Remote ischemic postconditioning protects the heart by upregulating ALDH2 expression levels through the PI3K/Akt signaling pathway

  • Authors:
    • Ying Yu
    • Xian‑Jie Jia
    • Qiao‑Feng Zong
    • Guan‑Jun Zhang
    • Hong‑Wei Ye
    • Jie Hu
    • Qin Gao
    • Su‑Dong Guan
  • View Affiliations

  • Published online on: April 15, 2014     https://doi.org/10.3892/mmr.2014.2156
  • Pages: 536-542
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Remote ischemic postconditioning (RIPostC) has been demonstrated to protect the myocardium against ischemia/reperfusion (I/R) injury; however, the mediator and underlying mechanisms remain to be elucidated. It has been confirmed that aldehyde dehydrogenase 2 (ALDH2) is involved in the remote ischemic preconditioning pathway, but whether it is involved in RIPostC remains unknown. The aim of the present study was to determine whether increased ALDH2 expression levels were involved in the cardioprotective effect evoked by RIPostC via the phosphatidylinositol‑3‑kinase (PI3K)/Akt signaling pathway. Male Sprague Dawley rats (n=48) were randomly allocated into the following four groups: Sham group, I/R group, RIPostC group, and RIPostC plus wortmannin group (RIPostC+Wort). With the exception of the Sham group, the anesthetized rats underwent 45 min of coronary artery occlusion followed by 180 min of reperfusion to mimic an I/R injury model. Hemodynamic parameters, including the mean arterial pressure and heart rate, were recorded, the infarct size was determined and the plasma lactate dehydrogenase (LDH) content and creatine kinase (CK) activity levels were measured. The expression levels of Bcl‑2 and Bax at the mRNA level and ALDH2, Akt, phospho‑Akt (p‑Akt), caspase‑3 and cleaved caspase‑3 at the protein level in the left anterior myocardium were assessed. In the RIPostC group, the infarct size was reduced versus that of the I/R group. The plasma LDH content and CK activity levels were also reduced. The expression levels of ALDH2 protein were elevated, accompanied with increases in the levels of Bcl‑2/Bax and p‑Akt/Akt and a reduction in the levels of cleaved caspase‑3. When the PI3K inhibitor wortmannin was administered at reperfusion, the p‑Akt/Akt ratio was markedly reduced and associated with a reduction in the ALDH2 and Bcl‑2/Bax levels, and the cleaved caspase‑3 expression levels were elevated. In conclusion, ALDH2 may be an important mediator in the cardioprotection of RIPostC through the PI3K/Akt‑dependent signaling pathway.
View Figures
View References

Related Articles

Journal Cover

July-2014
Volume 10 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yu Y, Jia XJ, Zong QF, Zhang GJ, Ye HW, Hu J, Gao Q and Guan SD: Remote ischemic postconditioning protects the heart by upregulating ALDH2 expression levels through the PI3K/Akt signaling pathway. Mol Med Rep 10: 536-542, 2014
APA
Yu, Y., Jia, X., Zong, Q., Zhang, G., Ye, H., Hu, J. ... Guan, S. (2014). Remote ischemic postconditioning protects the heart by upregulating ALDH2 expression levels through the PI3K/Akt signaling pathway. Molecular Medicine Reports, 10, 536-542. https://doi.org/10.3892/mmr.2014.2156
MLA
Yu, Y., Jia, X., Zong, Q., Zhang, G., Ye, H., Hu, J., Gao, Q., Guan, S."Remote ischemic postconditioning protects the heart by upregulating ALDH2 expression levels through the PI3K/Akt signaling pathway". Molecular Medicine Reports 10.1 (2014): 536-542.
Chicago
Yu, Y., Jia, X., Zong, Q., Zhang, G., Ye, H., Hu, J., Gao, Q., Guan, S."Remote ischemic postconditioning protects the heart by upregulating ALDH2 expression levels through the PI3K/Akt signaling pathway". Molecular Medicine Reports 10, no. 1 (2014): 536-542. https://doi.org/10.3892/mmr.2014.2156