear factor 1 B‑type) in adenoid cystic carcinoma (ACC) tumour tissues is high. However, MYB [the gene that encodes transcriptional activator Myb (MYB)] overexpression is more common, indicating that MYB serves a key role in ACC. The current study aimed to investigate the role of MYB in salivary (S)ACC growth and metastasis. A total of 50 fresh‑frozen SACC tissues and 41 fresh‑frozen normal submandibular gland (SMG) tissues were collected to measure MYB mRNA expression, and to analyse the associations between MYB and epithelial‑mesenchymal transition (EMT) markers. Compared with normal SMG tissue, SACC tissues demonstrated significantly increased MYB expression, with a high expression rate of 90%. Interestingly, MYB tended to be negatively correlated with CDH1 [the gene that encodes cadherin‑1 (E‑cadherin)] and positively correlated with VIM (the gene that encodes vimentin), suggesting that MYB is associated with SACC metastasis. To explore the role of MYB in SACC, the authors stably overexpressed and knocked down MYB in SACC cells. The authors of the current study demonstrated that MYB overexpression promoted SACC cell proliferation, migration and invasion, whereas its knockdown inhibited these activities. Additionally, when MYB was overexpressed, CDH1 expression was downregulated, and CDH2 (the gene that encodes cadherin‑2), VIM and ACTA2 (the gene that encodes actin, aortic smooth muscle) expression was upregulated. Then, the effect of MYB on lung tumour metastasis was investigated in vivo in non‑obese diabetic/severe combined immunodeficiency mice. MYB overexpressing and control cells were injected into the mice through the tail vein. The results revealed that MYB promoted SACC lung metastasis. Collectively, these results demonstrated that MYB is aberrantly overexpressed in SACC tissues, and promotes SACC cell proliferation and metastasis, indicating that MYB may be a novel therapeutic target for SACC.
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May-2019
Volume 54 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
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Spandidos Publications style
Xu LH, Zhao F, Yang WW, Chen CW, Du ZH, Fu M, Ge XY and Li SL: MYB promotes the growth and metastasis of salivary adenoid cystic carcinoma. Int J Oncol 54: 1579-1590, 2019
APA
Xu, L., Zhao, F., Yang, W., Chen, C., Du, Z., Fu, M. ... Li, S. (2019). MYB promotes the growth and metastasis of salivary adenoid cystic carcinoma. International Journal of Oncology, 54, 1579-1590. https://doi.org/10.3892/ijo.2019.4754
MLA
Xu, L., Zhao, F., Yang, W., Chen, C., Du, Z., Fu, M., Ge, X., Li, S."MYB promotes the growth and metastasis of salivary adenoid cystic carcinoma". International Journal of Oncology 54.5 (2019): 1579-1590.
Chicago
Xu, L., Zhao, F., Yang, W., Chen, C., Du, Z., Fu, M., Ge, X., Li, S."MYB promotes the growth and metastasis of salivary adenoid cystic carcinoma". International Journal of Oncology 54, no. 5 (2019): 1579-1590. https://doi.org/10.3892/ijo.2019.4754