Effects of unfractionated heparin and rivaroxaban on the expression of heparanase and fibroblast growth factor 2 in human osteoblasts

  • Authors:
    • Jingjun Xia
    • Wei Sheng
    • Liubao Pei
    • Ning Li
    • Zhipeng Zhang
    • Jialiang Wang
    • Jianing Zu
    • Nanxiang Wang
    • Duanyang Wang
  • View Affiliations

  • Published online on: May 10, 2017     https://doi.org/10.3892/mmr.2017.6570
  • Pages: 361-366
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Long-term treatment with anticoagulants may contribute to osteoporosis. Although unfractionated heparin and rivaroxaban have adverse effects on bone microstructure and function in adult rats, the underlying mechanism remains to be elucidated. Heparanase (HPSE) and fibroblast growth factor (FGF)2 are important signals in bone formation and fracture healing. Therefore, the present study was designed to investigate the effects of unfractionated heparin and rivaroxaban on the expression of HPSE and FGF2 in human osteoblasts. Human osteoblasts were treated with unfractionated heparin (0.5-50 IU/ml) or rivaroxaban (0.13‑13 µg/ml) for different durations. Plasmids encoding HPSE and FGF2 were transfected into osteoblasts, and cell viability was assessed using MTT assays, with mRNA and protein expression levels determined using reverse transcription‑quantitative polymerase chain reaction and western blot analyses, respectively. Osteoblast growth was significantly inhibited by treatment with unfractionated heparin (50 IU/ml) or rivaroxaban (13 µg/ml). Unfractionated heparin alone significantly inhibited the expression of HPSE and FGF2, whereas rivaroxaban inhibited the expression of FGF2 without affecting that of HPSE. Furthermore, the overexpression of HPSE or FGF2 significantly reversed the inhibitory effects of unfractionated heparin and rivaroxaban on osteoblasts. These findings suggested that HPSE and FGF2 signals were involved in the detrimental role of unfractionated heparin and rivaroxaban in human osteoblasts, providing novel information on the side effects of anticoagulants.
View Figures
View References

Related Articles

Journal Cover

July-2017
Volume 16 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Xia J, Sheng W, Pei L, Li N, Zhang Z, Wang J, Zu J, Wang N and Wang D: Effects of unfractionated heparin and rivaroxaban on the expression of heparanase and fibroblast growth factor 2 in human osteoblasts. Mol Med Rep 16: 361-366, 2017
APA
Xia, J., Sheng, W., Pei, L., Li, N., Zhang, Z., Wang, J. ... Wang, D. (2017). Effects of unfractionated heparin and rivaroxaban on the expression of heparanase and fibroblast growth factor 2 in human osteoblasts. Molecular Medicine Reports, 16, 361-366. https://doi.org/10.3892/mmr.2017.6570
MLA
Xia, J., Sheng, W., Pei, L., Li, N., Zhang, Z., Wang, J., Zu, J., Wang, N., Wang, D."Effects of unfractionated heparin and rivaroxaban on the expression of heparanase and fibroblast growth factor 2 in human osteoblasts". Molecular Medicine Reports 16.1 (2017): 361-366.
Chicago
Xia, J., Sheng, W., Pei, L., Li, N., Zhang, Z., Wang, J., Zu, J., Wang, N., Wang, D."Effects of unfractionated heparin and rivaroxaban on the expression of heparanase and fibroblast growth factor 2 in human osteoblasts". Molecular Medicine Reports 16, no. 1 (2017): 361-366. https://doi.org/10.3892/mmr.2017.6570