Open Access

Effects of demethoxycurcumin on the viability and apoptosis of skin cancer cells

  • Authors:
    • Yaoqun Wu
    • Pei Zhang
    • Hongyun Yang
    • Yong Ge
    • Yong Xin
  • View Affiliations

  • Published online on: May 31, 2017     https://doi.org/10.3892/mmr.2017.6666
  • Pages: 539-546
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study investigated the effects and mechanisms of demethoxycurcumin (DMC) on a human skin squamous cell carcinoma cell line, A431, and a human keratinocyte cell line, HaCaT. A431 and HaCaT cells were cultured in vitro. The effects of DMC treatment on cell viability were analyzed using the Cell Counting kit‑8 (CCK‑8) assay; cell cycle distribution was analyzed by flow cytometry; apoptosis was assessed by flow cytometry and Hoechst 33258 staining; and the protein expression levels of cytochrome c, B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein (BAX), caspase‑9 and caspase‑3 were evaluated by western blotting. CCK‑8 assay results demonstrated that DMC treatment significantly inhibited viability of A431 and HaCaT cells in a dose‑dependent manner. Flow cytometric analysis confirmed that DMC treatment induced apoptosis in a dose‑dependent manner, and significantly increased the proportion of cells in G2/M phase. Western blot analysis indicated that the protein expression levels of Bcl‑2 were decreased, whereas the expression levels of BAX, caspase‑9, caspase‑3 and cytochrome c were increased following DMC treatment compared with in untreated cells. In conclusion, DMC treatment significantly inhibited viability of A431 and HaCaT cells, and induced cell cycle arrest in G2/M phase. The present study indicated that DMC may induce apoptosis of skin cancer cells through a caspase‑dependent pathway.
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July-2017
Volume 16 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wu Y, Zhang P, Yang H, Ge Y and Xin Y: Effects of demethoxycurcumin on the viability and apoptosis of skin cancer cells. Mol Med Rep 16: 539-546, 2017
APA
Wu, Y., Zhang, P., Yang, H., Ge, Y., & Xin, Y. (2017). Effects of demethoxycurcumin on the viability and apoptosis of skin cancer cells. Molecular Medicine Reports, 16, 539-546. https://doi.org/10.3892/mmr.2017.6666
MLA
Wu, Y., Zhang, P., Yang, H., Ge, Y., Xin, Y."Effects of demethoxycurcumin on the viability and apoptosis of skin cancer cells". Molecular Medicine Reports 16.1 (2017): 539-546.
Chicago
Wu, Y., Zhang, P., Yang, H., Ge, Y., Xin, Y."Effects of demethoxycurcumin on the viability and apoptosis of skin cancer cells". Molecular Medicine Reports 16, no. 1 (2017): 539-546. https://doi.org/10.3892/mmr.2017.6666