Open Access

Reprogramming A375 cells to induced‑resembled neuronal cells by structured overexpression of specific transcription genes

  • Authors:
    • Hengzhu Zhang
    • Min Wei
    • Yangyang Jiang
    • Xiaodong Wang
    • Lei She
    • Zhengcun Yan
    • Lun Dong
    • Lujun Pang
    • Xingdong Wang
  • View Affiliations

  • Published online on: August 8, 2016     https://doi.org/10.3892/mmr.2016.5598
  • Pages: 3134-3144
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Induced-resembled neuronal cells (irNCs) are generated by reprogramming human melanoma cells through the introduction of key transcription factors, providing novel concepts in the treatment of malignant tumor cells and making it possible to supply neural cells for laboratory use. In the present study, irNCs were derived from A375 cells by inducing the ‘forced’ overexpression of specific genes, including achaete‑scute homolog 1 (Ascl1), neuronal differentiation factor 1 (Neurod1), myelin transcription factor 1 (Myt1), brain protein 2 (Brn2, also termed POU3F2) and human brain‑derived neurotrophic factor (h‑BDNF). irNCs induced from A375 cells express multiple neuronal markers and fire action potentials, exhibiting properties similar to those of motor neurons. The reprogramming procedure comprised reverse transcription‑polymerase chain reaction and immunofluorescence staining; furthermore, electrophysiological profiling demonstrated the characteristics of the induced‑resembled neurons. The present study obtained a novel type of human irNC from human melanoma, which secreted BDNF continuously, providing a model for neuron‑like cells. Thus, irNCs offer promise in investigating various neural diseases by using neural‑like cells derived directly from the patient of interest.
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October-2016
Volume 14 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhang H, Wei M, Jiang Y, Wang X, She L, Yan Z, Dong L, Pang L and Wang X: Reprogramming A375 cells to induced‑resembled neuronal cells by structured overexpression of specific transcription genes. Mol Med Rep 14: 3134-3144, 2016
APA
Zhang, H., Wei, M., Jiang, Y., Wang, X., She, L., Yan, Z. ... Wang, X. (2016). Reprogramming A375 cells to induced‑resembled neuronal cells by structured overexpression of specific transcription genes. Molecular Medicine Reports, 14, 3134-3144. https://doi.org/10.3892/mmr.2016.5598
MLA
Zhang, H., Wei, M., Jiang, Y., Wang, X., She, L., Yan, Z., Dong, L., Pang, L., Wang, X."Reprogramming A375 cells to induced‑resembled neuronal cells by structured overexpression of specific transcription genes". Molecular Medicine Reports 14.4 (2016): 3134-3144.
Chicago
Zhang, H., Wei, M., Jiang, Y., Wang, X., She, L., Yan, Z., Dong, L., Pang, L., Wang, X."Reprogramming A375 cells to induced‑resembled neuronal cells by structured overexpression of specific transcription genes". Molecular Medicine Reports 14, no. 4 (2016): 3134-3144. https://doi.org/10.3892/mmr.2016.5598