Open Access

Microarray analysis reveals key genes and pathways in Tetralogy of Fallot

  • Authors:
    • Yue‑E He
    • Hui‑Xian Qiu
    • Jian‑Bing Jiang
    • Rong‑Zhou Wu
    • Ru‑Lian Xiang
    • Yuan‑Hai Zhang
  • View Affiliations

  • Published online on: July 6, 2017     https://doi.org/10.3892/mmr.2017.6933
  • Pages: 2707-2713
  • Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to identify key genes that may be involved in the pathogenesis of Tetralogy of Fallot (TOF) using bioinformatics methods. The GSE26125 microarray dataset, which includes cardiovascular tissue samples derived from 16 children with TOF and five healthy age‑matched control infants, was downloaded from the Gene Expression Omnibus database. Differential expression analysis was performed between TOF and control samples to identify differentially expressed genes (DEGs) using Student's t‑test, and the R/limma package, with a log2 fold‑change of >2 and a false discovery rate of <0.01 set as thresholds. The biological functions of DEGs were analyzed using the ToppGene database. The ReactomeFIViz application was used to construct functional interaction (FI) networks, and the genes in each module were subjected to pathway enrichment analysis. The iRegulon plugin was used to identify transcription factors predicted to regulate the DEGs in the FI network, and the gene‑transcription factor pairs were then visualized using Cytoscape software. A total of 878 DEGs were identified, including 848 upregulated genes and 30 downregulated genes. The gene FI network contained seven function modules, which were all comprised of upregulated genes. Genes enriched in Module 1 were enriched in the following three neurological disorder‑associated signaling pathways: Parkinson's disease, Alzheimer's disease and Huntington's disease. Genes in Modules 0, 3 and 5 were dominantly enriched in pathways associated with ribosomes and protein translation. The Xbox binding protein 1 transcription factor was demonstrated to be involved in the regulation of genes encoding the subunits of cytoplasmic and mitochondrial ribosomes, as well as genes involved in neurodegenerative disorders. Therefore, dysfunction of genes involved in signaling pathways associated with neurodegenerative disorders, ribosome function and protein translation may contribute to the pathogenesis of TOF.
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September-2017
Volume 16 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
He YE, Qiu HX, Jiang JB, Wu RZ, Xiang RL and Zhang YH: Microarray analysis reveals key genes and pathways in Tetralogy of Fallot. Mol Med Rep 16: 2707-2713, 2017
APA
He, Y., Qiu, H., Jiang, J., Wu, R., Xiang, R., & Zhang, Y. (2017). Microarray analysis reveals key genes and pathways in Tetralogy of Fallot. Molecular Medicine Reports, 16, 2707-2713. https://doi.org/10.3892/mmr.2017.6933
MLA
He, Y., Qiu, H., Jiang, J., Wu, R., Xiang, R., Zhang, Y."Microarray analysis reveals key genes and pathways in Tetralogy of Fallot". Molecular Medicine Reports 16.3 (2017): 2707-2713.
Chicago
He, Y., Qiu, H., Jiang, J., Wu, R., Xiang, R., Zhang, Y."Microarray analysis reveals key genes and pathways in Tetralogy of Fallot". Molecular Medicine Reports 16, no. 3 (2017): 2707-2713. https://doi.org/10.3892/mmr.2017.6933