Mechanisms underlying the antiapoptotic and anti-inflammatory effects of monotropein in hydrogen peroxide-treated osteoblasts

  • Authors:
    • Fang‑Bing Zhu
    • Jian‑Yue Wang
    • Ying‑Liang Zhang
    • Yun‑Gen Hu
    • Zhen‑Shuang Yue
    • Lin‑Ru Zeng
    • Wen‑Jie Zheng
    • Qiao Hou
    • Shi‑Gui Yan
    • Ren‑Fu Quan
  • View Affiliations

  • Published online on: October 31, 2016     https://doi.org/10.3892/mmr.2016.5908
  • Pages: 5377-5384
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Abstract

Monotropein, the primary iridoid glycoside isolated from Morindacitrifolia, has been previously reported to possess potent antioxidant and antiosteoporotic properties. However, there is no direct evidence correlating the antiosteoporotic effect of monotropein with its observed antioxidant capacity, and the molecular mechanisms involved in mediating these processes remain unclear. Therefore, the aim of the present study was to investigate the protective effects of monotropein against oxidative stress in osteoblasts and the mechanisms involved in mediating this process. Osteoblast viability was evaluated using the MTT assay. The mitochondrial membrane potential and reactive oxygen species were detected by flow cytometry analyses. Western blotting and enzyme‑linked immunosorbent assays were performed to detect protein expression levels. A significant reduction in osteoblast viability was observed at 24 h following exposure to various concentrations (100‑1,000 µM) of H2O2 compared with untreated osteoblasts. The cytotoxic effect of H2O2 was notably reversed when osteoblasts were pretreated with 1‑10 µg/ml monotropein. Pretreatment with 1-10 µg/ml monotropein increased the mitochondrial membrane potential and reduced the generation of reactive oxygen species in osteoblasts following exposure to H2O2. In addition, the H2O2‑induced increase in apoptotic markers (caspase-3 and caspase-9) and H2O2-induced reduction in sirtuin 1 levels were significantly reversed following pretreatment of cells with monotropein. Furthermore, monotropein significantly reduced H2O2‑induced stimulation of NF‑κB expression, in addition to the expression of a number of proinflammatory mediators. These results indicate that monotropein suppresses apoptosis and the inflammatory response in H2O2‑induced osteoblasts through the activation of the mitochondrial apoptotic signaling pathway and inhibition of the NF‑κB signaling pathway.
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December-2016
Volume 14 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhu FB, Wang JY, Zhang YL, Hu YG, Yue ZS, Zeng LR, Zheng WJ, Hou Q, Yan SG, Quan RF, Quan RF, et al: Mechanisms underlying the antiapoptotic and anti-inflammatory effects of monotropein in hydrogen peroxide-treated osteoblasts. Mol Med Rep 14: 5377-5384, 2016
APA
Zhu, F., Wang, J., Zhang, Y., Hu, Y., Yue, Z., Zeng, L. ... Quan, R. (2016). Mechanisms underlying the antiapoptotic and anti-inflammatory effects of monotropein in hydrogen peroxide-treated osteoblasts. Molecular Medicine Reports, 14, 5377-5384. https://doi.org/10.3892/mmr.2016.5908
MLA
Zhu, F., Wang, J., Zhang, Y., Hu, Y., Yue, Z., Zeng, L., Zheng, W., Hou, Q., Yan, S., Quan, R."Mechanisms underlying the antiapoptotic and anti-inflammatory effects of monotropein in hydrogen peroxide-treated osteoblasts". Molecular Medicine Reports 14.6 (2016): 5377-5384.
Chicago
Zhu, F., Wang, J., Zhang, Y., Hu, Y., Yue, Z., Zeng, L., Zheng, W., Hou, Q., Yan, S., Quan, R."Mechanisms underlying the antiapoptotic and anti-inflammatory effects of monotropein in hydrogen peroxide-treated osteoblasts". Molecular Medicine Reports 14, no. 6 (2016): 5377-5384. https://doi.org/10.3892/mmr.2016.5908