Collagen regulates transforming growth factor-β receptors of HL-1 cardiomyocytes through activation of stretch and integrin signaling

  • Authors:
    • Yen‑Yu Lu
    • Yung‑Kuo Lin
    • Yu‑Hsun Kao
    • Cheng‑Chih Chung
    • Yung‑Hsin Yeh
    • Shih‑Ann Chen
    • Yi‑Jen Chen
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  • Published online on: August 18, 2016     https://doi.org/10.3892/mmr.2016.5635
  • Pages: 3429-3436
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Abstract

The extracellular matrix (ECM) and transforming growth factor-β (TGF)-β are important in cardiac fibrosis, however, the effects of the ECM on TGF‑β signaling remain to be fully elucidated. The aims of the present study were to evaluate the role of collagen in TGF‑β signaling and examine the underlying mechanisms. In the present study, western blot analysis was used to examine TGF‑β signaling in HL‑1 cells treated with and without (control) type I collagen (10 µg/ml), which was co‑administered with either an anti‑β1 integrin antibody (10 µg/ml) or a stretch‑activated channel inhibitor (gadolinium; 50 µM). Cell proliferation and adhesion assays were used to investigate the roles of integrin, mechanical stretch and mitogen‑activated protein kinases (MAPKs) on cell proliferation and adhesion. The type I collagen (10 µg/ml)‑treated HL‑1 cells were incubated with or without anti‑β1 integrin antibody (10 µg/ml), gadolinium (50 µM) or inhibitors of p38 (SB203580; 3 µM), extracellular signal‑regulated kinase (ERK; PD98059; 50 µM) and c‑Jun N‑terminal kinase (JNK; SP600125; 50 µM). Compared with the control cells, the collagen‑treated HL‑1 cells had lower expression levels of type I and type II TGF‑β receptors (TGFβRI and TGFβRII), with an increase in phosphorylated focal adhesion kinase (FAK), p38 and ERK1/2, and a decrease in JNK. Incubation with the anti‑β1 integrin antibody reversed the collagen‑induced downregulation of the expression of TGFβRII and phosphorylated FAK. Gadolinium downregulated the expression levels of TGFβRI and small mothers against decapentaplegic (Smad)2/3, and decreased the levels of phosphorylated p38, ERK1/2 and JNK. In addition, gadolinium reversed the collagen‑induced activation of p38 and ERK1/2. In the presence of gadolinium and anti‑β1 integrin antibody, collagen regulated the expression levels of TGFβRI, TGFβRII and Smad2/3, but did not alter the phosphorylation of p38, ERK1/2 or JNK. In addition, collagen increased cell proliferation and adhesion, and this collagen‑induced cell proliferation was inhibited by the anti‑β1 integrin antibody and ERK inhibitor. Taken together, the data obtained suggested that collagen differentially regulated the expression levels of TGFβRI and TGFβRII, and modulated the phosphorylation of MAPKs through integrin‑ or stretch‑dependent and ‑independent signaling pathways.
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October-2016
Volume 14 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Lu YY, Lin YK, Kao YH, Chung CC, Yeh YH, Chen SA and Chen YJ: Collagen regulates transforming growth factor-β receptors of HL-1 cardiomyocytes through activation of stretch and integrin signaling. Mol Med Rep 14: 3429-3436, 2016
APA
Lu, Y., Lin, Y., Kao, Y., Chung, C., Yeh, Y., Chen, S., & Chen, Y. (2016). Collagen regulates transforming growth factor-β receptors of HL-1 cardiomyocytes through activation of stretch and integrin signaling. Molecular Medicine Reports, 14, 3429-3436. https://doi.org/10.3892/mmr.2016.5635
MLA
Lu, Y., Lin, Y., Kao, Y., Chung, C., Yeh, Y., Chen, S., Chen, Y."Collagen regulates transforming growth factor-β receptors of HL-1 cardiomyocytes through activation of stretch and integrin signaling". Molecular Medicine Reports 14.4 (2016): 3429-3436.
Chicago
Lu, Y., Lin, Y., Kao, Y., Chung, C., Yeh, Y., Chen, S., Chen, Y."Collagen regulates transforming growth factor-β receptors of HL-1 cardiomyocytes through activation of stretch and integrin signaling". Molecular Medicine Reports 14, no. 4 (2016): 3429-3436. https://doi.org/10.3892/mmr.2016.5635