COMBINATIONS OF TNP-470 OR SR-4233 WITH ANTITUMOR ALKYLATING-AGENTS IN EMT-6 SPHEROIDS AND MONOLAYERS

  • Authors:
    • SA HOLDEN
    • BA TEICHER
  • View Affiliations

  • Published online on: October 1, 1995     https://doi.org/10.3892/ijo.7.4.777
  • Pages: 777-781
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Abstract

EMT-6/Parent and EMT-6/CDDP and EMT-6/CTX in vivo alkylating agent resistant cells were grown as spheroids or as monolayers and their response to cis-diamminedichloroplatinum(II) or 4-hydroperoxycyclophosphamide exposure for 1 h alone or in combination with TNP-470 or SR-4233 was determined. When grown as spheroids, each of the three cell lines were less responsive to cis-diamminedichloroplatinum(II) and 4-hydroperoxycyclophosphamide exposure than when the cells were grown and drug-treated in monolayer. The hypoxic cell selective cytotoxic agent SR-4233 was additive in cytotoxicity with the antitumor alkylating agents in both the monolayer and spheroid cultures as determined by isobologram analysis. The antiangiogenic agent TNP-470 was synergistic in cytotoxicity in combination with cis-diammedichloroplatinum in each of the three cell lines when the cells were grown in monolayer and was additive in cytotoxicity with cis-diamminedichloroplatinum(II) when the cells were grown as spheroids. The combination of TNP-470 and 4-hydroperoxycyclophosphamide resulted in additive cytotoxicity toward both monolayer cultures and spheroids. Thus, co-exposure with TNP-470 or SR-4233 increased the cytotoxicity of the antitumor alkylating agents in both the parental and drug resistant cells grown as monolayers or spheroids.

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October 1995
Volume 7 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
HOLDEN S and HOLDEN S: COMBINATIONS OF TNP-470 OR SR-4233 WITH ANTITUMOR ALKYLATING-AGENTS IN EMT-6 SPHEROIDS AND MONOLAYERS. Int J Oncol 7: 777-781, 1995
APA
HOLDEN, S., & HOLDEN, S. (1995). COMBINATIONS OF TNP-470 OR SR-4233 WITH ANTITUMOR ALKYLATING-AGENTS IN EMT-6 SPHEROIDS AND MONOLAYERS. International Journal of Oncology, 7, 777-781. https://doi.org/10.3892/ijo.7.4.777
MLA
HOLDEN, S., TEICHER, B."COMBINATIONS OF TNP-470 OR SR-4233 WITH ANTITUMOR ALKYLATING-AGENTS IN EMT-6 SPHEROIDS AND MONOLAYERS". International Journal of Oncology 7.4 (1995): 777-781.
Chicago
HOLDEN, S., TEICHER, B."COMBINATIONS OF TNP-470 OR SR-4233 WITH ANTITUMOR ALKYLATING-AGENTS IN EMT-6 SPHEROIDS AND MONOLAYERS". International Journal of Oncology 7, no. 4 (1995): 777-781. https://doi.org/10.3892/ijo.7.4.777