Open Access

Protein therapy using MafA fused to a polyarginine transduction domain attenuates glucose levels of streptozotocin‑induced diabetic mice

  • Authors:
    • Jun Lu
    • Lingjing Lin
    • Huiyue Dong
    • Xin Meng
    • Fang Fang
    • Qinghua Wang
    • Lianghu Huang
    • Jianming Tan
  • View Affiliations

  • Published online on: May 2, 2017     https://doi.org/10.3892/mmr.2017.6536
  • Pages: 4041-4048
  • Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ectopic expression of musculo aponeurotic fibrosarcoma BZIP transcription factor (Maf) A, has previously been demonstrated to induce insulin expression in non‑β‑cell lines. Protein transduction domains acting as an alternative delivery strategy may deliver heterogeneous proteins into cells. A sequence of 11 arginine residues (11R) has been demonstrated to act as a particularly efficient vector to introduce proteins into various cell types. The present study constructed 11R‑fused MafA to achieve transduction of the protein into cellular membranes and subsequently examined the therapeutic effect of the MafA‑11R protein in streptozotocin‑induced diabetes. A small animal imaging system was used to demonstrate that 11R introduced proteins into cells. The MafA‑11R protein was then injected into the tale vein of healthy male mice, and western blot analysis and immunofluorescence staining was performed to identify the location of the recombinant protein. Ameliorated hyperglycemia in the MafA‑11R‑treated diabetic mice was demonstrated via the improved intraperitoneal glucose tolerance test (IPGTT) and glucose‑stimulated insulin release. Furthermore, insulin producing cells were detected in the jejunum of the MafA‑11R treated mice. The results of the present study indicated that MafA‑11R delivery may act as a novel and potential therapeutic strategy for the future and will not present adverse effects associated with viral vector‑mediated gene therapies.
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June-2017
Volume 15 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Lu J, Lin L, Dong H, Meng X, Fang F, Wang Q, Huang L and Tan J: Protein therapy using MafA fused to a polyarginine transduction domain attenuates glucose levels of streptozotocin‑induced diabetic mice. Mol Med Rep 15: 4041-4048, 2017
APA
Lu, J., Lin, L., Dong, H., Meng, X., Fang, F., Wang, Q. ... Tan, J. (2017). Protein therapy using MafA fused to a polyarginine transduction domain attenuates glucose levels of streptozotocin‑induced diabetic mice. Molecular Medicine Reports, 15, 4041-4048. https://doi.org/10.3892/mmr.2017.6536
MLA
Lu, J., Lin, L., Dong, H., Meng, X., Fang, F., Wang, Q., Huang, L., Tan, J."Protein therapy using MafA fused to a polyarginine transduction domain attenuates glucose levels of streptozotocin‑induced diabetic mice". Molecular Medicine Reports 15.6 (2017): 4041-4048.
Chicago
Lu, J., Lin, L., Dong, H., Meng, X., Fang, F., Wang, Q., Huang, L., Tan, J."Protein therapy using MafA fused to a polyarginine transduction domain attenuates glucose levels of streptozotocin‑induced diabetic mice". Molecular Medicine Reports 15, no. 6 (2017): 4041-4048. https://doi.org/10.3892/mmr.2017.6536