Inhibitory effects of FKBP14 on human cervical cancer cells

  • Authors:
    • Lian‑Yi Sun
    • Jiu‑Zhi Tao
    • Bing Yan
    • Jian‑Shu Lin
  • View Affiliations

  • Published online on: July 21, 2017     https://doi.org/10.3892/mmr.2017.7043
  • Pages: 4265-4272
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Abstract

The FK506-binding protein 14 (FKBP14), which belongs to a subfamily of immunophilins, has been implicated in various biochemical processes. However, its effects on human cervical cancer remain to be elucidated. The present study aimed to determine the exact role of FKBP14 in human cervical cancer cell proliferation, cell cycle progression, apoptosis, invasion and migration. Cell proliferation was measured by Cell Counting Kit‑8 assay. Flow cytometry was conducted to determine the effects of FKBP14 on cell cycle progression and apoptosis. Cell invasion and migration were determined by Transwell assay. The results of the present study demonstrated that silencing FKBP14 expression using short hairpin (sh)RNA suppressed proliferation, invasion and migration of HeLa and C‑33A cells, and also induced apoptosis and cell cycle arrest. Furthermore, silencing FKBP14 expression decreased the protein expression levels of B‑cell lymphoma 2 (Bcl‑2), matrix metalloproteinase (MMP)2 and MMP9, and increased the levels of caspase‑3 and Bcl‑2‑associated X protein in FKBP14 shRNA‑infected HeLa and C‑33A cells. In conclusion, FKBP14 may act as an oncogene through suppressing apoptosis and promoting motility in human cervical carcinogenesis; therefore, it may be considered a potential therapeutic target for the treatment of cervical cancer.

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October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Sun LY, Tao JZ, Yan B and Lin JS: Inhibitory effects of FKBP14 on human cervical cancer cells. Mol Med Rep 16: 4265-4272, 2017
APA
Sun, L., Tao, J., Yan, B., & Lin, J. (2017). Inhibitory effects of FKBP14 on human cervical cancer cells. Molecular Medicine Reports, 16, 4265-4272. https://doi.org/10.3892/mmr.2017.7043
MLA
Sun, L., Tao, J., Yan, B., Lin, J."Inhibitory effects of FKBP14 on human cervical cancer cells". Molecular Medicine Reports 16.4 (2017): 4265-4272.
Chicago
Sun, L., Tao, J., Yan, B., Lin, J."Inhibitory effects of FKBP14 on human cervical cancer cells". Molecular Medicine Reports 16, no. 4 (2017): 4265-4272. https://doi.org/10.3892/mmr.2017.7043