Open Access

Differential expression of miRNAs in Osborne's ligament of cubital tunnel syndrome

  • Authors:
    • Xian‑Hu Zhou
    • Yi‑Ming Ren
    • Zhi‑Jian Wei
    • Wei Lin
    • Bao‑You Fan
    • Shen Liu
    • Yan Hao
    • Gui‑Dong Shi
    • Shi‑Qing Feng
  • View Affiliations

  • Published online on: May 31, 2017     https://doi.org/10.3892/mmr.2017.6663
  • Pages: 687-695
  • Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cubital tunnel syndrome (CuTS) is the second most common peripheral nerve compression disease, however, the pathogenesis and pathology of CuTS remain to be fully elucidated. The aim of the present study was to compare the expression pattern of microRNAs (miRNAs) in pachyntic Osborne's ligament with that in control tendinous tissue, and select meaningful miRNAs for further investigation of the clinical pathological mechanism underlying CuTS. A microarray assay was performed to examine the expression profiles of miRNAs in the Osborne's ligament and control tendinous tissues. An online bioinformatics algorithms tool (miRWalk) was used to predict putative target genes for the deregulated miRNAs, and functional annotation was performed by Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Finally, the results of microarray were partially validated using reverse transcription‑quantitative polymerase chain reaction analysis. The expression of total of 60 miRNAs were found to be significantly different between the pachyntic Osborne's ligament and control tendinous tissues. MiRWalk2.0 predicted 1,804 target genes for these miRNAs, and the GO functional analysis of the predicted genes suggested cellular mechanisms, including metabolic process, regulation of cell growth, cell cycle processes, cell division regulation, cellular metabolic process and signal transmission, were involved. Furthermore, KEGG pathway analysis revealed important pathways, including adherent junction, focal adhesion, lysine degradation, cell adhesion molecules and mitogen‑activated protein kinase. Compared with the heathy tissue, Osborne's ligament tissue from patients with CuTS showed a markedly different miRNA expression profile, which suggested that miRNAs may be involved in the pathogenesis of CuTS.
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July-2017
Volume 16 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhou XH, Ren YM, Wei ZJ, Lin W, Fan BY, Liu S, Hao Y, Shi GD and Feng SQ: Differential expression of miRNAs in Osborne's ligament of cubital tunnel syndrome. Mol Med Rep 16: 687-695, 2017
APA
Zhou, X., Ren, Y., Wei, Z., Lin, W., Fan, B., Liu, S. ... Feng, S. (2017). Differential expression of miRNAs in Osborne's ligament of cubital tunnel syndrome. Molecular Medicine Reports, 16, 687-695. https://doi.org/10.3892/mmr.2017.6663
MLA
Zhou, X., Ren, Y., Wei, Z., Lin, W., Fan, B., Liu, S., Hao, Y., Shi, G., Feng, S."Differential expression of miRNAs in Osborne's ligament of cubital tunnel syndrome". Molecular Medicine Reports 16.1 (2017): 687-695.
Chicago
Zhou, X., Ren, Y., Wei, Z., Lin, W., Fan, B., Liu, S., Hao, Y., Shi, G., Feng, S."Differential expression of miRNAs in Osborne's ligament of cubital tunnel syndrome". Molecular Medicine Reports 16, no. 1 (2017): 687-695. https://doi.org/10.3892/mmr.2017.6663