Involvement of Rac in thromboxane A2‑induced human platelet activation: Regulation of sCD40 ligand release and PDGF‑AB secretion

  • Authors:
    • Yasunari Kageyama
    • Tomoaki Doi
    • Rie Matsushima‑Nishiwaki
    • Yuko Iida
    • Shigeru Akamatsu
    • Akira Kondo
    • Gen Kuroyanagi
    • Naohiro Yamamoto
    • Jun Mizutani
    • Takanobu Otsuka
    • Haruhiko Tokuda
    • Hiroki Iida
    • Osamu Kozawa
    • Shinji Ogura
  • View Affiliations

  • Published online on: April 15, 2014     https://doi.org/10.3892/mmr.2014.2143
  • Pages: 107-112
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Abstract

We have previously shown that glycoprotein Ib/IX/V activation stimulates the release of the soluble CD40 ligand (sCD40L) via the generation of thromboxane A2 from human platelets. In the present study, the role of Rac, which is a member of the Rho family, was investigated in the thromboxane A2‑stimulated release of platelet‑derived growth factor (PDGF)‑AB and sCD40L in human platelets. U46619, a thromboxane receptor agonist, stimulated the activation of Rac time‑dependently in human platelets, and NSC23766, a selective inhibitor of the Rac‑guanine nucleotide exchange factor interaction, reduced the U46619‑induced platelet aggregation. NSC23766 markedly suppressed the U46619‑induced p38 mitogen-activated protein (MAP) kinase phosphorylation. The thromboxane A2‑induced release of PDGF‑AB and sCD40L was significantly suppressed by NSC23766 in a dose‑dependent manner. In addition, NSC23766 reduced the sCD40L release stimulated by ristocetin, a glycoprotein Ib/IX/V activator. These results indicate that Rac regulates the thromboxane A2‑induced stimulation of PDGF‑AB secretion and sCD40L release via the p38 MAP kinase in human platelets.
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July-2014
Volume 10 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Kageyama Y, Doi T, Matsushima‑Nishiwaki R, Iida Y, Akamatsu S, Kondo A, Kuroyanagi G, Yamamoto N, Mizutani J, Otsuka T, Otsuka T, et al: Involvement of Rac in thromboxane A2‑induced human platelet activation: Regulation of sCD40 ligand release and PDGF‑AB secretion. Mol Med Rep 10: 107-112, 2014
APA
Kageyama, Y., Doi, T., Matsushima‑Nishiwaki, R., Iida, Y., Akamatsu, S., Kondo, A. ... Ogura, S. (2014). Involvement of Rac in thromboxane A2‑induced human platelet activation: Regulation of sCD40 ligand release and PDGF‑AB secretion. Molecular Medicine Reports, 10, 107-112. https://doi.org/10.3892/mmr.2014.2143
MLA
Kageyama, Y., Doi, T., Matsushima‑Nishiwaki, R., Iida, Y., Akamatsu, S., Kondo, A., Kuroyanagi, G., Yamamoto, N., Mizutani, J., Otsuka, T., Tokuda, H., Iida, H., Kozawa, O., Ogura, S."Involvement of Rac in thromboxane A2‑induced human platelet activation: Regulation of sCD40 ligand release and PDGF‑AB secretion". Molecular Medicine Reports 10.1 (2014): 107-112.
Chicago
Kageyama, Y., Doi, T., Matsushima‑Nishiwaki, R., Iida, Y., Akamatsu, S., Kondo, A., Kuroyanagi, G., Yamamoto, N., Mizutani, J., Otsuka, T., Tokuda, H., Iida, H., Kozawa, O., Ogura, S."Involvement of Rac in thromboxane A2‑induced human platelet activation: Regulation of sCD40 ligand release and PDGF‑AB secretion". Molecular Medicine Reports 10, no. 1 (2014): 107-112. https://doi.org/10.3892/mmr.2014.2143