Inhibition of autophagy by 3-methyladenine potentiates sulforaphane-induced cell death of BE(2)-C human neuroblastoma cells

  • Authors:
    • Irena Horwacik
    • Monika Gaik
    • Małgorzata Durbas
    • Elżbieta Boratyn
    • Grzegorz Zając
    • Katarzyna Szychowska
    • Małgorzata Szczodrak
    • Henryk Kołoczek
    • Hanna Rokita
  • View Affiliations

  • Published online on: February 18, 2015     https://doi.org/10.3892/mmr.2015.3377
  • Pages: 535-542
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Abstract

Sulforaphane (SFN) is an isothiocyanate present in cruciferous vegetables, which has been shown to exert an anti‑cancer effect when tested in vitro and in vivo. The anti‑cancer effects of SFN encompass induction of cytoprotective autophagy; therefore, the present study aimed to determine whether the chemopreventive activity of SFN may be potentiated by inhibition of autophagy. The present study provided detailed insight into the susceptibility of human neuroblastoma cells to treatment with synthetic SFN, in combination with an inhibitor of autophagy, 3‑methyladenine (3‑MA). The present study confirmed the suppression of the viability of the human neuroblastoma cell line BE(2)‑C by SFN and reported the inhibition of DNA synthesis, as determined by a decrease in tritiated thymidine incorporation. Furthermore, the results verified the effectiveness of SFN in inducing apoptosis in the BE(2)‑C cell line as demonstrated by caspase activation, increased protein expression levels of B‑cell lymphoma 2‑associated X protein and loss of mitochondrial membrane potential. Combined treatment of the cells with SFN with 3‑MA proved to be effective in decreasing cell viability, through a mechanism that may proceed via the early induction of autophagy by SFN, followed by induction of apoptosis, as well as inhibition of autophagy by 3‑MA.
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July-2015
Volume 12 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Horwacik I, Gaik M, Durbas M, Boratyn E, Zając G, Szychowska K, Szczodrak M, Kołoczek H and Rokita H: Inhibition of autophagy by 3-methyladenine potentiates sulforaphane-induced cell death of BE(2)-C human neuroblastoma cells. Mol Med Rep 12: 535-542, 2015
APA
Horwacik, I., Gaik, M., Durbas, M., Boratyn, E., Zając, G., Szychowska, K. ... Rokita, H. (2015). Inhibition of autophagy by 3-methyladenine potentiates sulforaphane-induced cell death of BE(2)-C human neuroblastoma cells. Molecular Medicine Reports, 12, 535-542. https://doi.org/10.3892/mmr.2015.3377
MLA
Horwacik, I., Gaik, M., Durbas, M., Boratyn, E., Zając, G., Szychowska, K., Szczodrak, M., Kołoczek, H., Rokita, H."Inhibition of autophagy by 3-methyladenine potentiates sulforaphane-induced cell death of BE(2)-C human neuroblastoma cells". Molecular Medicine Reports 12.1 (2015): 535-542.
Chicago
Horwacik, I., Gaik, M., Durbas, M., Boratyn, E., Zając, G., Szychowska, K., Szczodrak, M., Kołoczek, H., Rokita, H."Inhibition of autophagy by 3-methyladenine potentiates sulforaphane-induced cell death of BE(2)-C human neuroblastoma cells". Molecular Medicine Reports 12, no. 1 (2015): 535-542. https://doi.org/10.3892/mmr.2015.3377