Effects of polysaccharides isolated from Inonotus obliquus against hydrogen peroxide-induced oxidative damage in RINm5F pancreatic β-cells

  • Authors:
    • Ye Chan Sim
    • Jong Seok Lee
    • Sarah Lee
    • Youn Kyoung Son
    • Jung‑Eun Park
    • Jeong Eun Song
    • Suk‑Jin Ha
    • Eock Kee Hong
  • View Affiliations

  • Published online on: September 22, 2016     https://doi.org/10.3892/mmr.2016.5763
  • Pages: 4263-4270
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Abstract

The purpose of the present study was to elucidate the cytoprotective effects of polysaccharides isolated from Inonotus obliquus. The polysaccharides were extracted from the fruiting body of I. obliquus (PFIO) and the liquid culture broth of I. obliquus (PLIO). The effects of PFIO and PLIO on hydrogen peroxide (H2O2)‑induced oxidative damage of RINm5F pancreatic β‑cells were comparatively investigated using an MTT assay, immunofluorescent staining, flow cytometry, and western blot analyses in vitro. The results of the present study demonstrated that treatment with PFIO and PLIO decreased DNA fragmentation and the rate of apoptosis. In addition, pretreatment of cells with PFIO and PLIO prior to H2O2 exposure resulted in increased insulin secretion and scavenging activity for intracellular reactive oxygen species, as compared with treatment with H2O2 alone. The results of the present study suggested that PFIO and PLIO may exert protective effects against H2O2‑induced oxidative stress via the regulation of mitogen‑activated protein kinases, nuclear factor‑κB and apoptotic proteins. Therefore, PFIO and PLIO may have potential merit as a medicinal food for the prevention of diabetes.
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November-2016
Volume 14 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Sim YC, Lee JS, Lee S, Son YK, Park JE, Song JE, Ha SJ and Hong EK: Effects of polysaccharides isolated from Inonotus obliquus against hydrogen peroxide-induced oxidative damage in RINm5F pancreatic β-cells. Mol Med Rep 14: 4263-4270, 2016
APA
Sim, Y.C., Lee, J.S., Lee, S., Son, Y.K., Park, J., Song, J.E. ... Hong, E.K. (2016). Effects of polysaccharides isolated from Inonotus obliquus against hydrogen peroxide-induced oxidative damage in RINm5F pancreatic β-cells. Molecular Medicine Reports, 14, 4263-4270. https://doi.org/10.3892/mmr.2016.5763
MLA
Sim, Y. C., Lee, J. S., Lee, S., Son, Y. K., Park, J., Song, J. E., Ha, S., Hong, E. K."Effects of polysaccharides isolated from Inonotus obliquus against hydrogen peroxide-induced oxidative damage in RINm5F pancreatic β-cells". Molecular Medicine Reports 14.5 (2016): 4263-4270.
Chicago
Sim, Y. C., Lee, J. S., Lee, S., Son, Y. K., Park, J., Song, J. E., Ha, S., Hong, E. K."Effects of polysaccharides isolated from Inonotus obliquus against hydrogen peroxide-induced oxidative damage in RINm5F pancreatic β-cells". Molecular Medicine Reports 14, no. 5 (2016): 4263-4270. https://doi.org/10.3892/mmr.2016.5763