EXPRESSION OF ACTIVIN AND ACTIVIN RECEPTORS IN HUMAN PROSTATIC-CARCINOMA CELL-LINE DU145

  • Authors:
    • BA FURST
    • Z ZHANG
    • SY YING
  • View Affiliations

  • Published online on: August 1, 1995     https://doi.org/10.3892/ijo.7.2.239
  • Pages: 239-243
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The purpose of this study was to determine whether DU145, a human prostate cancer cell line: (a) transcribes mRNAs coding for beta A- and beta B-subunits of activin, a member of transforming growth factor beta (TGF beta) superfamily, and activin receptors I, II, and IIB; and (b) produces activin proteins. The expression and localization of the mRNAs were elucidated by the reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization techniques. The production of activin was determined by immunocytochemistry. We have observed that messenger RNAs encoding activin beta A-, beta B-subunits, and activin receptors I, II, and IIB, but not that of the alpha-subunit of inhibin, were expressed, and activin proteins, but not inhibin, were produced, by DU145 cells. Furthermore, the RT-PCR products were confirmed by DNA sequencing. It is concluded that activins and their receptors are expressed in DU145 and activins may have autocrine functions in DU145 cells.

Related Articles

Journal Cover

August 1995
Volume 7 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
FURST B, ZHANG Z and YING S: EXPRESSION OF ACTIVIN AND ACTIVIN RECEPTORS IN HUMAN PROSTATIC-CARCINOMA CELL-LINE DU145. Int J Oncol 7: 239-243, 1995
APA
FURST, B., ZHANG, Z., & YING, S. (1995). EXPRESSION OF ACTIVIN AND ACTIVIN RECEPTORS IN HUMAN PROSTATIC-CARCINOMA CELL-LINE DU145. International Journal of Oncology, 7, 239-243. https://doi.org/10.3892/ijo.7.2.239
MLA
FURST, B., ZHANG, Z., YING, S."EXPRESSION OF ACTIVIN AND ACTIVIN RECEPTORS IN HUMAN PROSTATIC-CARCINOMA CELL-LINE DU145". International Journal of Oncology 7.2 (1995): 239-243.
Chicago
FURST, B., ZHANG, Z., YING, S."EXPRESSION OF ACTIVIN AND ACTIVIN RECEPTORS IN HUMAN PROSTATIC-CARCINOMA CELL-LINE DU145". International Journal of Oncology 7, no. 2 (1995): 239-243. https://doi.org/10.3892/ijo.7.2.239