Open Access

MRI reveals slow clearance of dead cell transplants in mouse forelimb muscles

  • Authors:
    • Yanhui Zhang
    • Hongyan Zhang
    • Lijun Ding
    • Hailu Zhang
    • Pengli Zhang
    • Haizhen Jiang
    • Bo Tan
    • Zongwu Deng
  • View Affiliations

  • Published online on: July 27, 2017     https://doi.org/10.3892/mmr.2017.7100
  • Pages: 4068-4074
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

A small molecule tetraazacyclododecane-1,4,7,10-tetraacetic acid (Gd‑DOTA)4‑TPP agent is used to label human mesenchymal stem cells (hMSCs) via electroporation (EP). The present study assessed the cytotoxicity of cell labeling, in addition to its effect on cell differentiation potential. There were no significant adverse effects on cell viability or differentiation induced by either EP or cellular uptake of (Gd‑DOTA)4‑TPP. Labeled live and dead hMSCs were transplanted into mouse forelimb muscles. T2‑weighted magnetic resonance imaging (MRI) was used to track the in vivo fate of the cell transplants. The labeling and imaging strategy allowed long term tracking of the cell transplants and unambiguous distinguishing of the cell transplants from their surrounding tissues. Cell migration was observed for live hMSCs injected into subcutaneous tissues, however not for either live or dead hMSCS injected into limb muscles. A slow clearance process occurred of the dead cell transplants in the limb muscular tissue. The MRI results therefore reveal that the fate and physiological activities of cell transplants depend on the nature of their host tissue.

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October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Zhang Y, Zhang H, Ding L, Zhang H, Zhang P, Jiang H, Tan B and Deng Z: MRI reveals slow clearance of dead cell transplants in mouse forelimb muscles. Mol Med Rep 16: 4068-4074, 2017
APA
Zhang, Y., Zhang, H., Ding, L., Zhang, H., Zhang, P., Jiang, H. ... Deng, Z. (2017). MRI reveals slow clearance of dead cell transplants in mouse forelimb muscles. Molecular Medicine Reports, 16, 4068-4074. https://doi.org/10.3892/mmr.2017.7100
MLA
Zhang, Y., Zhang, H., Ding, L., Zhang, H., Zhang, P., Jiang, H., Tan, B., Deng, Z."MRI reveals slow clearance of dead cell transplants in mouse forelimb muscles". Molecular Medicine Reports 16.4 (2017): 4068-4074.
Chicago
Zhang, Y., Zhang, H., Ding, L., Zhang, H., Zhang, P., Jiang, H., Tan, B., Deng, Z."MRI reveals slow clearance of dead cell transplants in mouse forelimb muscles". Molecular Medicine Reports 16, no. 4 (2017): 4068-4074. https://doi.org/10.3892/mmr.2017.7100