Alteration of epoxyeicosatrienoic acids in the liver and kidney of cytochrome P450 4F2 transgenic mice

  • Authors:
    • Bijun Zhang
    • Guangrui Lai
    • Xiaoliang Liu
    • Yanyan Zhao
  • View Affiliations

  • Published online on: November 23, 2016     https://doi.org/10.3892/mmr.2016.5962
  • Pages: 5739-5745
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Abstract

Arachidonic acid (AA) can be metabolized into 20-hydroxyeicosatetraenoic acid (20-HETE) by ω-hydroxylases, and epoxyeicosatrienoic acids (EETs) by epoxygenases. The effects of EETs in cardiovascular physiology are vasodilatory, anti-inflammatory and anti‑apoptotic, which are opposite to the function to 20‑HETE. However, EETs are not stable in vivo, and are rapidly degraded to the biologically less active metabolites, dihydroxyeicosatrienoic acids, via soluble epoxide hydrolase (sEH). Western blotting, reverse transcription‑quantitative polymerase chain reaction and liquid chromatography tandem mass spectrometry were performed in order to determine target RNA and protein expression levels. In the present study, it was demonstrated that the disturbed renal 20‑HETE/EET ratio in the hypertensive cytochrome P450 4F2 transgenic mice was caused by the activation of sEH and the repression of epoxygenase activity. In addition, 20‑HETE showed an opposite regulatory effect on the endogenous epoxygenases in the liver and kidney. Given that 20‑HETE and EETs have opposite effects in multiple disease, the regulation of their formation and degradation may yield therapeutic benefits.
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December-2016
Volume 14 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhang B, Lai G, Liu X and Zhao Y: Alteration of epoxyeicosatrienoic acids in the liver and kidney of cytochrome P450 4F2 transgenic mice. Mol Med Rep 14: 5739-5745, 2016
APA
Zhang, B., Lai, G., Liu, X., & Zhao, Y. (2016). Alteration of epoxyeicosatrienoic acids in the liver and kidney of cytochrome P450 4F2 transgenic mice. Molecular Medicine Reports, 14, 5739-5745. https://doi.org/10.3892/mmr.2016.5962
MLA
Zhang, B., Lai, G., Liu, X., Zhao, Y."Alteration of epoxyeicosatrienoic acids in the liver and kidney of cytochrome P450 4F2 transgenic mice". Molecular Medicine Reports 14.6 (2016): 5739-5745.
Chicago
Zhang, B., Lai, G., Liu, X., Zhao, Y."Alteration of epoxyeicosatrienoic acids in the liver and kidney of cytochrome P450 4F2 transgenic mice". Molecular Medicine Reports 14, no. 6 (2016): 5739-5745. https://doi.org/10.3892/mmr.2016.5962