c-Jun-mediated repression and transactivation of fibronectin

  • Authors:
    • Yukio Hayashi
    • Yuji Shino
    • Kengo Saito
    • Hideki Tanzawa
    • Hiroshi Shirasawa
  • View Affiliations

  • Published online on: January 1, 2008     https://doi.org/10.3892/mmr.1.1.99
  • Pages: 99-103
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Abstract

Fibronectin (FN) is transactivated by human papillomavirus type 16 (HPV16) E6 via the induction of c-Jun-ATF-2 complexes binding to the cyclic AMP response element (CRE) in the FN promoter. The present study analyzed c-Jun regulation of FN gene expression. Northern and immunoblot analyses showed that c-Jun expression was enhanced in HPV16-E6-expressing cells. However, mouse 10T1/2 cell lines overexpressing c-Jun showed an inverse correlation between the expression levels of c-Jun and those of FN. Luciferase assays indicated that the FN promoter was strongly repressed in c-Jun-overexpressing mouse 10T1/2 cells. Deletion and mutation analyses of the FN promoter revealed that repression of the FN promoter by c-Jun depends on the CRE located at -160 relative to the start site of transcription. Supershift assays of CRE-bound complexes from HPV16-E6-expressing and c-Jun-overexpressing cells suggested that the presence of ATF-2 in the complexes binding to CRE was required for the transactivation of the FN gene. Collectively, our study suggests that the FN gene can be either transactivated or repressed by c-Jun depending on the cell context.

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January-February 2008
Volume 1 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Hayashi Y, Shino Y, Saito K, Tanzawa H and Shirasawa H: c-Jun-mediated repression and transactivation of fibronectin. Mol Med Rep 1: 99-103, 2008
APA
Hayashi, Y., Shino, Y., Saito, K., Tanzawa, H., & Shirasawa, H. (2008). c-Jun-mediated repression and transactivation of fibronectin. Molecular Medicine Reports, 1, 99-103. https://doi.org/10.3892/mmr.1.1.99
MLA
Hayashi, Y., Shino, Y., Saito, K., Tanzawa, H., Shirasawa, H."c-Jun-mediated repression and transactivation of fibronectin". Molecular Medicine Reports 1.1 (2008): 99-103.
Chicago
Hayashi, Y., Shino, Y., Saito, K., Tanzawa, H., Shirasawa, H."c-Jun-mediated repression and transactivation of fibronectin". Molecular Medicine Reports 1, no. 1 (2008): 99-103. https://doi.org/10.3892/mmr.1.1.99