Open Access

Prostaglandin E2 promotes human CD34+ cells homing through EP2 and EP4 in vitro

  • Authors:
    • Yaqun Wang
    • Shuping Lai
    • Jing Tang
    • Chun Feng
    • Fangjie Liu
    • Chang Su
    • Waiyi Zou
    • Huizhen Chen
    • Duorong Xu
  • View Affiliations

  • Published online on: May 30, 2017     https://doi.org/10.3892/mmr.2017.6649
  • Pages: 639-646
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Recently, certain studies have demonstrated in vitro that prostaglandin E2 (PGE2) promotes human cluster of differentiation (CD)34+ cell homing. However, the sub‑type receptors activated by PGE2 are unknown, as the PGE2 receptor EP1-4 subtypes (EP1-4) are expressed on the membrane of human CD34+ cells. Based on the above, the present study aimed to screen the receptor subtype activity by PGE2 to promote human CD34+ cell homing. It was observed that human CD34+ cells expressed the four PGE2 sub‑receptors, particularly EP2 and 4. PGE2 increased EP2 and 4 mRNA expression significantly, while EP1 and 3 mRNA exhibited no significant alteration. PGE2, EP2 agonist (EP2A), and EP4A upregulated C‑X‑C chemokine receptor 4 mRNA and protein expression in human CD34+ cells, and promoted stromal cell‑derived factor 1α (SDF‑1α) expression in bone marrow mesenchymal stem cells (BMMSCs). These phenomena were inhibited by the associated receptor antagonists. PGE2, EP2A, and EP4A facilitated human CD34+ cell migration towards SDF‑1α and BMMSCs. The results of the present study suggested that PGE2 promoted human CD34+ cell homing through EP2 and 4 receptors in vitro.
View Figures
View References

Related Articles

Journal Cover

July-2017
Volume 16 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Lai S, Tang J, Feng C, Liu F, Su C, Zou W, Chen H and Xu D: Prostaglandin E2 promotes human CD34+ cells homing through EP2 and EP4 in vitro. Mol Med Rep 16: 639-646, 2017
APA
Wang, Y., Lai, S., Tang, J., Feng, C., Liu, F., Su, C. ... Xu, D. (2017). Prostaglandin E2 promotes human CD34+ cells homing through EP2 and EP4 in vitro. Molecular Medicine Reports, 16, 639-646. https://doi.org/10.3892/mmr.2017.6649
MLA
Wang, Y., Lai, S., Tang, J., Feng, C., Liu, F., Su, C., Zou, W., Chen, H., Xu, D."Prostaglandin E2 promotes human CD34+ cells homing through EP2 and EP4 in vitro". Molecular Medicine Reports 16.1 (2017): 639-646.
Chicago
Wang, Y., Lai, S., Tang, J., Feng, C., Liu, F., Su, C., Zou, W., Chen, H., Xu, D."Prostaglandin E2 promotes human CD34+ cells homing through EP2 and EP4 in vitro". Molecular Medicine Reports 16, no. 1 (2017): 639-646. https://doi.org/10.3892/mmr.2017.6649