C-HA-RAS ENHANCES THE NEOPLASTIC TRANSFORMATION OF HUMAN BREAST EPITHELIAL-CELLS TREATED WITH CHEMICAL CARCINOGENS

  • Authors:
    • G CALAF
    • PL ZHANG
    • MV ALVARADO
    • S ESTRADA
    • J RUSSO
  • View Affiliations

  • Published online on: January 1, 1995     https://doi.org/10.3892/ijo.6.1.5
  • Pages: 5-11
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Abstract

The present study was carried out with the purpose of analyzing the additive effect of c-Ha-ras oncogene on tumorigenesis in human breast epithelial cells (HBEC) treated with chemical carcinogens. A human breast epithelial cell (HBEC) line, MCF-10F, previously treated with dimethylbenz(a) anthracene (DMBA) and benzo(a)pyrene (BP) was used in these studies. The MCF-10F cells, DMBA and/or BP-transformed cells originated from the clones D3-1 and BP1 which were transfected with the plasmid pH06T1 containing the human T24 mutated c-Ha-ras oncogene and termed MCF-10F-Tras, D3-1-Tras and BP1-Tras, respectively. Whereas the c-Ha-ras transfected cells presented altered morphology, increased anchorage independent growth in agar-methocel, invasiveness and tumorigenicity, the MCF-10F cells, the clones D3 and BP1 were not tumorigenic. Importantly, whereas MCF-10F-Tras was slightly tumorigenic, the D3-1-Tras and BP1-Tras transfected cells were 100% tumorigenic in the SCID mice; and the tumors thus obtained were poorly differentiated carcinomas. DNA fingerprinting confirmed that the tumors derived originated from the cell lineage used. It was concluded that c-Ha ras induces an additive effect on the expression of tumorigenesis in human breast epithelial cell line MCF-10F treated with chemical carcinogens. Our work provide a model for analyzing the role of c-Ha-ras in human breast cancer.

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January 1995
Volume 6 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
CALAF G, ZHANG P, ALVARADO M, ESTRADA S and RUSSO J: C-HA-RAS ENHANCES THE NEOPLASTIC TRANSFORMATION OF HUMAN BREAST EPITHELIAL-CELLS TREATED WITH CHEMICAL CARCINOGENS. Int J Oncol 6: 5-11, 1995
APA
CALAF, G., ZHANG, P., ALVARADO, M., ESTRADA, S., & RUSSO, J. (1995). C-HA-RAS ENHANCES THE NEOPLASTIC TRANSFORMATION OF HUMAN BREAST EPITHELIAL-CELLS TREATED WITH CHEMICAL CARCINOGENS. International Journal of Oncology, 6, 5-11. https://doi.org/10.3892/ijo.6.1.5
MLA
CALAF, G., ZHANG, P., ALVARADO, M., ESTRADA, S., RUSSO, J."C-HA-RAS ENHANCES THE NEOPLASTIC TRANSFORMATION OF HUMAN BREAST EPITHELIAL-CELLS TREATED WITH CHEMICAL CARCINOGENS". International Journal of Oncology 6.1 (1995): 5-11.
Chicago
CALAF, G., ZHANG, P., ALVARADO, M., ESTRADA, S., RUSSO, J."C-HA-RAS ENHANCES THE NEOPLASTIC TRANSFORMATION OF HUMAN BREAST EPITHELIAL-CELLS TREATED WITH CHEMICAL CARCINOGENS". International Journal of Oncology 6, no. 1 (1995): 5-11. https://doi.org/10.3892/ijo.6.1.5