Open Access

Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis

  • Authors:
    • Jinjiang Yang
    • Ying Lu
    • Ai Guo
  • View Affiliations

  • Published online on: September 23, 2016     https://doi.org/10.3892/mmr.2016.5767
  • Pages: 4075-4082
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Interleukin (IL)-1β-induced chondrocyte apoptosis is associated with the pathogenesis of arthritis. Platelet‑rich plasma (PRP), which is derived from the patient's own blood and contains numerous growth factors, has the potential for arthritis treatment. Therefore, the present study aimed to determine the effects of PRP on chondrocyte apoptosis, under IL‑1β‑induced pathological conditions. Chondrocytes isolated from the knee joint of Sprague Dawley rats were used in the present study. Cell viability was determined using the Cell Counting kit‑8 assay, cell apoptosis was evaluated by flow cytometry, and the expression of apoptosis‑, anabolism‑ and catabolism-associated genes were detected by quantitative polymerase chain reaction; protein expression was detected by western blot analysis. The results demonstrated that 10% PRP in the culture medium increased chondrocyte proliferation, whereas IL‑1β induced cell apoptosis. Treatment with PRP significantly attenuated cell apoptosis in IL‑1β‑treated chondrocytes, and altered apoptosis‑associated expression at the gene and protein level. Furthermore, treatment with PRP significantly reduced matrix metalloproteinase production and promoted anabolism of cartilage extracellular matrix under IL‑1β treatment. The present study demonstrated the protective effects of PRP on chondrocyte apoptosis and extracellular matrix anabolism, and provided scientific evidence to support the potential use of PRP as a promising therapeutic strategy for the treatment of arthritis.
View Figures
View References

Related Articles

Journal Cover

November-2016
Volume 14 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yang J, Lu Y and Guo A: Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis. Mol Med Rep 14: 4075-4082, 2016
APA
Yang, J., Lu, Y., & Guo, A. (2016). Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis. Molecular Medicine Reports, 14, 4075-4082. https://doi.org/10.3892/mmr.2016.5767
MLA
Yang, J., Lu, Y., Guo, A."Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis". Molecular Medicine Reports 14.5 (2016): 4075-4082.
Chicago
Yang, J., Lu, Y., Guo, A."Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis". Molecular Medicine Reports 14, no. 5 (2016): 4075-4082. https://doi.org/10.3892/mmr.2016.5767