Open Access

NFAT5 participates in seawater inhalation‑induced acute lung injury via modulation of NF-κB activity

  • Authors:
    • Congcong Li
    • Manling Liu
    • Liyan Bo
    • Wei Liu
    • Qingqing Liu
    • Xiangjun Chen
    • Dunquan Xu
    • Zhichao Li
    • Faguang Jin
  • View Affiliations

  • Published online on: October 19, 2016     https://doi.org/10.3892/mmr.2016.5860
  • Pages: 5033-5040
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Nuclear factor of activated T cells 5 (NFAT5) is a transcription factor that can be activated by extracellular tonicity. It has been reported that NFAT5 may increase the transcription of certain osmoprotective genes in the renal system, and the aim of the current study was to explore the role of NFAT5 in seawater inhalation‑induced acute lung injury. Though establishing the model of seawater inhalation‑induced acute lung injury, it was demonstrated that seawater inhalation enhanced the transcription and protein expression of NFAT5 (evaluated by reverse transcription‑polymerase chain reaction, immunohistochemistry stain and western blotting) and activation of nuclear factor (NF)‑κB (evaluated by western blotting and mRNA expression levels of three NF‑κB‑dependent genes) both in lung tissue and rat alveolar macrophage cells (NR8383 cells). When expression of NFAT5 was reduced in NR8383 cells using an siRNA targeted to NFAT5, the phosphorylation of NF‑κB and transcription of NF‑κB‑dependent genes were significantly reduced. In addition, the elevated content of certain inflammatory cytokines [tumor necrosis factor α, interleukin (IL)‑1 and IL‑8] were markedly reduced. In conclusion, NFAT5 serves an important pathophysiological role in seawater inhalation‑induced acute lung injury by modulating NF‑κB activity, and these data suggest that NFAT5 may be a promising therapeutic target.
View Figures
View References

Related Articles

Journal Cover

December-2016
Volume 14 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Li C, Liu M, Bo L, Liu W, Liu Q, Chen X, Xu D, Li Z and Jin F: NFAT5 participates in seawater inhalation‑induced acute lung injury via modulation of NF-κB activity. Mol Med Rep 14: 5033-5040, 2016
APA
Li, C., Liu, M., Bo, L., Liu, W., Liu, Q., Chen, X. ... Jin, F. (2016). NFAT5 participates in seawater inhalation‑induced acute lung injury via modulation of NF-κB activity. Molecular Medicine Reports, 14, 5033-5040. https://doi.org/10.3892/mmr.2016.5860
MLA
Li, C., Liu, M., Bo, L., Liu, W., Liu, Q., Chen, X., Xu, D., Li, Z., Jin, F."NFAT5 participates in seawater inhalation‑induced acute lung injury via modulation of NF-κB activity". Molecular Medicine Reports 14.6 (2016): 5033-5040.
Chicago
Li, C., Liu, M., Bo, L., Liu, W., Liu, Q., Chen, X., Xu, D., Li, Z., Jin, F."NFAT5 participates in seawater inhalation‑induced acute lung injury via modulation of NF-κB activity". Molecular Medicine Reports 14, no. 6 (2016): 5033-5040. https://doi.org/10.3892/mmr.2016.5860