Open Access

Expression of TGF-β1/mTOR signaling pathway in pathological scar fibroblasts

  • Authors:
    • Xiao‑Xiang Zhai
    • Zhi‑Ming Tang
    • Ji‑Cun Ding
    • Xiao‑Lan Lu
  • View Affiliations

  • Published online on: April 5, 2017     https://doi.org/10.3892/mmr.2017.6437
  • Pages: 3467-3472
  • Copyright: © Zhai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the present study was to detect the expression of the key molecules, including transforming growth factor‑β1 (TGF-β1), phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt) of TGF‑β1/mammalian target of rapamycin (mTOR) pathway in pathological scar fibroblasts. Immunofluorescence, reverse transcription‑polymerase chain reaction (RT‑PCR) and western blot analysis were used to detect the expression of the key molecules TGF‑β1, PI3K, Akt, mTOR in fibroblasts of normal skin tissue and pathological scar tissue. Immunofluorescence showed that the expression of TGF‑β1, PI3K and Akt was significantly enhanced (P<0.05) in pathological scar fibroblasts, and mainly expressed in the cell nucleus, but not in normal skin tissue or fibroblasts. RT‑PCR and western blot test results revealed that the TGF‑β1, PI3K, Akt, and mTOR mRNA and protein expression in pathological scar fibroblasts were significantly higher (P<0.05) than in the normal skin tissue. Expression of the TGF‑β1/mTOR signaling pathway in pathological scar fibroblasts was significantly increased. Data suggest that this expression may be an important mechanism for pathological scar formation.
View Figures
View References

Related Articles

Journal Cover

June-2017
Volume 15 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhai XX, Tang ZM, Ding JC and Lu XL: Expression of TGF-β1/mTOR signaling pathway in pathological scar fibroblasts. Mol Med Rep 15: 3467-3472, 2017
APA
Zhai, X., Tang, Z., Ding, J., & Lu, X. (2017). Expression of TGF-β1/mTOR signaling pathway in pathological scar fibroblasts. Molecular Medicine Reports, 15, 3467-3472. https://doi.org/10.3892/mmr.2017.6437
MLA
Zhai, X., Tang, Z., Ding, J., Lu, X."Expression of TGF-β1/mTOR signaling pathway in pathological scar fibroblasts". Molecular Medicine Reports 15.6 (2017): 3467-3472.
Chicago
Zhai, X., Tang, Z., Ding, J., Lu, X."Expression of TGF-β1/mTOR signaling pathway in pathological scar fibroblasts". Molecular Medicine Reports 15, no. 6 (2017): 3467-3472. https://doi.org/10.3892/mmr.2017.6437