ACTIVIN AND ACTIVIN RECEPTORS IN A RAT OSTEOGENIC-SARCOMA CELL-LINE, UMR-106

  • Authors:
    • SY YING
    • Z ZHANG
    • G HUANG
    • Y BATRES
  • View Affiliations

  • Published online on: November 1, 1995     https://doi.org/10.3892/ijo.7.5.1061
  • Pages: 1061-1065
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Abstract

We have localized immunocytochemically activin-A-like reactivity in UMR-106 osteogenic cells with a characterized, specific antiserum against synthetic fragment of subunits of activin-A, a member of transforming growth factor B (TGFB) superfamily. No staining was seen when the activin BA-subunit antisera were replaced by immunoneutralized sera, antiserum dilution buffer, or normal rabbit serum. Using in situ hybridization and the reverse transcription polymerase chain reaction (RT-PCR), we have observed the expression of the BA-subunit of activin and activin receptors II & IIB, DNA sequencing data show that the RT-PCR product corresponds to the predicted fragments of activin-A and activin receptors, respectively. In addition, exogenously administered activin inhibited cell growth in cultured UMR-106 cells. Our findings have shown that (a) activin-A was localized immunohistochemically to UMR-106 cells, (b) expression of activin-A and its receptors are detected by in situ hybridization and RT-PCR in UMR-106 cells, and (c) activin may have an inhibitory autocrine function in the proliferation of these cells.

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November 1995
Volume 7 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
YING S, ZHANG Z, HUANG G and BATRES Y: ACTIVIN AND ACTIVIN RECEPTORS IN A RAT OSTEOGENIC-SARCOMA CELL-LINE, UMR-106. Int J Oncol 7: 1061-1065, 1995
APA
YING, S., ZHANG, Z., HUANG, G., & BATRES, Y. (1995). ACTIVIN AND ACTIVIN RECEPTORS IN A RAT OSTEOGENIC-SARCOMA CELL-LINE, UMR-106. International Journal of Oncology, 7, 1061-1065. https://doi.org/10.3892/ijo.7.5.1061
MLA
YING, S., ZHANG, Z., HUANG, G., BATRES, Y."ACTIVIN AND ACTIVIN RECEPTORS IN A RAT OSTEOGENIC-SARCOMA CELL-LINE, UMR-106". International Journal of Oncology 7.5 (1995): 1061-1065.
Chicago
YING, S., ZHANG, Z., HUANG, G., BATRES, Y."ACTIVIN AND ACTIVIN RECEPTORS IN A RAT OSTEOGENIC-SARCOMA CELL-LINE, UMR-106". International Journal of Oncology 7, no. 5 (1995): 1061-1065. https://doi.org/10.3892/ijo.7.5.1061