Open Access

Successful therapeutic rechallenge after a severe episode of high dose methotrexate‑induced choreoathetosis: A case report

  • Authors:
    • Lip Leong Chong
    • Evelyn Yi Ting Wong
    • Sheryl Lyn Lucero Santos‑Banta
    • Chee Leong Cheng
    • Leonard Tan
    • Eileen Yi Ling Poon
    • Nagavalli Somasundaram
    • Mohamad Farid
    • Tiffany Tang
    • Miriam Tao
    • James Boon Kheng Khoo
    • Vivianne Shih
    • Daryl Ming Zhe Cheah
    • Choon Kiat Ong
    • Soon Thye Lim
    • Jason Yongsheng Chan
  • View Affiliations

  • Published online on: July 16, 2019     https://doi.org/10.3892/mco.2019.1898
  • Pages: 354-358
  • Copyright : © Chong et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Methotrexate (MTX) is an essential chemotherapy drug used in the treatment of malignancies, but it is known to cause complications to the central nervous system. We report a case of severe MTX neurotoxicity in an adult presenting with choreoathetosis despite a normal clearance of MTX. High dose‑MTX has been successfully rechallenged without any neurological sequelae. We reviewed the relevant literature of similar manifestations and summarized their clinical data, magnetic resonance imaging features and treatment given. None of them has recurrence of neurotoxicity. We concluded that it is safe to persist with MTX even after a previous episode of toxic leukoencephalopathy.

Introduction

Methotrexate is a cell-cycle specific agent which disrupts the metabolism of folic acid and DNA synthesis by inhibiting the enzyme dihydrofolate reductase. It is a commonly used antimetabolite in the treatment of adult and pediatric cancers, including lymphomas, acute lymphoblastic leukemias and osteosarcomas (1). The incidence of acute MTX neurotoxicity is reportedly 3–10% and it depends on the dose, frequency and route of administration of MTX as well as the prophylactic use of leucovorin (2). Acute encephalopathy usually develops within 5 to 14 days after HD MTX, commonly presenting as nausea, headache, seizures, altered mental status or stroke-like symptoms. Choreoathetosis is an unusual and severe presentation of MTX neurotoxicity, and to our knowledge, has not been reported in adults (3,4).

Case report

A 46 year old woman who presented with confusion and lethargy of 3 weeks duration was diagnosed with an isolated relapse of lymphoma involving the central nervous system (CNS). She had a history of diffuse large B-cell lymphoma (DLBCL) of the breast 12 years ago and was treated then with six cycles of R-CHOP chemotherapy (which included rituximab, cyclophosphamide, doxorubicin, vincristine and prednisolone) and localized radiotherapy to the breast. She did not receive CNS prophylaxis at initial diagnosis. She remained in clinical remission for ten years until she relapsed in the lumbosacral plexus. She received salvage chemotherapy with 4 cycles of R-ESHAP (rituximab, etoposide, methylprednisolone, cytarabine and cisplatin) and went into complete remission.

At the current presentation, gadolinium-enhanced magnetic resonance imaging (MRI) of the brain revealed subcortical white matter hyperintensities on fluid-attenuated inversion recovery (FLAIR) sequence with associated vascular and leptomeningeal enhancement consistent with lymphoma recurrence (Fig. 1). Diagnostic lumbar puncture confirmed the presence of CD20-positive lymphomatous large B cells, typical of leptomeningeal disease (Fig. 2). These large cells also intensely co-expressed CD79a with a Ki-67 proliferation fraction in the region of 60–70% on immunohistochemistry. Flow cytometry analysis was negative for clonal B lymphocytic cells however, this might have been a false negative result due to either limited CSF sample or rapid degeneration of viable lymphocytes. There was no scintigraphic evidence of lymphoma recurrence elsewhere on whole body 18F-FDG PET/CT scan.

She was commenced on dexamethasone, intravenous rituximab and HD MTX infusion at 3.5 g/m2 given over 3 h based on Shah's protocol for treatment of newly-diagnosed primary CNS lymphoma (5). IV folinic acid 30 mg 6-hourly was given 24 h after the start of HD MTX infusion until MTX levels <0.05 µmol/l had been achieved (Fig. 3). There was no incidence of toxicity observed on serial monitoring of plasma MTX concentrations. The decrease in serum methotrexate level with time after the start of HD MTX, compared to the baseline methotrexate level was analyzed with Friedman test. All statistical analyses were performed using SPSS17.0 software. A P-value <0.05 (two-tailed) was considered to be of statistical significance.

Four days after HD MTX infusion, she developed sudden jerking movements of the upper limbs with drooling of saliva and extensor posturing of the body. She suffered another complex partial seizure with secondary generalization a few hours later, which aborted spontaneously. She did not have any fever, hypoglycaemia or signs of meningism. Neurological examination was otherwise normal. An urgent CT scan of the brain did not identify any new intracranial lesions nor was there any epileptiform activity on an electroencephalogram (EEG) performed the following day. She was subsequently treated with levetiracetam and there had been no recurrence of seizure since.

Two days later, she was observed to have prolonged involuntary and irregular movements involving bilateral upper extremities with intermittent writhing of the neck and trunk, which would resolve when she slept but recurred when she was awake. A repeat MRI of the brain showed extensive T2 and FLAIR hyperintensities with bilateral and symmetrical involvement of the basal ganglia and periventricular white matter, some of which demonstrated restricted diffusion without any associated solid enhancement. Notably, the previously seen vascular and leptomeningeal enhancement have improved. Further investigations to exclude other causes of choreoathetosis were performed: serum ceruloplasmin, thyroid function, creatine kinase and ferritin were normal, anti-streptolysin O titre (ASOT) and autoimmune workup were negative; as well as a peripheral blood film which did not yield any acanthocytes. A neurologist was consulted for her movement disorder and she was started on tetrabenazine 12.5 mg bd with complete resolution of her symptoms thereafter.

She was successfully rechallenged with HD MTX at a lower dose of 2.5 g/m2 at week 3 without any recurrence of neurotoxicity, completing a total of 5 biweekly doses. In addition, she received 2 doses of intrathecal MTX 12 mg at weeks 7 and 9 without any complications. MRI brain was repeated after two months which had shown near complete resolution of white matter changes in the basal ganglia while her lymphoma remained in clinical and radiological remission.

Discussion

We described a case of acute, reversible methotrexate neurotoxicity in an adult patient with lymphoma who was successfully rechallenged with HD MTX. The differential diagnoses of chorea are broad and can be caused by any structural, metabolic, infectious, autoimmune or malignant involvement of the basal ganglia (6). The bilateral and symmetrical involvements of the basal ganglia in addition to the periventricular white matter were severe and directly accounted for the choreoathetosis observed (7). The putamen and globus pallidus were high in metabolic activity due to an abundance of vascular supply, mitochondria and neurotransmitters compared with other regions of the brain (8). Therefore, this was more suggestive of a toxic-metabolic cause of acute leukoencephalopathy rather than a unilateral neoplastic process.

The diagnosis of MTX-induced encephalopathy in our patient was made based on transient symptoms with full recovery, typical radiographic features and exclusion of other causes after an extensive panel of investigations. We reviewed a series of case reports whereby chorea was one of the main presenting symptoms and summarized them in the tables below (Tables I and II). All the previously reported patients were diagnosed with pediatric ALL on intrathecal MTX with or without HD MTX prior to their neurotoxic symptoms (911). MRI brain typically showed focal areas of restricted diffusion, particularly in the basal ganglia and centrum semiovale, which disappeared on follow up imaging. These radiographic findings of transient restricted diffusion in the absence of vascular or perfusion changes were consistent with reversible cytotoxic edema of the white matter from acute MTX leukoencephalopathy (12). All of them, except patient number 2 whom had completed treatment, had received further doses of intrathecal or HD MTX subsequently without any recurrence of neurotoxicity.

Table I.

Clinical features of 5 patients with acute methotrexate neurotoxicity and successful outcomes after rechallenge with MTX.

Table I.

Clinical features of 5 patients with acute methotrexate neurotoxicity and successful outcomes after rechallenge with MTX.

No.Age (years)SexDiagnosisRoute of MTX prior to eventTime to event (days)Neurological symptomsDuration (days)
112MaleALLHD and IT MTX9Hemiparesis, bilateral weakness, dysphasia, confusion, emotionality and chorea1
2  7FemaleALLHD and IT MTX8Hemiparesis, confusion, emotionality and chorea6
3  6FemaleALLIT MTX only4Hypotonia in all limbs and chorea7
417MaleALLIT MTX onlyUnknownNausea, headache, confusion, right hemiparesis and chorea<10
5a46FemaleDLBCLHD MTX only4Seizure, confusion and chorea5

a Described in the present case report. ALL, acute lymphoblastic leukemia; DLBCL, diffuse large B cell lymphoma; MTX, methotrexate; HD MTX, high-dose methotrexate; IT MTX, intrathecal methotrexate.

Table II.

MRI imaging features and successful outcomes of 5 patients after rechallenge with MTX.

Table II.

MRI imaging features and successful outcomes of 5 patients after rechallenge with MTX.

Author, yearNo.MRI imaging features at initial presentationMRI imaging features on follow-up (duration)TreatmentSubsequent doses of MTX givenRecurrence of neurotoxicity(Refs.)
Inaba et al, 20081Restricted diffusion in the bilateral centrum semiovale, corona radiata and internal capsulesNormal diffusion with persistent T2 and FLAIR signal increase (7 days)AminophyllineHD and IT MTXNo9
Inaba et al, 20082Restricted diffusion in the bilateral basal ganglia, putamina and caudate headsIncreased diffusion with residual small left periatrial white matter signal on T2 and FLAIR (5 months)AminophyllineNoneaNo9
Lorazepam, clonazepam
Diphenhydramine
Necioğlu et al, 20093No signal abnormalitiesNAHaloperidolIT MTXNo10
Bota and Dafer, 20094Restricted diffusion in the bilateral centrum semiovaleNormal diffusion with persistent T2 and FLAIR signal increase (2 months)Folinic acid DexamethasoneIT MTXNo11
NA5bRestricted diffusion in the bilateral basal ganglia and periventricular white matterNormal diffusion with persistent T2 and FLAIR signal increase (2 months)Folinic acid TetrabenazineHD and IT MTXNoNA

a Completed planned HD MTX and did not require further chemotherapy.

b Described in the present case report. MRI, magnetic resonance imaging; NA, not applicable; MTX, methotrexate; HD MTX, high-dose methotrexate; IT MTX, intrathecal methotrexate.

There are differences in the treatments used in managing acute MTX neurotoxicity, probably because the exact pathophysiology is still not well understood. The plasma MTX level does not correlate with the incidence of acute neurotoxicity and this relationship is not well-established compared to other adverse effects of MTX such as nephrotoxicity (13). Observations from our patient and case reports from Hong Kong have illustrated that there are no instances of delayed elimination of MTX preceding the neurological symptoms (14). It is widely believed that MTX can cause direct toxic damage to the CNS, potentially by the accumulation of adenosine after MTX inhibition on purine synthesis (15). Methylxanthines have therefore been used in some cases based on their ability to displace adenosine from the central receptors (16). We did not employ methylxanthines in the treatment of our patient but we had continued on folinic acid for another week. Importantly, the patient went on to receive both HD MTX and intrathecal MTX successfully.

Prompt recognition of an acute toxic leukoencephalopathy is important in the management of patients presenting with neurological symptoms and known recent exposure to MTX. This would prevent unnecessary investigations and a delay in the diagnosis of acute MTX neurotoxicity. Diffusion-weighted MRI is a useful imaging modality to detect early changes of cytotoxic edema, which are often transient and reversible (17). It is safe to persist with MTX and that a prior episode of MTX-induced neurotoxicity does not preclude its future use as MTX remains an effective drug in the management of CNS lymphoma.

Acknowledgements

Not applicable.

Funding

No funding was received.

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Authors' contributions

LC and JC contributed to the conception of the study and wrote the manuscript. LC, EW, SS, EP, NS, MF, TT, VS, MT, JC and SL provided clinical assistance and contributed to the acquisition of clinical data. CC, LT, DC and CO reviewed the slides and contributed to pathological data acquisition and interpretation. JK analyzed the images, and interpreted and acquired the radiological data. All the authors read and approved the final version of this manuscript.

Ethics approval and consent to participate

Not applicable.

Patient consent for publication

The patient provided written informed consent for the publication of any associated data and accompanying images.

Competing interests

The authors declare that they have no competing interests.

References

1 

Howard SC, McCormick J, Pui CH, Buddington RK and Harvey RD: Preventing and managing toxicities of high-dose methotrexate. Oncologist. 21:1471–1482. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Rollins N, Winick N, Bash R and Booth T: Acute methotrexate neurotoxicity: Findings on diffusion-weighted imaging and correlation with clinical outcome. AJNR AM J Neuroradiol. 25:1688–1695. 2004.PubMed/NCBI

3 

Vagace JM, de la Maya MD, Caceres-Marzal C, Gonzalez de Murillo S and Gervasini G: Central nervous system chemotoxicity during treatment of pediatric acute lymphoblastic leukemia/lymphoma. Crit Rev Oncol Hematol. 84:274–286. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Bhojwani D, Sabin ND, Pei D, Yang JJ, Khan RB, Panetta JC, Krull KR, Inaba H, Rubnitz JE, Metzger ML, et al: Methotrexate-induced neurotoxicity and leukoencephalopathy in childhood acute lymphoblastic leukemia. J Clin Oncol. 32:949–959. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Shah GD, Yahalom J, Correa DD, Lai RK, Raizer JJ, Schiff D, LaRocca R, Grant B, DeAngelis LM and Abrey LE: Combined immunochemotherapy with reduced whole-brain radiotherapy for newly diagnosed primary CNS lymphoma. J Clin Oncology. 25:4730–4735. 2007. View Article : Google Scholar

6 

Walker RH: Differential diagnosis of chorea. Curr Neurol Neurosci Rep. 11:385–395. 2011. View Article : Google Scholar : PubMed/NCBI

7 

McKinney AM, Kieffer SA, Paylor RT, SantaCruz KS, Kendi A and Lucato L: Acute toxic leukoencephalopathy: Potential for reversibility clinically and on MRI with diffusion-weighted and FLAIR imaging. AJR AM J Roentgenol. 193:192–206. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Hegde AN, Mohan S, Lath N and Lim CC: Differential diagnosis for bilateral abnormalities of the basal ganglia and thalamus. Radiographics. 31:5–30. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Inaba H, Khan RB, Laningham FH, Crews KR, Pui CH and Daw NC: Clinical and radiological characteristics of methotrexate-induced acute encephalopathy in pediatric patients with cancer. Ann Oncol. 19:178–184. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Necioğlu Orken D, Yldrmak Y, Kenangil G, Kandraloğlu N, Forta H and Celik M: Intrathecal methotrexate-induced acute chorea. J Pediatr Hematol Oncol. 31:57–58. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Bota DA and Dafer RM: Acute methotrexate neurotoxicity with choreiform movements and focal neurological deficits: A case report. South Med J. 102:1071–1074. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Valle DA, Kakehasi FM, Melo RM, Siqueira CM, Soares TF and Rodrigues KE: Stroke-like encephalopathy following high-dose intravenous methotrexate in an adolescent with osteosarcoma: A case report. Rev Bras Hematol Hemoter. 38:364–367. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Tsurusawa M, Gosho M, Mori T, Mitsui T, Sunami S, Kobayashi R, Fukano R, Tanaka F, Fujita N, Inada H, et al: Statistical analysis of relation between plasma methotrexate concentration and toxicity in high-dose methotrexate therapy of childhood nonHodgkin lymphoma. Pediatr Blood Cancer. 62:279–284. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Lam DST, Khong PL, Chiang AKS, Ha SY, Lau YL and Chan GCF: Reversible encephalopathy induced by systemic high dose methotrexate. HK J Paediatr. 12:287–292. 2007.

15 

Bernini JC, Fort DW, Griener JC, Kane BJ, Chappell WB and Kamen BA: Aminophylline for methotrexate-induced neurotoxicity. Lancet. 345:544–547. 1995. View Article : Google Scholar : PubMed/NCBI

16 

Fernández-Fernández FJ, Ameneiros-Lago E, Lijó-Carballeda C and Sesma P: Dextromethorphan and methylxanthines might be useful in the treatment of methotrexate-induced neurotoxicity. Pediatr Emerg Care. 33:3802017. View Article : Google Scholar : PubMed/NCBI

17 

Salkade PR and Lim TA: Methotrexate-induced acute toxic leukoencephalopathy. J Cancer Res Ther. 8:292–296. 2012. View Article : Google Scholar : PubMed/NCBI

Related Articles

Journal Cover

October-2019
Volume 11 Issue 4

Print ISSN: 2049-9450
Online ISSN:2049-9469

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Chong LL, Wong EY, Santos‑Banta SL, Cheng CL, Tan L, Poon EY, Somasundaram N, Farid M, Tang T, Tao M, Tao M, et al: Successful therapeutic rechallenge after a severe episode of high dose methotrexate‑induced choreoathetosis: A case report. Mol Clin Oncol 11: 354-358, 2019
APA
Chong, L.L., Wong, E.Y., Santos‑Banta, S.L., Cheng, C.L., Tan, L., Poon, E.Y. ... Chan, J.Y. (2019). Successful therapeutic rechallenge after a severe episode of high dose methotrexate‑induced choreoathetosis: A case report. Molecular and Clinical Oncology, 11, 354-358. https://doi.org/10.3892/mco.2019.1898
MLA
Chong, L. L., Wong, E. Y., Santos‑Banta, S. L., Cheng, C. L., Tan, L., Poon, E. Y., Somasundaram, N., Farid, M., Tang, T., Tao, M., Khoo, J. B., Shih, V., Cheah, D. M., Ong, C. K., Lim, S. T., Chan, J. Y."Successful therapeutic rechallenge after a severe episode of high dose methotrexate‑induced choreoathetosis: A case report". Molecular and Clinical Oncology 11.4 (2019): 354-358.
Chicago
Chong, L. L., Wong, E. Y., Santos‑Banta, S. L., Cheng, C. L., Tan, L., Poon, E. Y., Somasundaram, N., Farid, M., Tang, T., Tao, M., Khoo, J. B., Shih, V., Cheah, D. M., Ong, C. K., Lim, S. T., Chan, J. Y."Successful therapeutic rechallenge after a severe episode of high dose methotrexate‑induced choreoathetosis: A case report". Molecular and Clinical Oncology 11, no. 4 (2019): 354-358. https://doi.org/10.3892/mco.2019.1898