Open Access

Characterization of highly proliferative secondary tumor clusters along host blood vessels in malignant glioma

  • Authors:
    • Ting‑Chung Wang
    • Chun‑Yu Cheng
    • Wei‑Hsun Yang
    • Wen‑Cheng Chen
    • Pey‑Jium Chang
  • View Affiliations

  • Published online on: August 18, 2015     https://doi.org/10.3892/mmr.2015.4228
  • Pages: 6435-6444
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the extensive invasion of tumor cells into normal brain tissue, a life‑threatening feature of malignant gliomas. How invasive tumor cells migrate into normal brain tissue and form a secondary tumor structure remains to be elucidated. In the present study, the morphological and phenotypic changes of glioma cells during invasion in a C6 glioma model were investigated. C6 glioma cells were stereotactically injected into the right putamen region of adult Sprague‑Dawley rats. The brain tissue sections were then subjected to hematoxylin and eosin, immunohistochemical or immunofluorescent staining. High magnification views of the tissue sections revealed that C6 cells formed tumor spheroids following implantation and marked invasion was observed shortly after spheroid formation. In the later stages of invasion, certain tumor cells invaded the perivascular space and formed small tumor clusters. These small tumor clusters exhibited certain common features, including tumor cell multilayers surrounding an arteriole, which occurred up to several millimeters away from the primary tumor mass; a high proliferation rate; and similar gene expression profiles to the primary tumor. In conclusion, the present study revealed that invading tumor cells are capable of forming highly proliferative cell clusters along arterioles near the tumor margin, which may be a possible cause of the recurrence of malignant glioma.
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November-2015
Volume 12 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wang TC, Cheng CY, Yang WH, Chen WC and Chang PJ: Characterization of highly proliferative secondary tumor clusters along host blood vessels in malignant glioma. Mol Med Rep 12: 6435-6444, 2015
APA
Wang, T., Cheng, C., Yang, W., Chen, W., & Chang, P. (2015). Characterization of highly proliferative secondary tumor clusters along host blood vessels in malignant glioma. Molecular Medicine Reports, 12, 6435-6444. https://doi.org/10.3892/mmr.2015.4228
MLA
Wang, T., Cheng, C., Yang, W., Chen, W., Chang, P."Characterization of highly proliferative secondary tumor clusters along host blood vessels in malignant glioma". Molecular Medicine Reports 12.5 (2015): 6435-6444.
Chicago
Wang, T., Cheng, C., Yang, W., Chen, W., Chang, P."Characterization of highly proliferative secondary tumor clusters along host blood vessels in malignant glioma". Molecular Medicine Reports 12, no. 5 (2015): 6435-6444. https://doi.org/10.3892/mmr.2015.4228