Open Access

Rapid and sensitive detection of Plesiomonas shigelloides by cross‑priming amplification of the hugA gene

  • Authors:
    • Shuang Meng
    • Yi Wang
    • Yan Wang
    • Changyun Ye
  • View Affiliations

  • Published online on: November 14, 2016     https://doi.org/10.3892/mmr.2016.5937
  • Pages: 5443-5450
  • Copyright: © Meng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Plesiomonas shigelloides (P. shigelloides) is implicated as an aetiological agent of human gastroenteritis in humans, for which reliable laboratory detection of P. shigelloides is clinically and epidemiologically desirable. A simple molecular method for rapid detection of P. shigelloides using cross‑priming amplification (CPA) has been developed, with hugA as the target. The hugA gene is required for haem iron utilisation and is critical for the survival and growth of P. shigelloides. The assay output was visualised as a colour change with no need to open the reaction tubes, and no false‑positive results were detected for the 33 non‑ P. shigelloides strains examined to assess assay specificity. The limit of detection was 200 fg P. shigelloides DNA per reaction and 3x103 CFU per g in human stools, which was 100 and 10‑fold more sensitive than polymerase chain reaction, respectively. The CPA method was used to detect the presence of P. shigelloides in stool specimens from 70 patients with diarrhoea and 30 environmental water samples, with no difference in accuracy between the CPA assay and the biological culture. The present study, therefore, suggests that the P. shigelloides hugA CPA assay may represent a valuable tool for rapid and sensitive detection of P. shigelloides in primary care facilities and clinical laboratories.
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December-2016
Volume 14 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Meng S, Wang Y, Wang Y and Ye C: Rapid and sensitive detection of Plesiomonas shigelloides by cross‑priming amplification of the hugA gene. Mol Med Rep 14: 5443-5450, 2016
APA
Meng, S., Wang, Y., Wang, Y., & Ye, C. (2016). Rapid and sensitive detection of Plesiomonas shigelloides by cross‑priming amplification of the hugA gene. Molecular Medicine Reports, 14, 5443-5450. https://doi.org/10.3892/mmr.2016.5937
MLA
Meng, S., Wang, Y., Wang, Y., Ye, C."Rapid and sensitive detection of Plesiomonas shigelloides by cross‑priming amplification of the hugA gene". Molecular Medicine Reports 14.6 (2016): 5443-5450.
Chicago
Meng, S., Wang, Y., Wang, Y., Ye, C."Rapid and sensitive detection of Plesiomonas shigelloides by cross‑priming amplification of the hugA gene". Molecular Medicine Reports 14, no. 6 (2016): 5443-5450. https://doi.org/10.3892/mmr.2016.5937