Open Access

Endothelial lipase is upregulated by interleukin-6 partly via the p38 MAPK and p65 NF-κB signaling pathways

  • Authors:
    • Xin Yue
    • Minghui Wu
    • Hong Jiang
    • Jing Hao
    • Qinghao Zhao
    • Qing Zhu
    • Gaowa Saren
    • Yun Zhang
    • Xiaoli Zhang
  • View Affiliations

  • Published online on: June 30, 2016     https://doi.org/10.3892/mmr.2016.5457
  • Pages: 1979-1985
  • Copyright: © Yue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

To investigate the effects of inflammatory factor interleukin (IL)‑6 on the expression of endothelial lipase (EL) and its potential signaling pathways in atherosclerosis, a primary culture of human umbilical vein endothelial cells (HUVECs) was established and treated as follows: i) Control group without any treatment; ii) recombinant human (rh)IL‑6 treatment (10 ng/ml) for 0, 4, 8, 12 and 24 h; iii) p38 mitogen‑activated protein kinases (MAPKs) inhibitor (SB203580, 10 µmol/l) pretreatment for 1 h prior to rhIL‑6 (10 ng/ml) treatment; iv) nuclear factor (NF)‑κB activation inhibitor (pyrrolidine dithiocarbamate, 10 mmol/l) pretreatment for 1 h prior to rhIL‑6 (10 ng/ml) treatment. EL levels were detected by immunocytochemical staining and western blot analysis. Proliferation of HUVECs was detected by immunostaining of proliferating cell nuclear antigen (PCNA) and an MTT assay. p38 MAPK and NF‑κB p65 levels were detected by western blotting. The results showed that rhIL‑6 treatment increased EL expression and proliferation of HUVECs. NF‑κB p65 and MAPK p38 protein levels also increased in a time‑dependent manner in HUVECs after rhIL‑6 treatment. NF‑κB inhibitor and MAPK p38 inhibitor prevented the effects of rhIL‑6 on EL expression. In conclusion, inflammatory factor IL‑6 may participate in the pathogenesis of atherosclerosis by increasing EL expression and the proliferation of endothelial cells via the p38 MAPK and NF-κB signaling pathways.
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September-2016
Volume 14 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Yue X, Wu M, Jiang H, Hao J, Zhao Q, Zhu Q, Saren G, Zhang Y and Zhang X: Endothelial lipase is upregulated by interleukin-6 partly via the p38 MAPK and p65 NF-κB signaling pathways. Mol Med Rep 14: 1979-1985, 2016
APA
Yue, X., Wu, M., Jiang, H., Hao, J., Zhao, Q., Zhu, Q. ... Zhang, X. (2016). Endothelial lipase is upregulated by interleukin-6 partly via the p38 MAPK and p65 NF-κB signaling pathways. Molecular Medicine Reports, 14, 1979-1985. https://doi.org/10.3892/mmr.2016.5457
MLA
Yue, X., Wu, M., Jiang, H., Hao, J., Zhao, Q., Zhu, Q., Saren, G., Zhang, Y., Zhang, X."Endothelial lipase is upregulated by interleukin-6 partly via the p38 MAPK and p65 NF-κB signaling pathways". Molecular Medicine Reports 14.3 (2016): 1979-1985.
Chicago
Yue, X., Wu, M., Jiang, H., Hao, J., Zhao, Q., Zhu, Q., Saren, G., Zhang, Y., Zhang, X."Endothelial lipase is upregulated by interleukin-6 partly via the p38 MAPK and p65 NF-κB signaling pathways". Molecular Medicine Reports 14, no. 3 (2016): 1979-1985. https://doi.org/10.3892/mmr.2016.5457