Open Access

Inhibitory effects of dieckol on hypoxia-induced epithelial-mesenchymal transition of HT29 human colorectal cancer cells

  • Authors:
    • Seung‑Hyun Jeong
    • You‑Jin Jeon
    • Sun Joo Park
  • View Affiliations

  • Published online on: October 21, 2016     https://doi.org/10.3892/mmr.2016.5872
  • Pages: 5148-5154
  • Copyright: © Jeong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hypoxia-induced epithelial-mesenchymal transition (EMT) has been identified as essential for tumor progression and metastasis. The present study examined the effects of an antioxidant, dieckol, on hypoxia‑induced EMT in HT29 human colorectal cancer cells. HT29 cells were treated with a hypoxia‑inducing agent, CoCl2, and an increase in the levels of intracellular reactive oxygen species (ROS) and various morphological changes, such as loss of cell‑cell contact and aggressive cell migration were observed. CoCl2 also induced an increase in the expression of hypoxia‑inducible factor 1α (HIF1α) and various mesenchymal‑specific markers, including vimentin and snail family transcriptional repressor 1 (Snail1), and a decrease in the expression of E‑cadherin, thus suggesting that CoCl2 induced EMT in HT29 cells. Conversely, the CoCl2‑induced EMT of HT29 cells was suppressed following treatment with dieckol. In addition, ROS generation, EMT marker protein expression and intracellular localization, cell migration and cell invasion were attenuated following dieckol treatment. The findings of the present study suggested that dieckol may inhibit hypoxia‑induced EMT in HT29 cells by regulating the levels of cellular ROS and protein expression levels downstream of the HIF1α signaling pathway. Therefore, dieckol has the potential to become an attractive therapeutic agent for the treatment of colorectal cancer.
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December-2016
Volume 14 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Jeong SH, Jeon YJ and Park SJ: Inhibitory effects of dieckol on hypoxia-induced epithelial-mesenchymal transition of HT29 human colorectal cancer cells. Mol Med Rep 14: 5148-5154, 2016
APA
Jeong, S., Jeon, Y., & Park, S.J. (2016). Inhibitory effects of dieckol on hypoxia-induced epithelial-mesenchymal transition of HT29 human colorectal cancer cells. Molecular Medicine Reports, 14, 5148-5154. https://doi.org/10.3892/mmr.2016.5872
MLA
Jeong, S., Jeon, Y., Park, S. J."Inhibitory effects of dieckol on hypoxia-induced epithelial-mesenchymal transition of HT29 human colorectal cancer cells". Molecular Medicine Reports 14.6 (2016): 5148-5154.
Chicago
Jeong, S., Jeon, Y., Park, S. J."Inhibitory effects of dieckol on hypoxia-induced epithelial-mesenchymal transition of HT29 human colorectal cancer cells". Molecular Medicine Reports 14, no. 6 (2016): 5148-5154. https://doi.org/10.3892/mmr.2016.5872