Open Access

Melatonin attenuates hypertension-induced renal injury partially through inhibiting oxidative stress in rats

  • Authors:
    • Yu‑Feng Qiao
    • Wen‑Juan Guo
    • Lu Li
    • Shan Shao
    • Xi Qiao
    • Jin‑Jin Shao
    • Qiong Zhang
    • Rong‑Shan Li
    • Li‑Hua Wang
  • View Affiliations

  • Published online on: November 2, 2015     https://doi.org/10.3892/mmr.2015.4495
  • Pages: 21-26
  • Copyright: © Qiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the protective effects of melatonin (MLT) on hypertension-induced renal injury and identify its mechanism of action. Twenty-four healthy male Wistar rats were divided into a sham control group (n=8), which was subjected to sham operation and received vehicle treatment (physiological saline intraperitoneally at 0.1 ml/100 g), a vehicle group (n=8), which was subjected to occlusion of the left renal artery and vehicle treatment, and the MLT group (n=8), which was subjected to occlusion of the left renal artery and treated with MLT (10 mg/kg/day). Pathological features of the renal tissues were determined using hematoxylin and eosin staining and Masson staining. Urine protein, serum creatinine (Scr), superoxide dismutase (SOD) and malondialdehyde (MDA) were determined. Immunohistochemical analysis was performed to determine the expression of heme oxygenase‑1 (HO‑1), intercellular adhesion molecule‑1 (ICAM‑1), inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS). Furthermore, reverse transcription polymerase chain reaction was conducted to determine the mRNA expression of HO‑1, ICAM‑1, eNOS and iNOS. A marked decrease in blood pressure was noticed in the MLT group at week 4 compared with that of the vehicle group (P<0.01). Furthermore, MLT treatment attenuated the infiltration of inflammatory cells and oedema/atrophy of renal tubules. MLT attenuated hypertension-induced increases in urine protein excretion, serum creatinine and MDA as well as decreases in SOD activity in renal tissues. Furthermore, MLT attenuated hypertension-induced increases in iNOS and ICAM‑1 as well as decreases in eNOS and HO‑1 expression at the mRNA and protein level. In conclusion, the results of the present study indicated that MLT had protective roles in hypertension‑induced renal injury. Its mechanism of action is, at least in part, associated with the inhibition of oxidative stress.
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January-2016
Volume 13 Issue 1

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Spandidos Publications style
Qiao YF, Guo WJ, Li L, Shao S, Qiao X, Shao JJ, Zhang Q, Li RS and Wang LH: Melatonin attenuates hypertension-induced renal injury partially through inhibiting oxidative stress in rats. Mol Med Rep 13: 21-26, 2016
APA
Qiao, Y., Guo, W., Li, L., Shao, S., Qiao, X., Shao, J. ... Wang, L. (2016). Melatonin attenuates hypertension-induced renal injury partially through inhibiting oxidative stress in rats. Molecular Medicine Reports, 13, 21-26. https://doi.org/10.3892/mmr.2015.4495
MLA
Qiao, Y., Guo, W., Li, L., Shao, S., Qiao, X., Shao, J., Zhang, Q., Li, R., Wang, L."Melatonin attenuates hypertension-induced renal injury partially through inhibiting oxidative stress in rats". Molecular Medicine Reports 13.1 (2016): 21-26.
Chicago
Qiao, Y., Guo, W., Li, L., Shao, S., Qiao, X., Shao, J., Zhang, Q., Li, R., Wang, L."Melatonin attenuates hypertension-induced renal injury partially through inhibiting oxidative stress in rats". Molecular Medicine Reports 13, no. 1 (2016): 21-26. https://doi.org/10.3892/mmr.2015.4495