MicroRNA-200b suppresses cell invasion and metastasis by inhibiting the epithelial-mesenchymal transition in cervical carcinoma

  • Authors:
    • Yan‑Xiang Cheng
    • Qi‑Fan Zhang
    • Li Hong
    • Feng Pan
    • Jin‑Ling Huang
    • Bing‑Shu Li
    • Min Hu
  • View Affiliations

  • Published online on: February 19, 2016     https://doi.org/10.3892/mmr.2016.4911
  • Pages: 3155-3160
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Abstract

The expression of microRNA (miR)-200b is suppressed in numerous tumor types, leading to epithelial-mesenchymal transition, which enables solid tissue epithelial cancers to invade and metastasize. The present study assessed the role of miR-200b in cervical cancer with the aim of clarifying the underlying pathophysiological mechanisms and to identify potential strategies for its prevention and treatment of cervical cancer. Reverse‑transcription quantitative PCR revealed that miR‑200b was downregulated in invasive cervical carcinoma tissues compared with that in normal adjacent tissues. A Transwell migration assay indicated that transfection of cervical cancer cells with miR‑200b mimics significantly inhibited their migratory potential, while migration was enhanced in cells transfected with miR‑200b inhibitor. Furthermore, western blot analysis indicated a negative correlation between miR‑200b and mesenchymal marker vimentin as well as matrix metalloproteinase‑9, which has a key role in tumor invasion and metastasis. In addition, a positive correlation between miR‑200b and the epithelial marker E‑cadherin was revealed by western blot and immunofluorescence. The results of the present study suggested that miR‑200b suppressed the migratory potential of cervical carcinoma cells and therefore their ability to metastasize by inhibiting the epithelial-mesenchymal transition, which may be utilized for the treatment of cervical cancer.
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April-2016
Volume 13 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Cheng YX, Zhang QF, Hong L, Pan F, Huang JL, Li BS and Hu M: MicroRNA-200b suppresses cell invasion and metastasis by inhibiting the epithelial-mesenchymal transition in cervical carcinoma. Mol Med Rep 13: 3155-3160, 2016
APA
Cheng, Y., Zhang, Q., Hong, L., Pan, F., Huang, J., Li, B., & Hu, M. (2016). MicroRNA-200b suppresses cell invasion and metastasis by inhibiting the epithelial-mesenchymal transition in cervical carcinoma. Molecular Medicine Reports, 13, 3155-3160. https://doi.org/10.3892/mmr.2016.4911
MLA
Cheng, Y., Zhang, Q., Hong, L., Pan, F., Huang, J., Li, B., Hu, M."MicroRNA-200b suppresses cell invasion and metastasis by inhibiting the epithelial-mesenchymal transition in cervical carcinoma". Molecular Medicine Reports 13.4 (2016): 3155-3160.
Chicago
Cheng, Y., Zhang, Q., Hong, L., Pan, F., Huang, J., Li, B., Hu, M."MicroRNA-200b suppresses cell invasion and metastasis by inhibiting the epithelial-mesenchymal transition in cervical carcinoma". Molecular Medicine Reports 13, no. 4 (2016): 3155-3160. https://doi.org/10.3892/mmr.2016.4911