Identification of differentially expressed transcripts targeted by the knockdown of endogenous IFITM3

  • Authors:
    • Chen Shen
    • Ying‑Jia Li
    • Qing‑Qin Yin
    • Wei‑Wei Jiao
    • Qin‑Jing Li
    • Jing Xiao
    • Lin Sun
    • Fang Xu
    • Jie‑Qiong Li
    • Hui Qi
    • A‑Dong Shen
  • View Affiliations

  • Published online on: September 26, 2016     https://doi.org/10.3892/mmr.2016.5777
  • Pages: 4367-4373
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Abstract

Interferon inducible transmembrane protein 3 (IFITM3) is a double transmembrane protein. As a member of the IFITM family, IFITM3 can be upregulated by interferon (IFN) to be involved in various biological processes. In order to determine whether gene expression profiles can be altered by a lack of IFITM3, the present study used shRNAs lentivirus for knocking down the endogenous expression of IFITM3 in human HeLa cells and human whole genome microarrays to obtain gene expression profiles. A total of 1,011 downregulated transcripts and 615 upregulated transcripts were identified using the Agilent expression platform. The identified transcripts were involved in multiple pathways, including the complement pathways, and the antigen processing and presentation pathway. The present study identified the transcripts, which were affected by the downregulation of endogenous IFITM3 and the pathways they were involved in. These findings may lead to an improved understanding of the biological functions of IFITM3.
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November-2016
Volume 14 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Shen C, Li YJ, Yin QQ, Jiao WW, Li QJ, Xiao J, Sun L, Xu F, Li JQ, Qi H, Qi H, et al: Identification of differentially expressed transcripts targeted by the knockdown of endogenous IFITM3. Mol Med Rep 14: 4367-4373, 2016
APA
Shen, C., Li, Y., Yin, Q., Jiao, W., Li, Q., Xiao, J. ... Shen, A. (2016). Identification of differentially expressed transcripts targeted by the knockdown of endogenous IFITM3. Molecular Medicine Reports, 14, 4367-4373. https://doi.org/10.3892/mmr.2016.5777
MLA
Shen, C., Li, Y., Yin, Q., Jiao, W., Li, Q., Xiao, J., Sun, L., Xu, F., Li, J., Qi, H., Shen, A."Identification of differentially expressed transcripts targeted by the knockdown of endogenous IFITM3". Molecular Medicine Reports 14.5 (2016): 4367-4373.
Chicago
Shen, C., Li, Y., Yin, Q., Jiao, W., Li, Q., Xiao, J., Sun, L., Xu, F., Li, J., Qi, H., Shen, A."Identification of differentially expressed transcripts targeted by the knockdown of endogenous IFITM3". Molecular Medicine Reports 14, no. 5 (2016): 4367-4373. https://doi.org/10.3892/mmr.2016.5777