TRANSCRIPTIONAL ANTAGONISM BETWEEN P53 AND SV40 T-ANTIGEN

  • Authors:
    • S DEB
    • DR BROWN
    • HM LEGA
    • SP DEB
  • View Affiliations

  • Published online on: January 1, 1995     https://doi.org/10.3892/ijo.6.1.243
  • Pages: 243-249
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Abstract

In this communication we show that overexpression of the human TATA-binding protein (TBP) activates a synthetic promoter with p53-binding sites in the presence of wild-type p53. No activation is observed in the absence of wild-type p53 in Saos-2 cells. Perhaps, TBP is limiting in these cells for the p53-mediated activation, and p53 activates promoters with p53-binding sites via its interaction with TBP. Using in vivo transient transfection-transcription assays, we also show that wild-type human p53 inhibits simian virus 40 (SV40) late promoter transactivation by SV40 T antigen, but excess T antigen partially releases this inhibition. Recently both T antigen and p53 have been shown to interact with TBP. The inhibition of T antigen-mediated SV40 late promoter activation by p53 or the inhibition of p53-mediated activation of a promoter with p53-binding sites by T antigen is not released by an overexpression of TBP. Thus, the transcriptional antagonism between p53 and T antigen are not at the level of competition for TBP. Possibly, the two proteins poison each other so that they cannot interact with the transcription machinery.

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January 1995
Volume 6 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
DEB S, BROWN D, LEGA H and DEB S: TRANSCRIPTIONAL ANTAGONISM BETWEEN P53 AND SV40 T-ANTIGEN. Int J Oncol 6: 243-249, 1995
APA
DEB, S., BROWN, D., LEGA, H., & DEB, S. (1995). TRANSCRIPTIONAL ANTAGONISM BETWEEN P53 AND SV40 T-ANTIGEN. International Journal of Oncology, 6, 243-249. https://doi.org/10.3892/ijo.6.1.243
MLA
DEB, S., BROWN, D., LEGA, H., DEB, S."TRANSCRIPTIONAL ANTAGONISM BETWEEN P53 AND SV40 T-ANTIGEN". International Journal of Oncology 6.1 (1995): 243-249.
Chicago
DEB, S., BROWN, D., LEGA, H., DEB, S."TRANSCRIPTIONAL ANTAGONISM BETWEEN P53 AND SV40 T-ANTIGEN". International Journal of Oncology 6, no. 1 (1995): 243-249. https://doi.org/10.3892/ijo.6.1.243