ABSENCE OF STIMULATORY EFFECT OF G-CSF ON THE GROWTH OF HUMAN SARCOMA-CELLS

  • Authors:
    • MC MANARA
    • K SCOTLANDI
    • M SERRA
    • D MAURICI
    • M SARTI
    • M CAMPANACCI
    • N BALDINI
  • View Affiliations

  • Published online on: May 1, 1995     https://doi.org/10.3892/ijo.6.5.1011
  • Pages: 1011-1014
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Abstract

The Colony-Stimulating Factors (CSFs) are undergoing clinical trials for their ability to stimulate the regeneration of bone marrow in patients receiving anticancer chemotherapy. However, the reported effects on the growth of tumor cell lines of different derivations, including osteosarcoma, raise the possibility that the use of these cytokines may induce proliferative effects also in residual tumor cells. In this study, we have used a panel of 12 human osteosarcoma (2 cell lines and 10 primary cultures) and 7 Ewing's sarcoma cell lines (5 cell lines and 2 primary cultures) to evaluate the presence of the G-CSF receptor by RT-PCR and the effects of recombinant Human (rHu) G-CSF on their in vitro growth ability. RT-PCR did not reveal the presence of G-CSF receptor band in any of the osteosarcoma or Ewing's cell lines examined. Moreover, after exposure to rHuG-CSF, no significant stimulatory or inhibitory effects were observed in any of the cell lines. Therefore, G-CSF may be safely used to stimulate marrow regeneration after high-dose chemotherapy both in osteosarcoma and in Ewing's sarcoma.

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May 1995
Volume 6 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
MANARA M, SCOTLANDI K, SERRA M, MAURICI D, SARTI M, CAMPANACCI M and BALDINI N: ABSENCE OF STIMULATORY EFFECT OF G-CSF ON THE GROWTH OF HUMAN SARCOMA-CELLS. Int J Oncol 6: 1011-1014, 1995
APA
MANARA, M., SCOTLANDI, K., SERRA, M., MAURICI, D., SARTI, M., CAMPANACCI, M., & BALDINI, N. (1995). ABSENCE OF STIMULATORY EFFECT OF G-CSF ON THE GROWTH OF HUMAN SARCOMA-CELLS. International Journal of Oncology, 6, 1011-1014. https://doi.org/10.3892/ijo.6.5.1011
MLA
MANARA, M., SCOTLANDI, K., SERRA, M., MAURICI, D., SARTI, M., CAMPANACCI, M., BALDINI, N."ABSENCE OF STIMULATORY EFFECT OF G-CSF ON THE GROWTH OF HUMAN SARCOMA-CELLS". International Journal of Oncology 6.5 (1995): 1011-1014.
Chicago
MANARA, M., SCOTLANDI, K., SERRA, M., MAURICI, D., SARTI, M., CAMPANACCI, M., BALDINI, N."ABSENCE OF STIMULATORY EFFECT OF G-CSF ON THE GROWTH OF HUMAN SARCOMA-CELLS". International Journal of Oncology 6, no. 5 (1995): 1011-1014. https://doi.org/10.3892/ijo.6.5.1011