Involvement of retinoblastoma-associated protein 48 during photodynamic therapy of cervical cancer cells

  • Authors:
    • Shuxia Wu
    • Lijun Wang
    • Xingye Ren
    • Yulu Pan
    • Yan Peng
    • Xiaoyan Zou
    • Cuige Shi
    • Youzhong Zhang
  • View Affiliations

  • Published online on: January 26, 2017     https://doi.org/10.3892/mmr.2017.6151
  • Pages: 1393-1400
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Abstract

5-Aminolevulinic acid-mediated photodynamic therapy (ALA‑PDT) is an effective treatment option for cervical intraepithelial neoplasia, the precancerous lesion of cervical cancer, and early cervical cancer, particularly for young or nulliparous women who want to remain fertile. A previous report described the involvement of histone deacetylases (HDAC) during ALA‑PDT mediated apoptosis in the cerebral cortex of a mouse model. Retinoblastoma‑associated protein 48 (RbAp48), a highly abundant component of HDACs, is a critical mediator that controls the transforming activity of human papillomavirus 16 in cervical cancer cells. The aim of the present study was to investigate the involvement of RbAp48 in ALA‑PDT‑induced cell death in cervical cancer cells. RbAp48 was significantly upregulated in cervical cancer cell lines treated with ALA‑PDT, including SiHa and HeLa cells. To establish the relevance of RbAp48 and the efficacy of ALA‑PDT in cervical cancer cells, the effect of ALA‑PDT was investigated in SiHa or HeLa cells following the depletion of RbAp48 by small interfering RNA (siRNA). Reduction of RbAp48 led to the reduced suppression of proliferation and apoptosis induced by ALA‑PDT in cervical cancer cells, which was associated with a reduction in tumor suppressor protein 53 (p53), retinoblastoma (Rb), apoptosis‑related enzyme caspase‑3, and increased levels of the oncogenic genes, human papillomavirus E6 and E7. These results provide evidence that RbAp48 is an important contributor to the efficacy of ALA‑PDT in cervical cancer cells. RbAp48 may be a therapeutic target that may help to improve the treatment of cervical cancer.
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March-2017
Volume 15 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wu S, Wang L, Ren X, Pan Y, Peng Y, Zou X, Shi C and Zhang Y: Involvement of retinoblastoma-associated protein 48 during photodynamic therapy of cervical cancer cells. Mol Med Rep 15: 1393-1400, 2017
APA
Wu, S., Wang, L., Ren, X., Pan, Y., Peng, Y., Zou, X. ... Zhang, Y. (2017). Involvement of retinoblastoma-associated protein 48 during photodynamic therapy of cervical cancer cells. Molecular Medicine Reports, 15, 1393-1400. https://doi.org/10.3892/mmr.2017.6151
MLA
Wu, S., Wang, L., Ren, X., Pan, Y., Peng, Y., Zou, X., Shi, C., Zhang, Y."Involvement of retinoblastoma-associated protein 48 during photodynamic therapy of cervical cancer cells". Molecular Medicine Reports 15.3 (2017): 1393-1400.
Chicago
Wu, S., Wang, L., Ren, X., Pan, Y., Peng, Y., Zou, X., Shi, C., Zhang, Y."Involvement of retinoblastoma-associated protein 48 during photodynamic therapy of cervical cancer cells". Molecular Medicine Reports 15, no. 3 (2017): 1393-1400. https://doi.org/10.3892/mmr.2017.6151