Open Access

Fibronectin expression is critical for liver fibrogenesis in vivo and in vitro

  • Authors:
    • Xiao‑Ya Liu
    • Rui‑Xia Liu
    • Fei Hou
    • Li‑Jian Cui
    • Chun-Yun Li
    • Cheng Chi
    • Entong Yi
    • Yan Wen
    • Cheng‑Hong Yin
  • View Affiliations

  • Published online on: August 25, 2016     https://doi.org/10.3892/mmr.2016.5673
  • Pages: 3669-3675
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Increased fibronectin (FN) expression has an important role during liver fibrosis. The present study examined FN expression in rats subjected to carbon tetrachloride (CCl4)‑induced liver fibrosis. In addition, the potential mechanisms underlying fibrogenesis were investigated by exposing hepatic stellate cells (HSCs) to transforming growth factor‑β (TGF‑β), which is a known inducer of myofibroblastic transformation of HSCs. Briefly, a rat model of liver fibrosis was created by administering intraperitoneal injections of CCl4. Furthermore, HSC‑T6 cells were stimulated with increasing doses of recombinant TGF‑β over 24 h. Hepatic fibrosis gradually increased following CCl4 administration in vivo. Western blotting and immunohistochemistry demonstrated that fibronectin (FN), TGF‑β and α‑smooth muscle actin (SMA) expression was increased following CCl4 injection, and the maximum expression levels were observed at 8 weeks. Once CCl4 treatment had been terminated, the expression levels of FN, TGF‑β and α‑SMA progressively declined to near baseline levels. Western blotting and quantitative polymerase chain reaction demonstrated that FN expression was gradually increased in response to TGF‑β‑stimulation of HSCs; maximum expression was achieved 12 h post‑treatment (P<0.01 vs. the baseline). In conclusion, these findings indicated that FN expression is an early and progressive event that occurs during liver fibrogenesis in vivo and in vitro.
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October-2016
Volume 14 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Liu XY, Liu RX, Hou F, Cui LJ, Li C, Chi C, Yi E, Wen Y and Yin CH: Fibronectin expression is critical for liver fibrogenesis in vivo and in vitro. Mol Med Rep 14: 3669-3675, 2016
APA
Liu, X., Liu, R., Hou, F., Cui, L., Li, C., Chi, C. ... Yin, C. (2016). Fibronectin expression is critical for liver fibrogenesis in vivo and in vitro. Molecular Medicine Reports, 14, 3669-3675. https://doi.org/10.3892/mmr.2016.5673
MLA
Liu, X., Liu, R., Hou, F., Cui, L., Li, C., Chi, C., Yi, E., Wen, Y., Yin, C."Fibronectin expression is critical for liver fibrogenesis in vivo and in vitro". Molecular Medicine Reports 14.4 (2016): 3669-3675.
Chicago
Liu, X., Liu, R., Hou, F., Cui, L., Li, C., Chi, C., Yi, E., Wen, Y., Yin, C."Fibronectin expression is critical for liver fibrogenesis in vivo and in vitro". Molecular Medicine Reports 14, no. 4 (2016): 3669-3675. https://doi.org/10.3892/mmr.2016.5673