Human lung carcinoma cells engineered to release IL2, IL7, GM-CSF and TNF alpha

  • Authors:
    • A Guarini
    • L Riera
    • G Reato
    • A Carbone
    • A Cignetti
    • A Tos
    • L Lanfrancone
    • C Melani
    • R Paul
    • G Forni
    • R Foa
  • View Affiliations

  • Published online on: April 1, 1996     https://doi.org/10.3892/ijo.8.4.765
  • Pages: 765-772
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

A human lung adenocarcinoma cell line (LC89) was transduced with the IL2, IL7, GM-CSF and TNF alpha genes by retroviral vector mediated infection. This induced the constitutive and stable release of all cytokines. No difference or modulation was found in the parental and gene transduced LC89 cells with regard to cytokine receptor expression, in vitro cell growth and proliferation, nor in cell surface expression of different adhesion molecules. Following injection into immunosuppressed nu/nu mice, IL2 gene transduced LC89 cells lost their tumorigenic potential. LC89 cells engineered to release IL7 and TNF alpha grew in nu/nu mice, but in 40% of the animals tumor regression was observed. GM-CSF gene transduced LC89 cells showed a tumorigenic capacity identical to that of the parental clone. The levels of TGF beta(1) released by IL2, IL7 and GM-CSF gene transduced LC89 cells were markedly reduced compared to those of the parental and TNF alpha gene transduced cells. The results of this study support the concept that human lung cancer cells engineered with different cytokine genes maintain their intrinsic morphologic and proliferative features, while their tumorigenic and immunosuppressive capacities can be profoundly down-modulated. Both these effects are optimally achieved following insertion of the IL2 gene, suggesting that vaccination protocols with IL2 gene transduced tumor cells may be considered for the management of human lung cancer.

Related Articles

Journal Cover

April 1996
Volume 8 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Guarini A, Riera L, Reato G, Carbone A, Cignetti A, Tos A, Lanfrancone L, Melani C, Paul R, Forni G, Forni G, et al: Human lung carcinoma cells engineered to release IL2, IL7, GM-CSF and TNF alpha. Int J Oncol 8: 765-772, 1996
APA
Guarini, A., Riera, L., Reato, G., Carbone, A., Cignetti, A., Tos, A. ... Foa, R. (1996). Human lung carcinoma cells engineered to release IL2, IL7, GM-CSF and TNF alpha. International Journal of Oncology, 8, 765-772. https://doi.org/10.3892/ijo.8.4.765
MLA
Guarini, A., Riera, L., Reato, G., Carbone, A., Cignetti, A., Tos, A., Lanfrancone, L., Melani, C., Paul, R., Forni, G., Foa, R."Human lung carcinoma cells engineered to release IL2, IL7, GM-CSF and TNF alpha". International Journal of Oncology 8.4 (1996): 765-772.
Chicago
Guarini, A., Riera, L., Reato, G., Carbone, A., Cignetti, A., Tos, A., Lanfrancone, L., Melani, C., Paul, R., Forni, G., Foa, R."Human lung carcinoma cells engineered to release IL2, IL7, GM-CSF and TNF alpha". International Journal of Oncology 8, no. 4 (1996): 765-772. https://doi.org/10.3892/ijo.8.4.765