Efficient adenovirus-mediated gene transfer into human cancer cell lines derived from digestive tract

  • Authors:
    • H Sekiguchi
    • K Isobe
    • S Akiyama
    • H Yi
    • H Takeshita
    • T Watanabe
    • I Saito
    • Y Kasai
    • K Ito
    • Y Kanegae
    • I Nakashima
    • H Takagi
  • View Affiliations

  • Published online on: February 1, 1996     https://doi.org/10.3892/ijo.8.2.283
  • Pages: 283-287
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Abstract

The efficiency of gene transfer into human cancer cells from digestive tract was evaluated using a replication-deficient recombinant adenovirus (Ad) vector harboring a lacZ gene of E. coli as a reporter gene (AxCALacZ). Average percent X-gal staining of esophageal cancer cell lines was 46%, that of gastric cancer cell lines 82% and that of colon cancer cell lines 70% at 3 days after Ad vector infection. X-gal staining in vitro continued 2 months after infection. By the direct injection of adenovirus vector to the tumors in nude mice, a certain percentage of tumor cells was stained by the X-gal. Colon26 cell line infected with AxCALacZ was implanted in BALB/c mice immunized with AxCALacZ, and tumor growth was suppressed. We presume this was due to anti-adenoviral immunity.

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February 1996
Volume 8 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Sekiguchi H, Isobe K, Akiyama S, Yi H, Takeshita H, Watanabe T, Saito I, Kasai Y, Ito K, Kanegae Y, Kanegae Y, et al: Efficient adenovirus-mediated gene transfer into human cancer cell lines derived from digestive tract. Int J Oncol 8: 283-287, 1996
APA
Sekiguchi, H., Isobe, K., Akiyama, S., Yi, H., Takeshita, H., Watanabe, T. ... Takagi, H. (1996). Efficient adenovirus-mediated gene transfer into human cancer cell lines derived from digestive tract. International Journal of Oncology, 8, 283-287. https://doi.org/10.3892/ijo.8.2.283
MLA
Sekiguchi, H., Isobe, K., Akiyama, S., Yi, H., Takeshita, H., Watanabe, T., Saito, I., Kasai, Y., Ito, K., Kanegae, Y., Nakashima, I., Takagi, H."Efficient adenovirus-mediated gene transfer into human cancer cell lines derived from digestive tract". International Journal of Oncology 8.2 (1996): 283-287.
Chicago
Sekiguchi, H., Isobe, K., Akiyama, S., Yi, H., Takeshita, H., Watanabe, T., Saito, I., Kasai, Y., Ito, K., Kanegae, Y., Nakashima, I., Takagi, H."Efficient adenovirus-mediated gene transfer into human cancer cell lines derived from digestive tract". International Journal of Oncology 8, no. 2 (1996): 283-287. https://doi.org/10.3892/ijo.8.2.283