Open Access

Mangiferin inhibits macrophage classical activation via downregulating interferon regulatory factor 5 expression

  • Authors:
    • Zhiquan Wei
    • Li Yan
    • Yixin Chen
    • Chuanhong Bao
    • Jing Deng
    • Jiagang Deng
  • View Affiliations

  • Published online on: May 27, 2016     https://doi.org/10.3892/mmr.2016.5352
  • Pages: 1091-1098
  • Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Mangiferin is a natural polyphenol and the predominant effective component of Mangifera indica Linn. leaves. For hundreds of years, Mangifera indica Linn. leaf has been used as an ingredient in numerous traditional Chinese medicine preparations for the treatment of bronchitis. However, the pharmacological mechanism of mangiferin in the treatment of bronchitis remains to be elucidated. Macrophage classical activation is important role in the process of bronchial airway inflammation, and interferon regulatory factor 5 (IRF5) has been identified as a key regulatory factor for macrophage classical activation. The present study used the THP‑1 human monocyte cell line to investigate whether mangiferin inhibits macrophage classical activation via suppressing IRF5 expression in vitro. THP‑1 cells were differentiated to macrophages by phorbol 12‑myristate 13‑acetate. Macrophages were polarized to M1 macrophages following stimulation with lipopolysaccharide (LPS)/interferon‑γ (IFN‑γ). Flow cytometric analysis was conducted to detect the M1 macrophages. Reverse transcription‑quantitative polymerase chain reaction was used to investigate cellular IRF5 gene expression. Levels of proinflammatory cytokines and IRF5 were assessed following cell culture and cellular homogenization using enzyme‑linked immunosorbent assay. IRF5 protein and nuclei co‑localization was performed in macrophages with laser scanning confocal microscope immunofluorescence analysis. The results of the present study demonstrated that mangiferin significantly inhibits LPS/IFN‑γ stimulation‑induced classical activation of macrophages in vitro and markedly decreases proinflammatory cytokine release. In addition, cellular IRF5 expression was markedly downregulated. These results suggest that the inhibitory effect of mangiferin on classical activation of macrophages may be exerted via downregulation of cellular IRF5 expression levels.
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August-2016
Volume 14 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wei Z, Yan L, Chen Y, Bao C, Deng J and Deng J: Mangiferin inhibits macrophage classical activation via downregulating interferon regulatory factor 5 expression. Mol Med Rep 14: 1091-1098, 2016
APA
Wei, Z., Yan, L., Chen, Y., Bao, C., Deng, J., & Deng, J. (2016). Mangiferin inhibits macrophage classical activation via downregulating interferon regulatory factor 5 expression. Molecular Medicine Reports, 14, 1091-1098. https://doi.org/10.3892/mmr.2016.5352
MLA
Wei, Z., Yan, L., Chen, Y., Bao, C., Deng, J., Deng, J."Mangiferin inhibits macrophage classical activation via downregulating interferon regulatory factor 5 expression". Molecular Medicine Reports 14.2 (2016): 1091-1098.
Chicago
Wei, Z., Yan, L., Chen, Y., Bao, C., Deng, J., Deng, J."Mangiferin inhibits macrophage classical activation via downregulating interferon regulatory factor 5 expression". Molecular Medicine Reports 14, no. 2 (2016): 1091-1098. https://doi.org/10.3892/mmr.2016.5352