Overexpression of GLP-1 receptors suppresses proliferation and cytokine release by airway smooth muscle cells of patients with chronic obstructive pulmonary disease via activation of ABCA1

  • Authors:
    • Yan‑Hong Sun
    • Lan He
    • Ming‑Yu Yan
    • Rui‑Qian Zhao
    • Bin Li
    • Feng Wang
    • Ying Yang
    • Hua‑Peng Yu
  • View Affiliations

  • Published online on: May 25, 2017     https://doi.org/10.3892/mmr.2017.6618
  • Pages: 929-936
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Abstract

Glucagon-like peptide-1 (GLP‑1) is an important insulin secretagogue that possesses anti‑inflammatory effects. GLP‑1 receptor (GLP‑1R) agonists have been demonstrated to serve a pivotal role in the treatment of obstructive lung diseases, including chronic obstructive pulmonary disease (COPD). However, the specific function and underlying mechanisms of GLP‑1R in COPD remain uncertain. The aim of the present study was to investigate the action and underlying mechanisms of GLP‑1R in airway smooth muscle (ASM) cells from COPD patients. GLP‑1R expression levels were markedly decreased in ASM cells from COPD patients compared with those from healthy controls. ASM cell proliferation and migration, and the levels of the inflammatory cytokines interleukin (IL)‑1β, IL‑4, tumor necrosis factor (TNF)‑α, and granulocyte‑macrophage colony‑stimulating factor (GM‑CSF) were measured. Transfection of pcDNA3.1‑GLP‑1R had inhibitory effects on ASM cell proliferation and migration, whereas GLP‑1R small interfering (si)RNA reversed these effects. Furthermore, the present study demonstrated that GLP‑1R overexpression markedly suppressed IL‑1β, IL‑4, TNF‑α and GM‑CSF levels. GLP‑1R overexpression upregulated the expression levels of adenosine triphosphate‑binding cassette, subfamily A, member 1 (ABCA1) in ASM cells, and the effects of GLP‑1R on cell proliferation and migration, and inflammatory cytokine expression in ASM cells was abolished by siRNA‑mediated silencing of ABCA1. The results of the present study suggested that GLP‑1R contributes to COPD pathology, potentially via an ABCA1‑mediated pathway.
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July-2017
Volume 16 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Sun YH, He L, Yan MY, Zhao RQ, Li B, Wang F, Yang Y and Yu HP: Overexpression of GLP-1 receptors suppresses proliferation and cytokine release by airway smooth muscle cells of patients with chronic obstructive pulmonary disease via activation of ABCA1. Mol Med Rep 16: 929-936, 2017
APA
Sun, Y., He, L., Yan, M., Zhao, R., Li, B., Wang, F. ... Yu, H. (2017). Overexpression of GLP-1 receptors suppresses proliferation and cytokine release by airway smooth muscle cells of patients with chronic obstructive pulmonary disease via activation of ABCA1. Molecular Medicine Reports, 16, 929-936. https://doi.org/10.3892/mmr.2017.6618
MLA
Sun, Y., He, L., Yan, M., Zhao, R., Li, B., Wang, F., Yang, Y., Yu, H."Overexpression of GLP-1 receptors suppresses proliferation and cytokine release by airway smooth muscle cells of patients with chronic obstructive pulmonary disease via activation of ABCA1". Molecular Medicine Reports 16.1 (2017): 929-936.
Chicago
Sun, Y., He, L., Yan, M., Zhao, R., Li, B., Wang, F., Yang, Y., Yu, H."Overexpression of GLP-1 receptors suppresses proliferation and cytokine release by airway smooth muscle cells of patients with chronic obstructive pulmonary disease via activation of ABCA1". Molecular Medicine Reports 16, no. 1 (2017): 929-936. https://doi.org/10.3892/mmr.2017.6618