Protein interacting with C‑kinase 1 modulates exocytosis and KATP conductance in pancreatic β cells

  • Authors:
    • Yunhong Li
    • Fan Li
    • Bin Bai
    • Zhenyong Wu
    • Xiaolin Hou
    • Ying Shen
    • Yin Wang
  • View Affiliations

  • Published online on: July 21, 2017     https://doi.org/10.3892/mmr.2017.7056
  • Pages: 4247-4252
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Abstract

It has been previously identified that α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptors (AMPARs) are expressed in pancreatic β cells and regulate exocytosis and insulin release. It is known that protein interacting with C‑kinase 1 (PICK1) regulates trafficking and synaptic targeting of AMPARs in the central nervous system. However, it is unknown whether PICK1 regulates glutamate‑induced insulin release in β cells. The present study demonstrated that glutamate‑induced exocytosis was increased in β cells derived from PICK1‑knockout mice. In agreement with this result, adding PICK1 in β cells reduced glutamate‑induced exocytosis, whereas adding EVKI, a peptide that interrupts the interaction between AMPARs and PICK1, increased the exocytosis of β cells with the application of glutamate. Furthermore, the conductance of ATP‑sensitive potassium (KATP) channels was reduced in PICK1‑knockout mice, which was reversed by the overexpression of PICK1. In addition, PICK1 application reduced voltage oscillation induced by the closure of KATP. Taken together, the results indicate that PICK1 regulates glutamate‑induced exocytosis in β cells.

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October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Li Y, Li F, Bai B, Wu Z, Hou X, Shen Y and Wang Y: Protein interacting with C‑kinase 1 modulates exocytosis and KATP conductance in pancreatic β cells. Mol Med Rep 16: 4247-4252, 2017
APA
Li, Y., Li, F., Bai, B., Wu, Z., Hou, X., Shen, Y., & Wang, Y. (2017). Protein interacting with C‑kinase 1 modulates exocytosis and KATP conductance in pancreatic β cells. Molecular Medicine Reports, 16, 4247-4252. https://doi.org/10.3892/mmr.2017.7056
MLA
Li, Y., Li, F., Bai, B., Wu, Z., Hou, X., Shen, Y., Wang, Y."Protein interacting with C‑kinase 1 modulates exocytosis and KATP conductance in pancreatic β cells". Molecular Medicine Reports 16.4 (2017): 4247-4252.
Chicago
Li, Y., Li, F., Bai, B., Wu, Z., Hou, X., Shen, Y., Wang, Y."Protein interacting with C‑kinase 1 modulates exocytosis and KATP conductance in pancreatic β cells". Molecular Medicine Reports 16, no. 4 (2017): 4247-4252. https://doi.org/10.3892/mmr.2017.7056