Intrasplenic vaccination against experimental melanoma

  • Authors:
    • D Shrayer
    • C Park
    • N Kouttab
    • H Bogaars
    • R McInnis
    • S Paul
    • A Maizel
    • V Hearing
    • H Wanebo
  • View Affiliations

  • Published online on: July 1, 1996     https://doi.org/10.3892/ijo.9.1.123
  • Pages: 123-129
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Abstract

The immunodominant component of a formalinized extracellular antigen (fECA) vaccine prepared from B16 F10 melanoma cells is the melanoma-associated antigen B700. We now demonstrate that a single prophylactic intrasplenic inoculation of B700 antigen (1-10 mu g) stimulates the production of antibodies which have antiproliferative effects on B16 F10 melanoma cells in vitro. In addition, potential cytotoxic effects of splenocytes from B700 antigen inoculated mice were evaluated for two cellular immune effector functions, natural killer (NK) cell activity and lymphokine activated killer (LAK) cell activity; both activities were increased following B700 antigen inoculation. Intrasplenic injection of B700 antigen elicited an increase in the expression of the CD25 surface antigen (IL-2 R alpha) by T lymphocytes and up-regulated the expression of IL-2 R alpha mRNA. Thus both humoral and cellular cytotoxic immune responses might play roles in the decreased growth of primary tumors in B700 antigen inoculated mice and in the higher survival rate in this group of animals.

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July 1996
Volume 9 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Shrayer D, Park C, Kouttab N, Bogaars H, McInnis R, Paul S, Maizel A, Hearing V and Wanebo H: Intrasplenic vaccination against experimental melanoma. Int J Oncol 9: 123-129, 1996
APA
Shrayer, D., Park, C., Kouttab, N., Bogaars, H., McInnis, R., Paul, S. ... Wanebo, H. (1996). Intrasplenic vaccination against experimental melanoma. International Journal of Oncology, 9, 123-129. https://doi.org/10.3892/ijo.9.1.123
MLA
Shrayer, D., Park, C., Kouttab, N., Bogaars, H., McInnis, R., Paul, S., Maizel, A., Hearing, V., Wanebo, H."Intrasplenic vaccination against experimental melanoma". International Journal of Oncology 9.1 (1996): 123-129.
Chicago
Shrayer, D., Park, C., Kouttab, N., Bogaars, H., McInnis, R., Paul, S., Maizel, A., Hearing, V., Wanebo, H."Intrasplenic vaccination against experimental melanoma". International Journal of Oncology 9, no. 1 (1996): 123-129. https://doi.org/10.3892/ijo.9.1.123